Cosmetic composition comprising rose stem cell-derived exosome as effective ingredient

A cosmetic composition and stem cell technology, applied in the direction of cosmetics, cosmetic preparations, dressing preparations, etc., can solve problems such as foreign body sensation, side effects, and short duration of action

Active Publication Date: 2021-03-16
韩国外泌体生技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, functional cosmetics containing plant extracts as active ingredients have problems that they may cause foreign body sensation during their evaporation after application to the skin, and their action duration is short
In addition, the conditioned medium of rose callus contains a growth regulator or a callus-inducing substance, and thus is hardly considered a natural cosmetic ingredient, and the growth regulator contained in the culture may cause side effects such as skin problems

Method used

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  • Cosmetic composition comprising rose stem cell-derived exosome as effective ingredient
  • Cosmetic composition comprising rose stem cell-derived exosome as effective ingredient
  • Cosmetic composition comprising rose stem cell-derived exosome as effective ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Preparation of rose stem cells

[0045] According to the preparation and culture methods of plant stem cells known in the art, callus tissue is induced from rose embryos and / or leaves, and cells of the induced callus tissue are cultured. In addition, the callus with good growth state was selected and cultured in large quantities, so as to prepare the conditioned medium of rose stem cells.

Embodiment 2

[0046] Example 2: Preparation of exosomes derived from rose stem cells

[0047] The conditioned medium of rose stem cells prepared as described in Example 1 was purchased from BIO-FD&C Co., Ltd. (located in Incheon, Korea, and supplying conditioned medium of rose Damascena stem cells). Filter the conditioned medium of rose stem cells through a 0.22 µm filter to remove impurities such as cell debris, waste, and large particles. Rose stem cell-derived exosomes were isolated from filtered conditioned medium by tangential flow filtration (TFF).

[0048] The size of isolated rose stem cell-derived exosomes was analyzed by transmission electron microscopy (TEM). As shown in FIGS. 1A and 1B , isolated rose stem cell-derived exosomes were determined to be nano-sized vesicles. The size and concentration of rose stem cell-derived exosomes were analyzed by nanoparticle tracking analysis (NTA) using NS300 (purchased from Malvern Panalytical) ( figure 2 ).

Embodiment 3

[0049] Example 3: Evaluation of the delivery ability of rose stem cell-derived exosomes into dermal fibroblasts

[0050] In order to examine whether rose stem cell-derived exosomes would be delivered to human dermal fibroblasts (purchased from CEFO Co., Ltd.), the following analysis was performed. In order to fluorescently stain the membrane of rose stem cell-derived exosomes prepared in Example 2, the exosomes were reacted with PKH67 fluorescent dye (purchased from Sigma-Aldrich). After the reaction, the reaction solution was fractionated with a MW3000 column (purchased from ThermoFisher Scientific) to remove unstained free PHK67 in the exosome membrane. A negative control was prepared by reacting PKH67 fluorescent dye with buffer solution and fractionating the reaction product with a MW3000 column. Exosomes stained with PKH67 were incubated with pre-cultured human dermal fibroblasts, and fluorescence microscopy was then used to observe whether exosomes would be delivered in...

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PUM

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Abstract

The present invention provides a cosmetic composition comprising rose stem cell-derived exosomes as an effective ingredient for skin regeneration, skin elasticity improvement or skin wrinkle reduction. The cosmetic composition of the present invention has excellent effects on skin regeneration, skin elasticity improvement and / or skin wrinkle reduction.

Description

technical field [0001] The present invention relates to a cosmetic composition for skin regeneration, improvement of skin elasticity and reduction of skin wrinkles, comprising rose stem cell-derived exosomes as an active ingredient. Background technique [0002] Skin aging is known to result in decreased skin elasticity and increased skin wrinkles, and decreased skin elasticity and skin wrinkle formation are known to occur due to decreased collagen synthesis and stimulated expression of collagenase matrix metalloproteinase (MMP). [0003] In addition, it is known that in skin cells, the enzyme COX-2, which produces inflammatory cytokines, increases due to aging or ultraviolet (UV) rays, leading to increased synthesis of prostaglandin E2 and increased production of inflammation inducers. Due to the inflammatory response, the biosynthesis of MMP is increased, leading to the degradation of collagen and leading to the decrease of skin elasticity and the formation of skin wrinkle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/02A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/0208A61K8/0212A61K8/9789A61Q19/08A61K8/14A61Q19/00A61Q19/02A61K8/0241A61K8/042A61K8/044A61K8/046A61K8/06
Inventor 李龙源赵柄成
Owner 韩国外泌体生技有限公司
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