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Eurotium cristatum strain and domestication method and fermentation method thereof

A technology of R. coronoides and strains, which is applied in the fields of R. coronoidans strains and domestication and fermentation, and can solve the problems of low absorption rate, irritation, and large side effects in the gastrointestinal tract.

Pending Publication Date: 2021-03-16
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In daily life, people mainly take in iron in the form of inorganic state, but its use is limited due to the stimulation of iron ions on the mucous membrane, the side effects of the gastrointestinal tract, and the low absorption rate.

Method used

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  • Eurotium cristatum strain and domestication method and fermentation method thereof
  • Eurotium cristatum strain and domestication method and fermentation method thereof
  • Eurotium cristatum strain and domestication method and fermentation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 Screening of the Selenium-enriched wild bacterial strain of Saccharomyces coronoides

[0066] Weigh 10g of Fuzhuan tea with bright yellow closed cystic shell from the commercially available "Jingwei Fucha" in Xi'an, put it into a mortar and roughly crush it, then add 90mL of sterile water with 3-5 glass beads in the Erlenmeyer flask. Shake and mix at 150rpm for about 30min. Take 1mL of the spore mixture and make a 10-fold gradient dilution with sterile water, and select 10 -3 and 10 -4 Draw 0.1 mL of the two gradient spore suspensions and spread it on the PDA solid medium containing 10 mg / L selenium element, and culture it statically at 30°C for 60 hours. Pick larger colonies and bright yellow colonies and streak them on a PDA plate containing 10mg / L selenium to continue screening for 8-10 generations, culture on a PDA slant, and store at 4°C.

Embodiment 2

[0067] Mutagenesis, domestication and isolation and purification of the wild bacterial strain of embodiment 2

[0068] The bacterial lawn screened in Example 1 was eluted with sterile physiological saline, dispersed with glass beads, and the concentration was adjusted to 2×10 6 -2×10 8 spores / mL of spore suspension, draw a certain amount of spore suspension and place it in a petri dish (so that the liquid level does not exceed 3mm), irradiate with a 20W ultraviolet lamp tube for 25min at a distance of 30cm, and draw 0.1mL of bacterial suspension into the culture dish. Containing different concentrations (0; 5; 10; 15; 20; 25mg / L) of inorganic selenium in the PDA solid plate and coating. Incubate at 30°C for 60h. Select a few colonies with large colonies and bright colors on different concentrations of selenium to streak on the PDA solid plate containing 10mg / L inorganic selenium, and compare the colony size and colony appearance again. Select the largest colony and the brigh...

Embodiment 3

[0070] Example 3 Preservation of the Selenium-enriched strain of Saccharomyces coronoides

[0071] Pick the selenium-enriched Saccharomyces coronaris bacterial lawn screened in Example 2 on a solid PDA slope, cultivate it at 30°C for about 60 hours, add 20mL of sterile water and scrape the bacterial lawn with a bamboo stick, and the spore concentration after mixing is about 2×10 6 --2×10 8 individual / mL. Add PDA liquid medium containing 50% glycerol at a volume ratio of 1:1, and mix well. Pre-freeze at -20°C first, and then transfer to -80°C for medium and long-term storage. The spore germination rate is above 90%.

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Abstract

The invention discloses an eurotium cristatum strain preserved in China General Microbiological Culture Collection Center on April 2, 2018, and the eurotium cristatum strain is Eurotium Cristatum XD-01 with the preservation number of CGMCC No.15395, Eurotium Cristatum XD-02 with the preservation number of CGMCC No.15396 or Eurotium Cristatum XD-03 with the preservation number of CGMCC No.15397. Inaddition, the invention also discloses a domestication method and a fermentation method of the eurotium cristatum strain. The eurotium cristatum strain disclosed by the invention is derived from Fuzhuan tea, and is ecologically safe and harmless.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a strain of Saccharomyces coronoides and a domestication and fermentation method. Background technique [0002] Selenium is an essential trace element for the human body. Selenium has the functions of anti-oxidation, scavenging free radicals, protecting the heart, preventing myopia and cataracts, AIDS, diabetes, and cancer. The World Health Organization has clear regulations on the daily selenium intake of the human body. The Chinese Nutrition Society stipulates that the daily intake of selenium for adults is 50-250 micrograms. In my country, 70% of the land is deficient in selenium, and the daily intake of selenium for adults is about 20-30 micrograms. Therefore, the safe supplementation of selenium has been widely studied, especially the conversion of inorganic selenium into organic selenium by probiotics. The reported probiotics that can enrich seleniu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/36C12N15/01C12P9/00C12R1/645
CPCC12N1/14C12N1/36C12N15/01C12P9/00
Inventor 范代娣惠俊峰段志广徐茹唐育岐宋养斌
Owner NORTHWEST UNIV(CN)
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