Method for quickly preparing [beta]-nicotinamide mononucleotide by enzymic method

A single nucleotide and nicotinamide technology, applied in the biological field, can solve problems such as poor prospects for industrial production, complicated NR chemical synthesis, and slow reaction speed, and achieve stable and rapid synthesis process, huge price advantage, and fast reaction speed Effect
CN112646851AActive Publication Date: 2021-04-13ANHUI GSH BIO TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ANHUI GSH BIO TECH CO LTD
Publication Date
2021-04-13

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Abstract

The invention discloses a method for quickly preparing [beta]-nicotinamide mononucleotide by an enzymic method. The method comprises the following steps of: taking PRPP as a raw material, and on the basis, adding nicotinic acid and / or quinolinic acid, ammonium ions, ATP and magnesium ions and / or manganese ions to form a reaction system; and under catalysis of an EC 6.3.4.21 enzyme and an EC 6.3.1.5 enzyme, or under the catalysis of an EC 2.4.2.19 enzyme and an EC 6.3.1.5 enzyme or under the catalysis of the EC 6.3.4.21 enzyme, the EC 6.3.1.5 enzyme and the EC 2.4.2.19 enzyme, enabling the reaction system to react to quickly prepare the [beta]-nicotinamide mononucleotide. Compared with a chemical method and a mixing method, the method disclosed by the invention has a stable and quick synthesis technology, no pollutants, including organic solvents and the like, are added in a whole process, and the method disclosed by the invention is environmentally friendly and is safe in production. Compared with other enzymic method production methods, the method disclosed by the invention has the advantages of a high reaction speed, a high conversion rate, a low production cost and a wide substrate range.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for rapidly preparing β-nicotinamide mononucleotide by enzymatic method. Background technique

[0002] β-nicotinamide mononucleotide, referred to as NMN, has a molecular weight of 334.22. Studies have shown that it plays an important role in human cell repair and anti-aging. In recent years, journals such as Science, Nature, and Cell have published a number of articles on the efficacy of NMN. Harvard University experiments have confirmed that supplementing NMN can extend the lifespan of mammals by more than 30%. Therefore, NMN is regarded as an "elixir of life". , highly sought after by the health care products and pharmaceuticals market. However, the current market price of NMN is on the high side. The cheapest domestic product is about 1,500 yuan per 9,000 mg, and the price of imported products is higher, which cannot really benefit ordinary people.

[0003] Existing NM...

Claims

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