Culture medium supplementing composition, stem cell culture medium and preparation method of stem cell culture medium

Abstract

The invention relates to a culture medium supplementing composition, a stem cell culture medium and a preparation method of the stem cell culture medium. In stem cell research, the inventor finds that reduced glutathione and vitamins, in particular vitamin E or / and vitamin E analogues, have the effect of maintaining stem cell proliferation ability and stemness, and when the culture medium supplementing composition containing reduced glutathione and vitamin E or / and vitamin E analogues is added to a basal culture medium, the stem cells actively cultured by the obtained culture medium can effectively maintain the proliferation ability and the undifferentiated state of the stem cells; meanwhile, the oxidative stress reaction of the stem cells is also reduced.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a culture medium supplement composition, a stem cell culture medium and a preparation method thereof. Background technique [0002] Embryonic stem cells (ESCs) are cell groups with a high degree of differentiation ability, which can be differentiated into different types of functional cells. Maintaining good stemness of embryonic stem cells in vitro is a key factor for inducing differentiation, constructing disease models, and drug screening tools. However, when embryonic stem cells are cultured in vitro, the cells will be affected by the culture environment, medium components, and the level of experimental operators. In particular, when embryonic stem cells are continuously subcultured for a long time, if they cannot be maintained in a good culture environment, the cells will be subjected to different stimuli, resulting in poor cell state, decreased stemness, and uncontrol...

AI Technical Summary

Problems solved by technology

However, when embryonic stem cells are cultured in vitro, the cells will be affected by the culture environment, medium components, and the level of experimental operators.

In particular, when embryonic stem cells are continuously subcultured for a long time, if they cannot be maintained in a good culture environment, the cells will be subjected to different stimuli, resulting in poor cell state, decreased stemness, and uncontrollable differentiation ability.

[0003] The presence of oxidative stress (OS) often requires an adaptive response of endogenous anti-oxidative stress mechanisms, which in turn significantly regulates the level of oxidative stress, leading to increased oxidative stress response

On the one hand, it increases the level of eNOS (nitric oxide synthase), leading to decreased activity of α-ketoglutamate dehydrogenase, mitochondrial dysfunction, and increased reactive oxygen species (ROS) levels

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