Unlock instant, AI-driven research and patent intelligence for your innovation.

Porcine epidemic diarrhea virus strain and inactivated vaccine and interferon combined immunization method thereof

A technology of porcine epidemic diarrhea and interferon, which is applied in the field of viruses and immunity, can solve problems such as incompatibility, and achieve the effects of good immunogenicity, good immunogenicity and stability, and wide application value

Active Publication Date: 2021-03-19
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]However, the existing vaccine strains such as CV777 and DR13 are all GI subtype strains, which shows that the current vaccine strains are no longer the same as the prevailing strains in my country. too match

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Porcine epidemic diarrhea virus strain and inactivated vaccine and interferon combined immunization method thereof
  • Porcine epidemic diarrhea virus strain and inactivated vaccine and interferon combined immunization method thereof
  • Porcine epidemic diarrhea virus strain and inactivated vaccine and interferon combined immunization method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Isolation, Identification and Sequencing Analysis of Porcine Epidemic Diarrhea Virus PEDV-CH-HB-1801 Strain

[0023] 1. Virus isolation and identification

[0024] Take the small intestine and its contents of piglets with suspected porcine epidemic diarrhea cases collected clinically, after aseptic treatment, grinding and crushing, centrifuge at 12000rpm at 4°C for 5 minutes, collect the upper layer suspension, filter through a 0.22um sterile filter, and collect filtrate. Extract RNA, use the cDNA obtained by reverse transcription as a template, and use the primers shown in SEQ ID NO.1 to amplify the target fragment of the virus. Agarose gel electrophoresis was used to identify the size of the target fragment and determine whether it was a popular strain of PEDV.

[0025] The filtrate was taken and inoculated into a monolayer of Vero cells at a ratio of 500ul per well of a 6-well plate. At the same time, an appropriate amount of trypsin was added to the cel...

Embodiment 2

[0046] Example 2 Establishment of the joint immunization method of porcine epidemic diarrhea virus PEDV-CH-HB-1801 strain and porcine genetically engineered interferon

[0047] According to the method described above, the inactivated vaccine of PEDV-CH-HB-1801 strain was prepared. At the same time, the porcine genetically engineered interferon diluted in physiological saline or astragalus polysaccharide injection was prepared respectively. The concentration of astragalus polysaccharide in the astragalus polysaccharide injection is 10 mg / mL. The porcine genetically engineered interferon is alpha interferon, and the final dilution concentration is 500,000 IU / mL.

[0048] Fifteen PEDV-negative pregnant sows were selected and divided into 5 groups with 3 pigs in each group. At 40 days and 20 days before delivery, the sows were injected with immune inactivated vaccine and pig genetically engineered interferon once in each Houhai point. The grouping situation is shown in Table 4....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a porcine epidemic diarrhea virus strain, an inactivated vaccine and a vaccine composition of the inactivated vaccine and interferon, and belongs to the technical field of viruses and immunity. The Chinese epidemic diarrhea virus PEDV-CH-HB1801 strain adopted by the invention belongs to the current domestic epidemic GII subtype virus, the inactivated vaccine prepared from the strain has good immunogenicity and stability, and after pigs are inoculated with the inactivated vaccine, the organism can be stimulated to generate a high-level neutralizing antibody. In addition,after the inactivated strain and the porcine genetically engineered interferon are adopted for combined immunization, the immunogenicity is good, protective force can be generated for clinical epidemic strains, and practical and wide application value is achieved.

Description

technical field [0001] The invention belongs to the technical field of viruses and immunization, and in particular relates to a porcine epidemic diarrhea virus strain, an inactivated vaccine and a vaccine composition with interferon. Background technique [0002] Porcine epidemic diarrhea (PED) is an acute enteric disease caused by porcine epidemic diarrhea virus (PEDV) infection. PEDV can infect pigs of all ages, among which piglets under one week old are most seriously infected, mainly manifesting symptoms such as vomiting, watery diarrhea, and dehydration, with a fatality rate of over 90%. Necropsy of infected pigs showed that the villi of the jejunum and ileum shrank and fell off, and the intestinal wall became thin and transparent. Although the mortality rate of pigs over one week old is not high after infection, symptoms such as diarrhea, dehydration, and emaciation still occur, the feed return rate is significantly reduced, the resistance of pigs is reduced, and othe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/00A61K39/215A61K39/39A61P31/14A61K38/21A61K31/715
CPCC12N7/00A61K39/12A61P31/14A61K38/21A61K31/715A61K39/39C12N2770/20022C12N2770/20021A61K2039/5254A61K2039/552C12N2770/20034A61K2039/5252A61K2300/00
Inventor 梁婉周丹娜田永祥杨克礼郭锐袁芳艳刘泽文高婷刘威
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI