Amino acid concentrate preparation method and amino acid-containing feed
A technology of amino acid and concentrated solution, which is applied in animal feed, additional food elements, animal husbandry, etc., can solve the problems of no win-win situation, limited animal absorption, protein consumption, etc., achieves huge economic value and environmental protection value, and is conducive to transportation and The effect of simple storage and operation steps
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Embodiment 1
[0064] Embodiment 1: take by weighing 500g of glutamic acid bacterium slag, be dissolved in 1500 milliliters of water, become the concentration suspension of 25%, add the sodium hydroxide solution of 80mL 20% concentration to destroy cell wall, remove heating device cooling after solution is heated to boiling Let it stand at room temperature for 24 hours, take a sample to develop color with the ninhydrin method, and amino acid spots appear.
Embodiment 2
[0065] Embodiment 2: Weigh 500 g of glutamic acid bacteria residue, dissolve it in 1500 milliliters of water, and become a suspension with a concentration of 25%, use NaOH aqueous solution to adjust the pH between 9-10, add alkaline protease to hydrolyze at 50 ° C 2 hours. Treat 200 ml of the solution with 2000 ml of alkali, add 2 g of alkaline protease and hydrolyze it at 50°C for 2 hours, filter the amino acid aqueous solution, adjust the pH of the amino acid aqueous solution to 0-1, pass through a cation resin exchange column, and remove impurities with water Wash it off, then elute the cation resin exchange column with 0.5-1.5mol / L ammonia water, then concentrate the eluate in vacuum to obtain a product containing 50% amino acid.
Embodiment 3
[0066] Example 3: 1000g of pig blood meal was dissolved in 2000mL of 6 mol / L hydrochloric acid, and hydrolyzed in a boiling state for 24 hours. The total amount of amino acids reached 820g after analysis. Moreover, industrial feeding production of tons of batches has been realized in industrial production, and the effect is satisfactory.
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