Controllable gradient stent loaded with drugs, active factors and cells, 3D printing method of controllable gradient stent and special multi-nozzle 3D printer

Abstract

The invention relates to the technical field of biological tissue and organ 3D printing, in particular to a controllable gradient stent loaded with drugs, active factors and cells, a 3D printing method of the controllable gradient stent and a multi-nozzle 3D printer. The controllable gradient stent is formed through one-step alternate printing; the integrated gradient stent at least comprises twomaterials, namely a first material and a second material, wherein the first material is loaded with one or more of the drugs, the active factors or the cells; the second material is loaded with one ormore of the drugs, the active factors or the cells; and the first material and the second material are alternately printed to construct the integrated gradient stent. The integrated gradient stent constructed by the invention and loaded with the drugs, the active factors or the cells does not have the problems that a double-layer gradient interface is not firm and easy to fall off. The integratedgradient stent of the three-dimensional cells is constructed in a multi-nozzle alternate printing manner, and a foundation is provided for construction of complex tissues and organs of organisms.

Description

technical field [0001] This application relates to the technical field of 3D printing of biological tissues and organs, in particular to a controllable gradient scaffold loaded with drugs, active factors and cells, its 3D printing method and a multi-nozzle 3D printer. Background technique [0002] For a long time, researchers have tried to construct complex tissues and organs in the body through tissue engineering methods, so as to solve the problem of repairing damaged tissues in clinical practice and many problems such as infection, rejection, and donor shortage faced by organ transplantation. However, the functional microenvironment of different tissues and organs is quite different. Therefore, it is of great significance to choose the appropriate technology to successfully construct tissues and organs with differential functionalization in vitro, realize the gradient scaffold that can load cells and can simultaneously regulate the microenvironment to promote repair and re...

AI Technical Summary

Problems solved by technology

[0004] Although the first type has a more precise structural design, the scaffold itself does not contain cells. After implanting the defect site, the cells need to be guided to grow into the scaffold, which may easily lead to uneven growth of cells in the scaffold and "hollowing" phenomenon, or The constructed scaffold has large pores and it is difficult to obtain micropores below 100 μm, so that it cannot provide a three-dimensional support environment conducive to cell growth, so it is not easy for cells to adhere during culture

[0005] Although the second type introduces exogenous cells, the formed scaffold still has the problem of poor structural controllability and the inability to achieve precise structures.

[0006] The third type of current 3D printing technology printing method is relatively simple, commonly used single-nozzle printing method, which can only print brackets constructed with a single material

When the local structure of the two kinds of scaffolds is required to be combined, the problem that the double-layer gradient interface is not firm and easy to fall off is likely to occur

Therefore, it is impossible to realize the construction of complex tissues and organs

[0007] Therefore, in view of the above-mentioned related technologies, the inventor believes that there is a defect in the current technology that it is impossible to provide a controllable gradient scaffold with high precision and bionic complex tissues and organs

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