Embryo culture and monitoring system

Abstract

The invention discloses an embryo culture and monitoring system. The embryo culture and monitoring system comprises a gas supply module, an embryo culture module and an optical monitoring module; theembryo culture module comprises at least one embryo culture chamber, a culture solution balancing chamber and a liquid path assembly; the embryo culture chamber is capable of independently carrying out embryo culture; the culture solution balancing chamber is used for providing a culture solution for the embryo culture chamber; the liquid path assembly is used for conveying the culture solution; the gas supply module comprises a multi-path gas source supply unit and a gas mixing device; the multi-path gas source supply unit is used for providing various gases required by embryo culture; the gas mixing device is used for mixing the various gases provided by the multi-path gas source supply unit according to a specific proportion and then conveying the mixed gases to the embryo culture chamber; and the optical monitoring module is used for monitoring an embryo in the embryo culture chamber. The embryo culture and monitoring system disclosed by the invention can realize online adjustmentof concentrations of various gases required by embryo culture and online automatic replacement of a culture solution, and can realize long-time continuous embryo culture; and the whole development cycle of embryos in multiple groups of embryo culture chambers can be monitored through the single optical monitoring module.

Description

technical field [0001] The invention relates to the field of embryo culture in vitro, in particular to an embryo culture and monitoring system. Background technique [0002] Birth defects have always been an important problem in the field of reproductive health. According to reports, the total incidence of birth defects in my country is about 5.6%. The diseases mainly include structural deformities such as congenital heart disease and cleft lip and palate, as well as genetic diseases such as phenylketonuria and severe spinal muscular atrophy. Birth defects can occur at any stage of pregnancy, especially early. With the full opening of my country's second-child policy in 2015, the prevention and treatment of birth defects has become an important research direction in the field of reproductive biology. But at present, there are still many unsolved mysteries about the physiological process of the normal development of human embryos. For example, the dynamic changes of X chro...

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Problems solved by technology

[0004] In the field of assisted reproduction, the traditional in vitro development of embryos adopts a static culture system. Generally, the embryos are cultured in a conventional incubator, and the embryos are taken out at a specific time point to observe the embryos under a microscope or perform liquid replacement operations. Interference with growth, and higher requirements for operator skills and work intensity

The development of the blastocyst in the later stage increases with the growth of the embryo size, and the O 2 The demand will gradually increase, and the components of the culture medium must be replaced in time. At the same time, a large enough optical observation field is required to image the embryo, but there is currently a lack of reliable solutions to support the above functions.

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