Cotton verticillium wilt disease phenotype identification method

Abstract

The invention discloses a cotton verticillium wilt disease phenotype recognition method. The method comprises the following steps of 1, cultivating cotton seedlings in a non-woven fabric seedling raising bag; step 2, acquiring a canopy spectrum curve by utilizing a near-infrared multispectral technology; step 3, analyzing sensitive waveband correlation among different resistant varieties; step 4, establishing an estimation model according to the waveband combination; and step 5, obtaining a sensitive waveband corresponding to the disease grade. The method disclosed by the invention is low in destructiveness, simple to operate, labor-saving, high in inoculation success rate, uniform and consistent in morbidity and suitable for carrying out large-batch material identification work on vast scientific research workers, the bottoms of the nutrition pots do not need to be cut off, and the accuracy is higher than that in the prior art.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and relates to a method for identifying the occurrence phenotype of cotton verticillium wilt disease, in particular to a rapid phenotype identification method for evaluating the occurrence severity of cotton verticillium wilt disease by using near-infrared multi-spectral technology. Background technique [0002] Xinjiang has become the largest cotton production base in my country. The total cotton production accounts for more than 80% of the country's total. Xinjiang's cotton production is directly related to the country's cotton supply and food security. Verticillium wilt is one of the seven major soil-borne diseases in the world. It has the characteristics of wide distribution, serious damage and strong infection. It is extremely difficult to cure and is called "cotton cancer". At present, it is recognized internationally that planting disease-resistant varieties is the most economical and ...

AI Technical Summary

Problems solved by technology

The method of directly watering the roots with the bacterial solution is slow and uneven

Wounded root inoculation is the most commonly used method. This method has a fast onset and relatively accurate identification results, but it requires high technical requirements, and the steps are cumbersome, time-consuming and labor-intensive.

At present, the common identification method of cotton Verticillium wilt resistance in China—the disease index method (Cotton pest resistance evaluation technical specification part 5: Verticillium wilt, GB / T22101.52009; A kind of cotton Verticillium wilt resistance seedling The identification method, CN201110081889.7), is based on the comprehensive judgment of the incidence and severity of the leaves, but if hidden symptoms appear in the cotton plants, the application of the disease index method has relatively large limitations; the cotton seedlings can be judged according to the activity of the relevant enzymes after inoculation. In the method of disease resistance (a detection method of cotton verticillium wilt disease resistance, application number: CN201510111867.9), the onset of cotton seedlings can be detected without obvious symptoms, and the measurement results have certain accuracy, but the enzyme The activity determination process is complicated and time-consuming, and it is not suitable for the rapid identification of Verticillium wilt resistance in batches

[0004] It can be seen that the existing identification technology has a large investment in manpower, material resources and financial resources, which is not suitable for batch screening, and there are certain human errors in the identification results.

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