The invention relates to the technical field of
probiotic preparations, in particular to a
probiotic preparation with a
helicobacter pylori
antagonism function and application of the
probiotic preparation. The preparation method comprises the following steps: S1,
drying selenium-enriched corn, and performing crushing to obtain
powder; s2, adding the
powder into an
ethanol solution, and carrying out water bath
reflux extraction to obtain an extracting solution; s3, filtering the extracting solution, and adding an
ethanol solution into the filtrate until the final concentration is 70%; s4, performing centrifuging, evaporating the supernatant to
dryness to obtain an extract, and reserving the filtrate for later use; s5, fixing the volume of the filtrate to 50ml, adding
activated carbon, performing stirring, filtering, and concentrating to obtain a free
selenium amino acid concentrated solution; s6, culturing
lactobacillus helveticus in an MRS culture medium to obtain a bacterium solution;s7, centrifuging the bacterial liquid to obtain precipitate, and centrifugally washing the precipitate twice to obtain bacterial
sludge; and S8, resuspending the prepared bacterial
sludge with normalsaline with the same volume, adding 10-15g of brown
sugar, 10-15g of
skimmed milk powder, 500-600g of free
selenium amino acid concentrated solution, 2-5g of
vitamin E and 2-5g of iron supplementingagent into every 100mL of bacterial suspension, uniformly performing mixing, and carrying out vacuum
freeze drying to obtain a finished product.