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Indirect immunofluorescence kit for detecting epidemic QX chicken infectious bronchitis virus antibody based on S1 protein

A chicken infectious and immunofluorescence technique used in the field of indirect immunofluorescence kits

Inactive Publication Date: 2021-04-09
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no rapid method and kit for QX type IBV serological antibody detection at present

Method used

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  • Indirect immunofluorescence kit for detecting epidemic QX chicken infectious bronchitis virus antibody based on S1 protein
  • Indirect immunofluorescence kit for detecting epidemic QX chicken infectious bronchitis virus antibody based on S1 protein
  • Indirect immunofluorescence kit for detecting epidemic QX chicken infectious bronchitis virus antibody based on S1 protein

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Embodiment Construction

[0043] The present invention will be further described below in conjunction with the drawings and descriptions of the drawings.

[0044] 1, the isolation of QX type infectious bronchial virus: get the trachea and kidney of the dead chicken of suspected QX type infectious bronchial virus infection, tissue sample is made into 10%w with PBS (containing 10000M penicillin and 100 μ g streptomycin pH7.2) / v homogenate, 4°C. Centrifuge at 5000r / min for 10min, take the supernatant and place it at 37°C for 20min. The strain was diluted 100 times with sterilized PBS. Inoculate 9-11-day-old SPF chicken embryos into the allantoic cavity, 0.2 mL / embryo. After inoculation, place in a constant temperature incubator at 37°C for 120 hours. The chicken embryos that died within 24 hours were discarded, the allantoic fluid of live embryos was aseptically harvested, and the growth and development of the chicken embryos were observed. The germ fluid that has been tested as sterile and negative ...

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Abstract

The invention discloses an indirect immunofluorescence kit for detecting an epidemic QX chicken infectious bronchitis virus antibody based on S1 protein. The kit comprises LMH cells for expressing the S1 protein, an FITC labeled goat anti-chicken antibody, a sample diluent and a cleaning solution. The kit disclosed by the invention has good specificity; the kit not only can be used for epidemiological investigation of QX-type chicken infectious bronchitis virus infection conditions, but also can be effectively used for monitoring the antibody level of the QX-type chicken infectious bronchitis virus of immune chicken flocks, evaluating the immune protective antibody level and the like.

Description

technical field [0001] The invention relates to an indirect immunofluorescence reagent kit for detecting prevalent QX type chicken infectious bronchial virus antibody based on S1 protein, which belongs to the field of biotechnology detection. Background technique [0002] Infectious bronchitis virus (IBV) belongs to the Coronaviridae family Coronaviridae), the genus Coronaviridae, and can be divided into respiratory type, genital tract type, kidney type, intestinal type, glandular stomach type and mutant type according to clinical conditions. The disease is mainly characterized by coughing, tracheal rales and sneezing. It is one of the most serious viral infectious diseases to the poultry industry in my country. The disease exists in almost all poultry-raising countries in the world. Upward trend. There are many serotypes of IBV and they mutate rapidly. The natural recombination between different strains of IBV can produce new strains, which in turn leads to the emergence of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569C12N15/50C12N15/85C12N5/10C07K14/165
CPCC07K14/005C12N15/85C12N2770/20022G01N33/56983G01N2333/165G01N2469/20
Inventor 叶建强路浩尹朝憬武佳妍谢菁张建军邵红霞秦爱建
Owner YANGZHOU UNIV