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Application of Celtis sinensis Pers. and extract thereof in gingko-related products

A technology of extract and hackberry, which is applied in the field of application of hackberry and its extracts in ginkgo-related products, can solve the problems that ginkgolic acid has not been systematically elucidated, and it is difficult to apply processing technology, so as to inhibit allergic reactions and reduce proliferation inhibition The effect of action

Pending Publication Date: 2021-03-23
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in the process of preparing EGB, it is necessary to control the residual ginkgolic acid in the product within the range of quality standards through processes such as separation and purification, but these methods are difficult to apply to the processing technology of ginkgo products such as ginkgo tea and ginkgo, and other methods need to be developed. method to ensure the safety of its consumption
So far, Ginkgo biloba is directly prepared into tea. During the brewing process, how much ginkgolic acid can be dissolved, whether it is harmful, and how to prevent and control it has not been systematically elaborated and effectively resolved.

Method used

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  • Application of Celtis sinensis Pers. and extract thereof in gingko-related products
  • Application of Celtis sinensis Pers. and extract thereof in gingko-related products
  • Application of Celtis sinensis Pers. and extract thereof in gingko-related products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Purpose of the experiment

[0027] To compare the amount of ginkgolic acid leached during brewing of different brands of ginkgo tea.

[0028] 2. Experimental materials

[0029] Ginkgo tea was purchased from four producing areas in Hubei, Jiangsu, Zhejiang and Shandong, among which the ginkgo tea in Hubei and Jiangsu was in the form of leaves, and the ginkgo tea in Zhejiang and Shandong was in the form of powdered tea bags; the standard products of total ginkgolic acid and ginkgo new acid were purchased in Nanjing King Bamboo Biotechnology Co., Ltd.

[0030] 3. Experimental protocol

[0031] Extraction method of total ginkgolic acid when brewing ginkgo tea: Take 15g of ginkgo tea from four different origins, weigh them precisely, put them into a thermos cup, add 200mL of 100°C boiling water to make tea, keep warm in a constant temperature water bath for 15min, filter , Collect tea water and tea dregs separately. Continue to add 200mL of boiling water at 100°C to t...

Embodiment 2

[0037] 1. Purpose of the experiment

[0038] To elucidate the hepatotoxicity and immunotoxicity of ginkgolic acid.

[0039] 2. Experimental materials

[0040] DMEM medium, RPMI 1640 medium, PBS phosphate buffer (Gibco); fetal bovine serum (Fetal BovineSerum, FBS, Hyclone); trypsin digestion solution (Beyond Biotechnology); trypan blue (Aladdin reagent); thiazole Blue (MTT, Sigma); human liver cancer cells HepG2 were purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences.

[0041] Clean-grade Balb / c mice, female, 6 weeks old, weighing 20±2g, were provided by the Comparative Medicine Center of Yangzhou University. Randomly divided into 2 groups, 8 in each group. They were divided into a control group (Sham.) and a ginkgolic acid experimental group (S.c.). They were raised at 21±2°C, with free access to food and water, and 12 hours of day and night alternation. The experimental operation of animals was strictly in accordance w...

Embodiment approach

[0043] 3.1 MTT method to detect the proliferation of HepG2 cells

[0044] HepG2 cells were cultured in DMEM (containing 10% FBS), 37°C, 5% CO 2 cultured under sterile conditions. Inoculate 4×10 per well in a 96-well plate 3 100 μL of HepG2 cells were cultured in DMEM complete medium at 37°C (5% CO 2 ), after the cells adhered to the wall for 6 hours, different concentrations of drugs were added to each well and cultured for another 72 hours. Add 20 μL of MTT (4 mg / mL) solution 4 hours before terminating the culture. After 4 hours, the supernatant was aspirated, and 200 μL of DMSO was added to each well, shaken, and the absorbance was detected at 540 nm, and the reading was recorded. Survival rate (%)=(ODcontrol-ODsample) / ODcontrol×100

[0045] 3.2 Mouse popliteal lymph node model

[0046] The two groups were subcutaneously injected on the right lower footpad of the mice on D0 respectively. The ginkgolic acid group was given 20 μL of 1 mg total ginkgolic acid, and the con...

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Abstract

The invention discloses application of Celtis sinensis Pers. and an extract thereof in gingko-related products. The extract of Celtis sinensis Pers. obtained through separation can relieve the anti-proliferative effect of ginkgolic acid on HepG2 cells, which can increase the cell survival rate to 63.0% from 26.7%; a good inhibition effect is achieved on T-cell-mediated inflammatory response, and the proliferation inhibition rate of Con A activated T cells can reach up to 95.4%; and the ethanol extract of Celtis sinensis Pers. leaves can effectively inhibit anaphylactic reactions caused by ginkgolic acid. The extract of Celtis sinensis Pers. or the Celtis sinensis Pers. leaves are directly added into products such as ginkgo tea and the like, so that the effects of toxicity reduction and efficacy enhancing can be effectively achieved. The invention provides a new method for safe application of products such as ginkgo tea and ginkgo containing ginkgolic acid, and provides a new solution for development of low-toxicity ginkgo tea products.

Description

technical field [0001] The invention relates to the field of health food, in particular to the application of hackberry and its extract in ginkgo-related products. Background technique [0002] Ginkgo biloba is rich in active substances such as flavonoids, terpene lactones, and polyprenyl alcohol. There are many teas or tea drinks made from ginkgo biloba on the market, which are harmful to the human body's cardiovascular, cerebrovascular, and nervous systems. It has certain health care effects, and is anti-fatigue and anti-aging, and is widely welcomed by modern consumers who pursue health. In addition to active substances, proteins, amino acids, minerals, vitamins and other nutrients, ginkgo tea also contains a certain amount of ginkgolic acid. Ginkgolic acid can cause severe inflammatory reactions, manifested in aspects such as liver damage and sensitization. Therefore, in the quality standards of ginkgo extract (EGB) at home and abroad, the main quality control indicato...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/34A61K36/185A61P29/00A61P1/16A61P17/00A61P37/08
CPCA23F3/34A61K36/185A61K36/16A61P29/00A61P1/16A61P17/00A61P37/08A61K2236/333A61K2236/39A61K2236/35A61K2236/33A61K2300/00
Inventor 赵林果房仙颖曹福亮汪贵斌苏二正
Owner NANJING FORESTRY UNIV
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