Method for extracting apigenin from Ostericum sieboldii and application

An apigenin and extraction technology, which is applied to medical preparations, pharmaceutical formulations, plant raw materials and other directions containing active ingredients, can solve the problems of inability to excrete uric acid normally, low excretion, and undisclosed apigenin, and optimize the extraction sequence. , The effect of reducing uric acid, improving yield and purity

Active Publication Date: 2021-04-02
吉林浩泰健康产业发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are four main reasons for the accumulation of uric acid: the first is excessive consumption of high-purine foods; the second is a problem with purine metabolism in the body; t

Method used

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  • Method for extracting apigenin from Ostericum sieboldii and application
  • Method for extracting apigenin from Ostericum sieboldii and application
  • Method for extracting apigenin from Ostericum sieboldii and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A method for extracting apigenin from celery, comprising the steps of:

[0053] (1) Dry and pulverize the celery, add 5 times the amount of solvent A to extract, add 3% xylanase for enzymolysis for 10 minutes, and then add 5% glucosidase, the solvent A is ethylene glycol, iso A mixed solvent of propanol and water, the volume ratio of the three is 2:1:1, the extraction temperature is 30°C, the total extraction time is 1h, and the extract is concentrated to 1 / 5 of the original volume to obtain substance A;

[0054] (2) Substance A is successively added into equal volumes of petroleum ether, chloroform and ethyl acetate for extraction, the extraction layers of chloroform and ethyl acetate are collected, dried respectively, and combined to obtain a crude product;

[0055] (3) The crude product is dissolved with 30% ethanol of 3 times the amount, and the D101 macroporous adsorption resin is used for adsorption, and the solvent D is used for elution, and the solvent D is a mi...

Embodiment 2

[0058] A method for extracting apigenin from celery, comprising the steps of:

[0059] (1) Dry and pulverize the celery, add 10 times the amount of solvent A to extract, add 8% xylanase for enzymolysis for 25min, then add 10% glucosidase, the solvent A is ethylene glycol, iso A mixed solvent of propanol and water, the volume ratio of the three is 4:1:3, the extraction temperature is 40°C, the total extraction time is 2h, and the extract is concentrated to 1 / 8 of the original volume to obtain substance A;

[0060] (2) Substance A is successively added into equal volumes of petroleum ether, chloroform and ethyl acetate for extraction, the extraction layers of chloroform and ethyl acetate are collected, dried respectively, and combined to obtain a crude product;

[0061] (3) The crude product is dissolved with 5 times of 90% ethanol, adsorbed by D101 macroporous adsorption resin, and eluted by solvent D, which is a mixed solvent of chloroform and methanol, and the volume ratio of...

Embodiment 3

[0064] A method for extracting apigenin from celery, comprising the steps of:

[0065] (1) Dry and pulverize the celery, add 8 times the amount of solvent A to extract, add 5% xylanase for enzymolysis for 15min, then add 7% glucosidase, the solvent A is ethylene glycol, iso A mixed solvent of propanol and water, the volume ratio of the three is 3:1:2, the extraction temperature is 35°C, the total extraction time is 1.5h, and the extract is concentrated to 1 / 6 of the original volume to obtain substance A;

[0066] (2) Substance A is successively added into equal volumes of petroleum ether, chloroform and ethyl acetate for extraction, the extraction layers of chloroform and ethyl acetate are collected, dried respectively, and combined to obtain a crude product;

[0067] (3) The crude product is dissolved with 4 times of 60% ethanol, adsorbed by D101 macroporous adsorption resin, and eluted by solvent D, which is a mixed solvent of chloroform and methanol, and the volume ratio of...

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Abstract

The invention relates to the technical field of plant extraction, and particularly relates to a method for extracting apigenin from Ostericum sieboldii and application. The method comprises the stepsof (1) drying and smashing Ostericum sieboldii, adding a solvent A for extraction, and concentrating an extracting solution to obtain a substance A; (2) extracting the substance A with a solvent B, and drying an extracted solvent B layer to obtain a crude product; (3) dissolving the crude product with a solvent C, adsorbing with macroporous adsorption resin, and eluting with a solvent D to obtainan eluent; and (4) concentrating and crystallizing the eluent to obtain the product. The method has the advantages that the yield and the purity are high, and the effect of reducing uric acid is remarkable by combining the apigenin with other effective components for use.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a method for extracting apigenin from celery and its application. Background technique [0002] Celery is a perennial herb of Umbelliferae and the genus Celery. Celery has both medicinal and edible value. Shanqin has the effects of mainly treating rheumatic arthralgia, soreness of the waist and knees, cold headache, carbuncle sores, swelling and pain. Celery is a high-grade rare vegetable in celery. The nutrient content of wild celery is also higher in wild vegetables, and the seedlings of wild celery can be used as wild vegetables in spring. Its nutritional effects are as follows: (1) Celery has high iron content, which can supplement the loss of women's menstrual blood. Eating it can prevent pale, dry and dull complexion, and can make eyes bright and hair black and shiny. (2) Celery is a high-fiber food. It produces a lignin or intestinal fat substance through intes...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40A61K31/352A61K31/519A61P19/06A61K36/238A61K31/353A61K31/37
CPCA61K31/352A61K31/353A61K31/37A61K31/519A61K36/238A61K2236/19A61K2236/333A61K2236/55A61P19/06C07D311/30C07D311/40A61K2300/00
Inventor 李雪殷金龙殷金玲董志杰
Owner 吉林浩泰健康产业发展股份有限公司
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