Probiotic preparation with helicobacter pylori antagonism function and application thereof

Abstract

The invention relates to the technical field of probiotic preparations, in particular to a probiotic preparation with a helicobacter pylori antagonism function and application of the probiotic preparation. The preparation method comprises the following steps: S1, drying selenium-enriched corn, and performing crushing to obtain powder; s2, adding the powder into an ethanol solution, and carrying out water bath reflux extraction to obtain an extracting solution; s3, filtering the extracting solution, and adding an ethanol solution into the filtrate until the final concentration is 70%; s4, performing centrifuging, evaporating the supernatant to dryness to obtain an extract, and reserving the filtrate for later use; s5, fixing the volume of the filtrate to 50ml, adding activated carbon, performing stirring, filtering, and concentrating to obtain a free selenium amino acid concentrated solution; s6, culturing lactobacillus helveticus in an MRS culture medium to obtain a bacterium solution;s7, centrifuging the bacterial liquid to obtain precipitate, and centrifugally washing the precipitate twice to obtain bacterial sludge; and S8, resuspending the prepared bacterial sludge with normalsaline with the same volume, adding 10-15g of brown sugar, 10-15g of skimmed milk powder, 500-600g of free selenium amino acid concentrated solution, 2-5g of vitamin E and 2-5g of iron supplementingagent into every 100mL of bacterial suspension, uniformly performing mixing, and carrying out vacuum freeze drying to obtain a finished product.

Description

technical field [0001] The invention relates to the technical field of probiotic preparations, in particular to a probiotic preparation with the function of antagonizing Helicobacter pylori and its application. Background technique [0002] Helicobacter pylori is a pathogenic bacterium of chronic gastritis and peptic ulcer, which is closely related to gastric cancer. WHO has listed it as a class I carcinogen. The infection rate of HP in the population is as high as 50%. At present, triple drugs containing antibiotics (bismuth or proton pump inhibitors + metronidazole + antibiotics) are mainly used clinically to treat Helicobacter pylori infection, but many patients have serious side effects. , Poor compliance; multiple antibacterial drugs will disturb the microecological balance in the gastrointestinal tract; especially antibiotic therapy is prone to drug resistance. [0003] Compared with antibiotics, probiotics have obvious advantages in the treatment of Hp. Existing stu...

AI Technical Summary

Problems solved by technology

[0007] However, how to apply selenium and probiotics to inhibit Helicobacter pylori at the same time has not been reported yet.

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