Method for extracting hpyerglycemic peptides from tea seed meal

A technology of tea seed meal and hypoglycemia, which is applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems affecting the application, and achieve the effects of low raw material, high inhibitory peptide activity, and wide application prospects

Pending Publication Date: 2021-03-26
DATUANJIE AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This will restrict the research on the mechanism of action of α-glucosidase inhibitory peptide derived from tea seed meal, and further affect its application in the development of hypoglycemic drugs

Method used

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  • Method for extracting hpyerglycemic peptides from tea seed meal
  • Method for extracting hpyerglycemic peptides from tea seed meal
  • Method for extracting hpyerglycemic peptides from tea seed meal

Examples

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Embodiment 1

[0025] Example 1: A method for extracting hypoglycemic peptides from tea seed meal

[0026] A method for extracting hypoglycemic peptides from tea seed dregs, comprising the following steps:

[0027] (1) Prepare tea seed meal powder. The tea seed meal is crushed with a pulverizer at a speed of 10000r / min. Every time the crush is 2 minutes, it is paused for 1 minute. After cumulative crushing for 4 minutes, the obtained powder is passed through a 60-mesh sieve, and the part under the sieve is the tea seed meal powder.

[0028] (2) Prepare defatted tea seed powder. Mix the tea seed meal powder with n-hexane at a ratio of 1:5, stir the mixture on a magnetic stirrer for 3 hours, remove the n-hexane by suction filtration, and dry the obtained powder at room temperature, which is defatted tea seed meal powder.

[0029] (3) Preparation of tea seed meal protein. Defatted tea seed meal powder and water were mixed at a mass ratio of 1:10 and the pH was adjusted to 10; the mixture was...

Embodiment 2

[0048] Example 2: A method for extracting hypoglycemic peptides from tea seed meal

[0049] (1) Prepare tea seed meal powder. The tea seed meal was crushed with a pulverizer at a speed of 10,000 r / min. Every time the crush was 2 minutes, it was paused for 1 minute. After cumulative crushing for 4 minutes, the obtained powder was passed through a 60-mesh sieve to obtain tea seed meal powder.

[0050] (2) Prepare defatted tea seed powder. Mix tea seed meal powder with n-hexane at a ratio of 1:5, stir the mixture on a magnetic stirrer for 3 hours (300r / min), remove n-hexane by suction filtration, and dry at room temperature to obtain defatted tea seed meal powder.

[0051] (3) Preparation of tea seed meal protein. Mix defatted tea seed meal powder and water at a ratio of 1:10 and adjust the pH to 10.0; the mixture is magnetically stirred at 55°C for 1.5 hours and centrifuged at 8000r / min for 15 minutes, and the obtained supernatant is adjusted to pH 4.5 with 1mol / L HCl , the s...

Embodiment 3

[0064] Example 3: A method for extracting hypoglycemic peptides from tea seed meal

[0065] A method for extracting hypoglycemic peptides from tea seed dregs, characterized in that it comprises the following steps:

[0066] Step 1: Pulverizing the tea seed meal to obtain tea seed meal powder;

[0067] Step 2: Mix the tea seed meal powder and n-hexane according to the mass ratio of 1:4, then stir the mixture, then remove the n-hexane by suction filtration, and dry the obtained powder at room temperature to obtain defatted tea seed meal powder;

[0068] Step 3: Mix defatted tea seed meal powder and water at a mass ratio of 1:7, then adjust the pH value to 9.5, heat the mixture to 45°C, then stir for 1, and then centrifuge at 7000r / min for 10min to obtain the above Adjust the pH value of the supernatant to 4.0, centrifuge at 7000r / min for 14min after 1h, resuspend the obtained precipitate with deionized water, and obtain the tea seed meal protein after freeze-drying;

[0069] S...

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Abstract

The invention discloses a method for extracting hypoglycemic peptides from tea seed meal. The method comprises the following specific steps: (1) crushing the tea seed meal; (2) degreasing the tea seedmeal; (3) carrying out tea seed meal protein extraction; (4) preparing tea seed meal crude peptides; (5) separating and screening alpha-glucosidase inhibitory peptides; and (6) carrying out structureidentification of the alpha-glucosidase inhibitory peptides. Through the steps, according to the invention, three alpha-glucosidase inhibitory peptides are prepared, the amino acid sequences of the alpha-glucosidase inhibitory peptides sequentially are a(GHSLESIK), b(GLTSLDRYK) and c(SPGYDGR), and the alpha-glucosidase inhibitory peptides have very high inhibitory activities on alpha-glucosidaseand have great application prospects in development of hypoglycemic products. The raw materials involved in the invention are cheap and wide in source, and the prepared alpha-glucosidase inhibitory peptides are high in activity and have wide application prospects.

Description

technical field [0001] The invention relates to a method for extracting a hypoglycemic peptide from tea seed dregs, and belongs to the technical field of intensive processing of agricultural products. Background technique [0002] In recent years, the number of people with diabetes in my country has been increasing. Drug treatment is the main means of controlling diabetes. For a long time, α-glucosidase inhibitors, insulin and some biguanide drugs are the most common hypoglycemic drugs in my country. Due to the relatively large side effects of these drugs or the need for extracorporeal injection, etc., the pain of the patient is also increased in the process of lowering blood sugar. Therefore, trying to use dietary therapy to control and treat diabetes has become a current research hotspot. Among them, dietary polypeptides with hypoglycemic effects have attracted much attention due to their wide range of sources and variety. [0003] Tea seed meal is the main by-product ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/36C07K1/34C07K1/30C07K1/16A61P3/10
CPCC12P21/06C07K1/145C07K1/36C07K1/34C07K1/30C07K1/16A61P3/10
Inventor 魏兆军马意龙冯俊张盛阳孙冬王玉平
Owner DATUANJIE AGRI CO LTD
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