Method of preparing glutathione freeze-dried powder

A technology of glutathione and freeze-dried powder, which is applied in the field of glutathione preparation, can solve the problems of increasing waste liquid treatment process equipment and sites, reducing the utilization rate of GSH crystals, and low GSH purity, so as to save production raw materials, The effect of less equipment and convenient use

Inactive Publication Date: 2008-12-10
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: 1. produce a large amount of eluted waste liquid with strong acidity and alkalinity, which must be purified before being discharged, which not only increases the production cost, but also increases the process equipment and site for treating the waste liquid, thereby further increasing the production cost. Cost of production
②The crystallization process of this method ofte...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The specific method steps of a method for preparing glutathione freeze-dried powder are as follows:

[0031] (1) Preparation of GSH extract

[0032] Using bread dry yeast as raw material, according to the ratio of weight of bread dry yeast: volume of distilled water: volume of xylene is 1:2:0.03, first add distilled water to bread dry yeast, stir and mix well. Then add xylene, stir and mix well, place on a shaker and shake for 5 hours. Then add the same volume of distilled water as the mixed solution, and then perform suction filtration, discard the filter residue, and collect the filtrate. Adjust the pH of the filtrate to 1.0 with dilute hydrochloric acid, add solid sodium chloride, stir and mix uniformly to prepare a reduced glutathione (GSH) extract with a molar concentration of sodium chloride of 0.05 mol / L.

[0033] (2) Preparation of reverse micelle solution

[0034] Dioctyl phosphate (P 204 ) Is the extractant, with n-octanol as the diluent, the P 204 Add to n-octano...

Embodiment 2

[0046] The specific method steps of a method for preparing glutathione freeze-dried powder are as follows:

[0047] (1) Preparation of GSH extract

[0048] Same as Example 1, characterized in that the weight ratio of bread dry yeast: volume of distilled water: xylene is 1:3:0.05, shaken for 8 hours, the pH is 3.0, and the molar concentration of sodium chloride is 0.2. mol / L reduced glutathione extract.

[0049] (2) Preparation of reverse micelle solution

[0050] Same as Example 1, characterized by: formulating P 204 The molar concentration of 0.2mol / L P 204 Solution, P 204 P of solution 204 : The molar ratio of distilled water is 1:20, and the mixture is stirred for 1.5 hours.

[0051] (3) Extraction

[0052] Same as Example 1, characterized in that: the GSH extract prepared in step (1): the P prepared in step (2) 204 The volume ratio of the reverse micelle solution is 1:4, stirred and extracted for 45 minutes, centrifuged at a speed of 9000 r / min for 15 minutes, and then added 4...

Embodiment 3

[0062] The specific method steps of a method for preparing glutathione freeze-dried powder are as follows:

[0063] (1) Preparation of GSH extract

[0064] Same as Example 1, characterized in that: the weight of bread dry yeast: the volume of distilled water: the volume of xylene is 1:1.5:0.04, shake for 6 hours, adjust the pH to 2.0, and prepare the molar concentration of sodium chloride to 0.1 mol / L reduced glutathione extract.

[0065] (2) Preparation of reverse micelle solution

[0066] Same as Example 1, characterized in that: P is formulated 204 The molar concentration of P is 0.16mol / L 204 Solution, P 204 P of the solution 204 The molar ratio of :distilled water is 1:10, and the mixture is stirred for 1 hour.

[0067] (3) Extraction

[0068] Same as Example 1, characterized in that: the GSH extract prepared in step (1): the P prepared in step (2) 204The volume ratio of the reverse micelle solution is 1:3, stirred and extracted for 30 minutes, centrifuged at a speed of 8000r...

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PUM

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Abstract

The invention discloses a method for preparing glutathione freeze-dried powder, which relates to a method for preparing reduced glutathione (GSH) freeze-dried powder by a reverse micelle extraction method. The method takes bread dry yeast as a raw material to prepare extract containing GSH and a P204 reverse micelle solution first, then the GSH extract is extracted through the reverse micelle solution, then a sodium chloride solution is used to back-extract the reverse micelle solution after the extraction, and then a finished product is prepared through hyperfiltration and freeze drying. The method is simple and has mild preparation conditions, the back-extracted reverse micelle solution can be regenerated and utilized, the method has low production cost; in the prepared freeze-dried powder, the content of the GSH is high up to 95.2 percent, the recovery rate is high up to 86.3 percent, and a compound amino acid concentrated solution byproduct can be prepared. The GSH freeze-dried powder prepared by the method can be widely used as antioxidant preservatives and flavor additives in pharmaceutical raw materials and food industry, and can also be used as feed additives and bread yeast culture media and so on.

Description

Technical field [0001] The invention belongs to the technical field of glutathione preparation, and particularly relates to a method for preparing reduced glutathione freeze-dried powder by a reverse micelle extraction method. technical background [0002] Glutathione is a very important active tripeptide. There are two forms of reduced form and oxidized form. It is widely present in animals, plants and microorganisms. The main physiological functions are to resist free radical damage, detoxify, promote cell protein synthesis, participate in the transmethylation or propylamino reaction, and maintain the normal function of liver cells. Therefore, glutathione is widely used in the medical field, such as the treatment of liver disease, acute and chronic renal failure, cardiovascular disease, senile eye disease, diabetic nerve damage, liver protection and intestinal protection after tumor radiotherapy, etc.; glutathione Peptides are also widely used in the food industry, such as nood...

Claims

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Application Information

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IPC IPC(8): C12P21/02C07K1/14A61K38/06A23L3/3535A23L1/226A23K1/16C12R1/85A23K20/147A23L27/20
Inventor 周小华王东郑申声
Owner CHONGQING UNIV
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