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Ultrahigh-density solid fermentation method of high-temperature-resistant bacillus

A technology of solid fermentation and bacillus, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve unsatisfactory problems, and achieve the effects of simple operation, prevention of excessive evaporation, and cheap raw materials

Pending Publication Date: 2021-03-26
ZHONGNONGXINKE SUZHOU ORGANIC RECYCLING RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional methods of landfilling, incineration, and pig feeding cannot meet people's requirements for health, sanitation, and resource recycling. Therefore, kitchen waste treatment equipment can be used for dehydration treatment, and then organic fertilizer can be produced through biological fermentation for agricultural use. production to achieve recyclable utilization of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Strain activation: LB broth 20g / L, agar 20g / L, pH 7, prepare LB solid medium, and sterilize. Take out the strain preservation tube stored in the -20°C refrigerator in advance, and wait until the ice is completely dissolved. Inoculate the bacteria into LB solid medium by streaking method in a sterile ultra-clean bench, and culture at 60°C for 20 hours to obtain activated bacteria for later use.

[0021] 2. Ultra-high density fermentation

[0022] (1) Preparation of liquid seed solution

[0023] Beef extract 3g / L, peptone 10g / L, NaCl 5g / L, pH=7, sterilized for later use. The activated strain was inoculated into 50 mL of liquid medium, cultured in a shaker at 180 r / min at 60°C for 3 days, and set aside.

[0024] (2) Preparation of solid fermentation medium

[0025] Take the required materials by weight percentage: 60 parts of bran, 25 parts of soybean meal, 10 parts of corn flour, and 5 parts of calcium carbonate. Rotate forward for 10 minutes, reverse for 10 minut...

Embodiment 2

[0029] 1. Strain activation: LB broth 20g / L, agar 25g / L, pH 7, prepare LB solid medium, and sterilize. Take out the preservation tube of the strain (ORI-1) stored in the -80°C refrigerator in advance, and wait until the ice is completely dissolved. Inoculate the strains to LB solid medium by streaking method in a sterile ultra-clean bench, and culture at 50°C for 24 hours to obtain activated strains for later use.

[0030] 2. Ultra-high density fermentation

[0031] (1) Preparation of liquid seed solution

[0032] Beef extract 3g / L, peptone 10g / L, NaCl 5g / L, ph=7, sterilized for later use. The activated strains were inoculated into 100 mL of liquid medium, cultured in a shaker at 180 r / min at 50°C for 5 days, and set aside.

[0033] (2) Preparation of solid medium

[0034] Weigh the required materials by weight percentage: bran 65%, soybean meal 25%, corn flour 7%, calcium carbonate 3%, all raw materials are placed in a dry mixer and fully mixed, the speed is 30r / min, Rot...

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PUM

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Abstract

The invention discloses an ultrahigh-density solid fermentation method of of high-temperature-resistant bacillus. The ultrahigh-density solid fermentation method comprises the following steps of (1) strain activation: culturing high-temperature-resistant bacillus in an LB solid culture medium to obtain an activated strain; (2) preparing a liquid seed solution, namely inoculating the activated strain obtained in the step (1) into a liquid LB culture medium for culturing; and (3) solid fermentation: inoculating the liquid seed solution prepared in the step (2) into a solid fermentation culture medium, and fermenting to obtain the high-density bacillus, wherein the fermentation culture medium comprises agar. According to the method, agar is added into an optimized solid culture medium as a water-retaining agent, so that ultrahigh-density solid fermentation of the high-temperature-resistant bacillus is realized, and the colony count of a final microbial agent product greatly exceeds the microbial agent standard and can reach 10 billion CFU / g.

Description

technical field [0001] The invention relates to the technical field of kitchen waste treatment, in particular to an ultra-high-density solid fermentation method of high-temperature-resistant bacillus. Background technique [0002] Kitchen waste mainly refers to the waste generated in the daily life of residents and activities such as food processing, catering services, and unit meals, including discarded leftovers, leftovers, peels, eggshells, bones, etc. With the improvement of residents' living standards and the rapid development of the catering industry, the output of kitchen waste in my country continues to grow. Traditional methods of landfilling, incineration, and pig feeding cannot meet people's requirements for health, sanitation, and resource recycling. Therefore, kitchen waste treatment equipment can be used for dehydration treatment, and then organic fertilizer can be produced through biological fermentation for agricultural use. production, to realize the recycli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20
Inventor 李季丁国春吴娴陶兴玲丁晓艳
Owner ZHONGNONGXINKE SUZHOU ORGANIC RECYCLING RES INST CO LTD
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