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Lepidium meyenii glucosinolate extract and preparation method thereof

A technology of glucosinolate and glucosinolate is applied in the field of glucosinolate extract and preparation thereof, which can solve the problems of low glucosinolate content and low yield, and achieves good application prospect and high yield. , the effect of easy operation

Inactive Publication Date: 2021-03-30
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] It can be seen that there are problems such as low yield and low glucosinolate content in the extract currently extracted from cruciferous glucosinolate extracts.
At the same time, no glucosinolate extracts have been extracted from L.

Method used

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  • Lepidium meyenii glucosinolate extract and preparation method thereof
  • Lepidium meyenii glucosinolate extract and preparation method thereof
  • Lepidium meyenii glucosinolate extract and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Embodiment 1, the preparation of the glucosinolate extract in the unicycle of the present invention

[0042] Take 500g of lentils, crush them, add 5000ml of anhydrous methanol to extract under reflux at 75°C, the extraction time is 1 hour, take the supernatant after high-speed centrifugation of the extract, enrich the supernatant through a DEAE Sephadex A25 gel chromatography column, and use 2 Wash the impurities with sodium acetate buffer solution with a pH of 4 times column volume, add 2 times column volume concentration of 20 μg / ml sulfatase aqueous solution overnight, wash with water and dry to obtain glucosinolate extract. The yield of the glucosinolate extract is 5.2%, and the glucosinolate content in the glucosinolate extract is 54%.

Embodiment 2

[0043] Embodiment 2, the preparation of the glucosinolate extract in the unifoliate of the present invention

[0044]Take 1kg of lavender, crush it, add 15L of absolute ethanol to extract under reflux at 85°C, the extraction time is 2 hours, take the supernatant after the extract is centrifuged at high speed, and the supernatant is enriched by DEAE Sepharose CL-6B gel chromatographic column, After washing the impurities with 4 times column volume of sodium acetate buffer solution with pH 4, add 3 times column volume of sulfatase aqueous solution with a concentration of 20 μg / ml overnight, then wash with water and dry to obtain the glucosinolate extract. The yield of the glucosinolate extract is 5.4%, and the glucosinolate content in the glucosinolate extract is 55%.

Embodiment 3

[0045] Embodiment 3, the preparation of the glucosinolate extract in the unifoliate of the present invention

[0046] Take 10kg of lavender, crush it, add 20L of absolute ethanol to extract under reflux at 80°C, the extraction time is 3 hours, take the supernatant after the extract is centrifuged at high speed, and the supernatant is enriched by DEAE Sepharose CL-6B gel chromatographic column, Wash impurities with 3 times column volume of sodium acetate buffer solution with pH 4, add 1 times column volume of sulfatase aqueous solution with a concentration of 20 μg / ml overnight, rinse with water and dry to obtain glucosinolate extract. The yield of the glucosinolate extract is 5.5%, and the glucosinolate content in the glucosinolate extract is 54%.

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Abstract

The invention provides a lepidium meyenii glucosinolate extract and a preparation method thereof, and belongs to the technical field of medicines. The lettuce glucosinolate extract is a glucosinolateextract prepared by extracting cruciferous lettuce plants, wherein the total content of glucosinolate is greater than 50%. The invention also provides a method for extracting the thioglycoside extractfrom lepidium album. The extraction method is simple and convenient to operate and low in cost; compared with an existing method for extracting the thioglycoside extract from cruciferous plants, theyield of the thioglycoside extract extracted from lepidium album is high, the content of thioglycoside in the obtained thioglycoside extract is large, the content of thioglycoside in the thioglycosideextract is larger than 50% through the extraction method, and the method overcomes the problems of low yield and low content of the thioglycoside extract extracted from cruciferous plants in the prior art, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a glucosinolate extract of cephalocarpus and a preparation method thereof. Background technique [0002] The solitary cuisine is a perennial herb of the cruciferous family Cruciferae, which is distributed all over the world. In China, it is mainly produced in Inner Mongolia, Tibet, Qinghai and other places near the villages, fields, hillsides and salinized meadows at an altitude of 1800-4250m; the whole herb Used as medicine, it has the effects of clearing away heat and dampness, and treating bacillary dysentery and enteritis. [0003] Glucosinolates, referred to as glucosinolates, is an important secondary metabolite in cruciferous vegetables. According to the different side chain groups, glucosinolates can be divided into aliphatic, aromatic and indole Three categories of families. More than 120 kinds of glucosinolates have been found. There are about 15 specie...

Claims

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Application Information

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IPC IPC(8): A61K36/31A61P1/12A61P31/04A61P1/00A61P29/00
CPCA61K36/31A61P1/12A61P31/04A61P1/00A61P29/00A61K2236/33A61K2236/53A61K2236/55
Inventor 陈晨周国英李晶晶王博杨路存熊丰
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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