Amino acid saturation type amnion moisturizing mask and preparation method thereof
A technology of amino acid and amniotic membrane, which is applied in the fields of pharmaceutical formulations, cosmetic preparations, dressing preparations, etc., can solve the problems of safety and effectiveness beyond doubt, and achieve the effects of improving skin metabolism, enhancing hydrating effect, and having a wide range of sources
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[0052] Take goat placenta as an example below.
[0053] (1) Separation of amnion and chorion:
[0054] The healthy goat placenta was washed 3 times with water first, and then washed 2 times with phosphate buffered saline pre-cooled at 4°C; the amnion and chorion were bluntly separated, and the separated amnion and chorion were washed with distilled water 3 times again.
[0055] (2) Amniotic membrane pretreatment:
[0056] ①Preparation of 1% TritonX-100: Mix TritonX-100 stock solution and PBS at a volume ratio of 3:7, place in a 37°C water bath for 3 hours to fully dissolve and mix, and prepare a 30% TritonX-100 stock solution; This stock solution was diluted 30 times to make 1% TritonX-100 solution;
[0057] ② Amniotic membrane pretreatment: soak the isolated amniotic membrane in 1% TritonX-100PBS solution, shake at 4°C for 24 hours, take it out and wash it.
[0058] Treating the amniotic membrane with TritonX-100 has the effect of dissolving lipids and increasing the perme...
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