Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Composition and application thereof in detecting activity of lipoprotein-associated phospholipase A2

A composition and lipoprotein technology, applied in the field of medical testing, can solve the problems of reduced substrate stability, inconvenient use, influence on reagent blank absorbance and reagent validity period, etc.

Active Publication Date: 2021-03-30
SHENZHEN AMTECH BIOENGINEERING LTD INC
View PDF10 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the substrate PAF analogs of this method are lipids, which are almost insoluble in water, and serum enzymes in the sample can also hydrolyze PAF analogs, making the determination results inaccurate
Although the use of ethanol or glycerin can promote the substrate to form a water phase, making the measurement results accurate, but the stability of the substrate is greatly reduced, and it is easy to degrade, which in turn affects the blank absorbance of the reagent and the validity period of the reagent.
Currently commercially available kits are mostly three reagents of R1, R2a, and R2b. R2a and R2b need to be mixed in proportion before use. The validity period of the mixed reagent is 28 days, which is inconvenient to use. The stability of the reagent after preparation is poor and the validity period is short.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition and application thereof in detecting activity of lipoprotein-associated phospholipase A2
  • Composition and application thereof in detecting activity of lipoprotein-associated phospholipase A2
  • Composition and application thereof in detecting activity of lipoprotein-associated phospholipase A2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The detection reagent combination of the present embodiment comprises:

[0066] The composition of reagent 1 (or called R1 reagent, the first reagent) is as follows:

[0067] 100mmol / L 4-hydroxyethylpiperazineethanesulfonic acid (HEPES), 15mmol / L ethylenediaminetetraacetic acid (EDTA), 9mmol / L 3-[3-(cholamidopropyl)dimethylaminopropanesulfonic acid Salt (CHAPS), 10mmol / L sodium n-nonane sulfonate, 200mmol / L sodium chloride, the balance is water; the pH of reagent 1 is 7.6.

[0068] The composition of reagent 2 (or called R2 reagent, second reagent) is as follows:

[0069] 20mmol / L citric acid, 15mmol / L sodium n-nonanesulfonate, 5wt% ethanol, 3mmol / L 1-tetradecanoyl-2-4-p-nitrophenol succinic anhydride-3-phosphatidylcholyl, 0.05wt %18-Crown Ether-6, 0.1wt% Dextran 40 (also known as Dextran-40, Dextran-40, Dextran T-40, Dextran T-40), the balance is water; the pH of reagent 2 is 4.5 . The mass percent concentration mentioned in Reagent 2 refers to the mass percent con...

Embodiment 2

[0071] Lp-PLA in embodiment 2 and embodiment 1 2 The only difference in the assay kit is that the stabilizer in reagent R2 is 0.05wt% 18-crown-6, and the others are the same as those in Example 1, and will not be repeated here.

Embodiment 3

[0073] Embodiment 3 and the Lp-PLA in embodiment 1 2 The only difference in the assay kit is that the stabilizers in the reagent R2 are 0.05wt% 15-crown-5 and 0.1wt% dextran 40. Others are the same as in Example 1 and will not be repeated here.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a composition and an application thereof in detecting the activity of lipoprotein-associated phospholipase A2, wherein the composition contains a substrate and a stabilizer; the stabilizer is selected from at least one of crown ether compounds and glucan compounds, the substrate can bind to the lipoprotein-associated phospholipase A2, and the substrate bound to the lipoprotein-associated phospholipase A2 can be decomposed. By adding the stabilizer, the substrate is more stable and is not easy to hydrolyze, and the blank absorbance of a reagent is effectively controlled,so that the validity period of the reagent reaches 12 months or more.

Description

technical field [0001] The invention relates to the technical field of medical testing. In particular, it relates to a composition and its application in detecting the activity of lipoprotein-associated phospholipase A2. Background technique [0002] Studies have shown that the prevalence of cardiovascular disease in China and other countries is on the rise. Studies have shown that atherosclerosis is the pathological basis of cardiovascular and cerebrovascular diseases. [0003] Lipoprotein-associated phospholipase A2 (Lp-PLA for short) 2 ) can generate lysophosphatidylcholine (lysophosphatidylcholine, Lyso-PC) and oxidized free fatty acids (oxidized free fatty acids, ox-FA) by hydrolyzing oxidized phospholipids on the oxidized low-density lipoprotein (LDL) on the arterial intima, These two pro-inflammatory mediators can stimulate the production of adhesion factors and cytokines, promote the occurrence and development of atherosclerosis, and lead to thrombosis and cardiov...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/44
CPCC12Q1/44C12Q2334/10G01N2333/918
Inventor 陈小茹吴传侠吴向东周博
Owner SHENZHEN AMTECH BIOENGINEERING LTD INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com