Broad-spectrum antifungal bacillus altitudinis ZKY02 and application thereof

A technology of Geobacillus and Bacillus, applied to the broad-spectrum antifungal Bacillus highlander ZKY02 and its application field, which can solve the problems of preventing and controlling fruit rot with Bacillus highlander that has not been seen yet

Active Publication Date: 2021-04-02
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on biological control of peanut fruit rot disease prevention and control has just started in China. There are only research reports on the use of Bacillus amyloliquefaciens to prevent and control fruit rot disease, and there are no reports on the use of Bacillus uplander to prevent and control fruit rot disease

Method used

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  • Broad-spectrum antifungal bacillus altitudinis ZKY02 and application thereof
  • Broad-spectrum antifungal bacillus altitudinis ZKY02 and application thereof
  • Broad-spectrum antifungal bacillus altitudinis ZKY02 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Separation, purification and physicochemical properties of ZKY02

[0028] (1) Isolation, purification and preservation of ZKY02

[0029] In the Dachikan Banana Plantation in Doumen District, Zhuhai City, the rhizosphere soil of healthy Brazilian Banana in the diseased place was collected, and the bacteria were isolated and cultured on LB by the dilution plate method; the bacteria ZKY02 with different shapes were picked and grown at a constant temperature of 37°C overnight. , purified and cultured on a new LB plate, and stored at -20°C and -80°C with 30% glycerol in the form of bacterial liquid.

[0030] (2) Determination of physical and chemical properties of ZKY02

[0031] For the test method and required culture medium, refer to the "Common Bacteria System Identification Manual" (edited by Dong Xiuzhu, Cai Miaoying, etc., Beijing: Science Press, 2001), and the identification of biochemical indicators uses the common bacteria identification tube of Guangzho...

Embodiment 2

[0042] Example 2: Detection of antagonistic activity of ZKY02 to different physiological races of banana Fusarium wilt

[0043] The broad-spectrum antagonistic activity of ZKY02 against banana Fusarium wilt was detected by using the banana Fusarium wilt strains preserved in the laboratory. Fusarium oxysporum, FOC004 and 005 are physiological races No. 1 of Banana Fusarium wilt, and other strains are Physiological race No. 4 of Banana Fusarium wilt. FOC007 strain was isolated from Zhanjiang, and other strains were isolated from different banana planting places in Zhuhai (Cao Yongjun et al., using ITS1 and ITS4 universal primers amplify the nucleic acid fragment of Fusarium wilt of banana and identify its physiological race, Journal of Tropical Crops, 2010,31(7):1098-1102).

[0044] 1. Strain activation: Scrape a small amount of SNA-preserved pathogenic bacteria and inoculate them on a PDA plate, incubate at 27°C for 4-5 days, then use a sterilized 1mL plastic tip to punch a hol...

Embodiment 3

[0053] Example 3, ZKY02 is tested for the antagonistic activity of other Fusarium fungi that cause root rot and withering of crops

[0054] The method for bioassaying antagonistic activity is the same as in Example 2, wherein the pathogenic bacteria for testing include the pathogenic bacteria Fusarium oxysporum celery specialization type 2 physiological race FOA strains that cause Verticillium wilt of celery, and the Fusarium graminearum that causes corn root rot F.g. strains and Fusarium pseudoverticillium F.v., Fusarium oxysporum watermelon specialization 1 physiological race causing wilt of watermelon, Fusarium oxysporum cucumber specialization causing cucumber wilt, Solanum oxysporum causing cucumber root rot disease Fusarium. The measurement results are shown in Table 2. ZKY02 has broad-spectrum antagonistic activity against Fusarium diseases from celery, corn, watermelon and cucumber.

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Abstract

The invention discloses broad-spectrum antifungal bacillus altitudinis ZKY02 and application thereof. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on December 2, 2019, and the address is the fifth floor of the 59# building of the 100# courtyard of the Xianlie Middle Road of Guangzhou City; and the preservation number of the strain is GDMCC NO: 60910. According to the application of the bacillus altitudinis ZKY02 in preparation of a preparation for preventing and treating phytopathogens, the strain has good plate antagonistic activity on a variety of plant pathogenic fungi, and especially has good antagonistic ability on soil-borne vascular bundle diseases caused by a variety of fusarium.

Description

technical field [0001] The invention belongs to the fields of biological pesticides and disease-resistant microbial fertilizers, and relates to a strain of broad-spectrum anti-fungal bacillus highlander ZKY02 and its application. Background technique [0002] Vascular bundle disease, a soil-borne disease caused by Fusarium fungus, is one of the most important diseases in agricultural production, seriously affecting the healthy development of agriculture. Among them, Fusarium oxysporum has more than 120 host types, and each host type is divided into several physiological races, and some physiological races also have differences in geographical strains. The diversity of pathogenicity and genetic characteristics of pathogenic fungi has brought great difficulties to the prevention and control. Take banana fusarium wilt as an example. The disease is caused by Fusarium oxysporumf.sp.cubense. There are 3 physiological races, among which No. 1 and No. 4 physiological races are harm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/22Y02A40/10
Inventor 喻国辉程萍李春季郑丽孙昀皓
Owner ZHONGKAI UNIV OF AGRI & ENG
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