Stem tip culture and rapid propagation method of pueraria lobata var. thomsonii
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A culture medium formula and technology of powdered kudzu, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of seed stems with pathogens, low reproduction coefficient, and irregular seedlings, etc., to increase income, improve The effect of reproduction coefficient and uniform growth
Pending Publication Date: 2021-04-16
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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[0004] The purpose of the present invention is to provide a method for rapid propagation of kudzu stem tip culture, which solves the problems of high cost of reproduction by using adult stems with high medicinal value, irregular seedlings and stems carrying pathogens, and low reproduction coefficient for the existing kudzu. lack of
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Embodiment 1
[0039] A kind of rapid propagation method of kudzu root tip culture, comprising the following steps:
[0040] (1) Explant selection and disinfection:
[0041] ①Select the young and tender shoots of Pueraria puerariae growing healthy as explants,
[0042] ② Wash the branches with a neutral detergent to remove the dirt on the surface of the branches, then remove the excess leaves, and dry the surface with a clean paper towel.
[0043] ③ On the ultra-clean workbench, first soak it with 70% alcohol for 20 seconds, then rinse it with sterile water for 3 times, each time for 4 minutes,
[0044] ④ Immediately transfer to 0.2% mercuric chloride solution for disinfection for 10 minutes, and finally wash with sterile water 4 times, each time for 5 minutes, to obtain sterilized explants;
[0045] (2) Preparation of improved MS medium:
[0046] The formula of the improved MS medium is: the preparation of the improved MS medium, the formula is: add polyvinylpyrrolidone (PVP) to the MS m...
Embodiment 2
[0057] A method for rapid propagation of Nansha kudzu root tip culture, comprising the following steps:
[0058] (1) Explant selection and disinfection:
[0059] ①Select the young and tender shoots of Pueraria puerariae growing healthy as explants,
[0060] ② Wash the branches with a neutral detergent to remove the dirt on the surface of the branches, then remove the excess leaves, and dry the surface with a clean paper towel.
[0061] ③ On the ultra-clean workbench, first soak it with 70% alcohol for 25 seconds, then rinse it with sterile water for 3 times, each time for 3 minutes,
[0062] ④ Immediately transfer to 0.25% mercuric chloride solution for disinfection for 10 minutes, and finally wash with sterile water for 4 times, each time for 5 minutes, to obtain sterilized explants;
[0063] (2) Preparation of improved MS medium:
[0064] The formula of the improved MS medium is: the preparation of the improved MS medium, the formula is: add polyvinylpyrrolidone (PVP) to ...
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Abstract
The invention provides a stem tip culture and rapid propagation method of pueraria lobata var. thomsonii. The method comprises the following steps: (1) selection and disinfection of explants; (2) preparation of culture medium; (3) separation of stem tips and induction culture of adventitious buds: inoculating stem tips of the disinfected explants into an adventitious bud induction culture medium for induction culture to obtain adventitious buds of pueraria lobata var. thomsonii; (4) subculture multiplication: inoculating the adventitious buds of the pueraria lobata var. thomsonii into a subculture multiplication culture medium for subculture multiplication culture to obtain aseptic seedlings of the pueraria lobata var. thomsonii; (5) sterile rooting: cutting and propagating the sterile seedlings of the pueraria lobata var. thomsonii, inoculating the sterile seedlings into a sterile rooting culture medium for rooting culture to obtain young seedlings of the pueraria lobata var. thomsonii, and hardening and transplanting the seedlings; and (6) identification of virus-free seedlings: taking plant leaves for RNA identification and selecting a strain with a corresponding sequence of virus-free nucleic acid as a healthy seedling for expanding propagation. The stem tip culture and rapid propagation method of pueraria lobata var. thomsonii can rapidly culture seedlings of pueraria lobata var. thomsonii without aggregation of pathogens, improves a propagation coefficient and also has a short culture period.
Description
technical field [0001] The invention belongs to the technical field of rapid plant propagation, and in particular relates to a rapid propagation method for kudzu root tip culture. Background technique [0002] Fenge (Puerariathomsonii Benth.) is a perennial vine of the genus Pueraria in the Fabaceae family. It is a traditional Chinese herbal medicine that relieves heat and cools the body. It has the reputation of "Southern Ginseng". Pueraria kudzu is widely distributed and has a long history of planting in most parts of my country. Some kudzu kudzu has also obtained national geographical indications, and is an important plant with the same origin of medicine and food. Pueraria japonica has the functions of relieving muscle and reducing fever, dredging meridians and activating collaterals, and relieving alcohol poisoning. Among them, kudzu powder is used as the main raw material for Sangge Jiangzhi Pills, Fenge Decoction Tablets, Fenge Decoction Granules, Fengeqinlian Pills a...
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