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A salt-tolerant Bacillus pumilus szg-ny-002 that can repair polluted water bodies and its composite bacterial agent and its application

A technology of Bacillus pumilus and composite bacterial agents, applied in the field of pollution control, can solve the problems of difficult repair technology and low efficiency of bioremediation methods, and achieve high COD degradation ability, strong adaptability, and accelerated degradation rate.

Active Publication Date: 2022-05-24
WUHAN SHUIZHIGUO ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a salt-tolerant Bacillus pumilus (Bacillus pumilus) SZG-NY-002 for remediation of high-salt polluted water in the environment, which is difficult and the efficiency of general bioremediation methods is low. , the preservation number of the strain is CCTCC NO: M 2020549

Method used

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  • A salt-tolerant Bacillus pumilus szg-ny-002 that can repair polluted water bodies and its composite bacterial agent and its application
  • A salt-tolerant Bacillus pumilus szg-ny-002 that can repair polluted water bodies and its composite bacterial agent and its application
  • A salt-tolerant Bacillus pumilus szg-ny-002 that can repair polluted water bodies and its composite bacterial agent and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Obtainment of Bacillus pumilus SZG-NY-001 and Bacillus pumilus SZG-NY-002:

[0026] Take fresh sludge from a salt lake in Hubei, and take 10 after dilution -4 , 10 -5 , 10 -6 Three gradients were applied, cultured at 37°C for one day, and a single colony was picked and inoculated on the slant.

[0027] Streaked culture with LB solid medium containing sodium chloride salt concentration of 5.0% (ie, 100 g of water with 5 g of sodium chloride), 10.0%, 15.0%, 20.0%, 25.0%, 30.0%, and cultured at 30°C for 3 days , observed its growth, selected 3 strains with better salt tolerance, and identified them, and finally identified two salt-tolerant Bacillus pumilus.

[0028] The two strains mentioned above have been sent to the China Center for Type Culture Collection for preservation on September 28, 2020. The classification name is: Bacillus pumilusSZG-NY-001, and the preservation number is CCTCC NO:M 2020550. Address: Wuhan University, Wuhan, China. Invention or called Bacil...

Embodiment 2

[0031] Fermentation culture of Bacillus pumilus SZG-NY-001 and Bacillus pumilus SZG-NY-002:

[0032] Shake flask medium: peptone 10g / L, yeast extract 5g / L, casein 5g / L, KCl 2g / L, sodium citrate 3g / L, MgSO 4 `7H 2 O 20g / L, NaCl 80g / L, Nystatin 50mg / L, pH 8.0, and the rest are water.

[0033] Use an inoculation loop to pick a single colony of Bacillus pumilus SZG-NY-001 or SZG-NY-002 on the plate, inoculate it in the above-mentioned medium, and shake it for 24 hours at a temperature of 30 ° C and 200 rpm / min to obtain a liquid inoculum. Seed liquid, the effective bacterial concentration of the seed liquid of the two bacteria is 1 × 10 8 cfu / mL.

Embodiment 3

[0035]Salt tolerance test of Bacillus pumilus SZG-NY-001 and SZG-NY-002:

[0036] Inoculate 5% Bacillus pumilus SZG-NY-001 or SZG-NY-002 seed solution in LB medium containing 0%, 12%, 14%, 16%, 18%, 20% of NaCl, After culturing for 24h, plate counting was performed, and the results were as follows:

[0037] Growth of Bacillus pumilus 001 in medium with different NaCl concentrations

[0038]

[0039]

[0040] The results show that Bacillus pumilus 001 has no inhibitory effect on the growth of the present invention when the NaCl concentration is not higher than 14%; When the concentration is 18%, the bacterial concentration is only 3 × 10 5 cfu / m. Therefore, it tolerates a maximum salinity of 16% sodium chloride.

[0041] Growth of Bacillus pumilus 002 in medium with different NaCl concentrations

[0042] NaCl concentration Bacterial concentration (cfu / mL) 0％ 1×10 8

[0043] The results show that Bacillus pumilus 002 has no inhibitory effect on the gr...

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Abstract

The present invention belongs to the technical field of pollution control, and specifically relates to a salt-tolerant Bacillus pumilus SZG‑NY‑002 that can repair polluted water bodies and its composite bacterial agent and its application. The preservation number of the Bacillus pumilus SZG‑NY‑002 is CCTCC NO: M2020549, this strain can treat high-salt industrial wastewater. After compounding it with Bacillus pumilus SZG‑NY‑001, the compound bacterial agent obtained has a higher degradation rate for high-salt industrial wastewater treatment, greatly improving Degradation effect of COD in high-salt wastewater.

Description

technical field [0001] The invention belongs to the technical field of pollution control, and in particular relates to a salt-tolerant Bacillus pumilus SZG-NY-002 capable of repairing polluted water bodies, a compound bacterial agent and application thereof. Background technique [0002] High-salinity wastewater refers to wastewater with a total salt content greater than or equal to 1%. This type of wastewater contains organic matter and at least 3.5% total dissolved solids in addition to large amounts of inorganic salts. It includes high-salt domestic wastewater and high-salt industrial wastewater, mainly from two sources: First, seawater is directly used in daily life and industrial production, such as seawater used for toilet flushing, road flushing and industrial cooling water, etc., resulting in discharge There are also a lot of inorganic salts in the wastewater; second, some industries such as seafood, dairy product processing, meat processing, printing and dyeing, pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07
CPCC12N1/20C02F3/34C02F3/341
Inventor 荆峰洋吴定心朱海孟希
Owner WUHAN SHUIZHIGUO ENVIRONMENTAL PROTECTION TECH CO LTD