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Chitosan/hectorite composite hydrogel for 3D printing and preparation method and application thereof

A composite hydrogel, 3D printing technology, applied in the field of 3D printing and biomedical materials, can solve the problems of high hectorite content, harm, cytotoxicity, etc.

Active Publication Date: 2021-04-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high content of hectorite used in the current relevant research is likely to cause cytotoxicity and potential harm

Method used

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  • Chitosan/hectorite composite hydrogel for 3D printing and preparation method and application thereof
  • Chitosan/hectorite composite hydrogel for 3D printing and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Dissolve 5g of hydroxymethyl chitosan powder in 200ml of 0.01mol / L phosphate buffer solution, stir and heat to 50°C, then add methacrylic anhydride dropwise under sealed conditions (the volume of methacrylic anhydride is equal to phosphoric acid 0.25% of the volume of the salt buffer solution), after reacting for 4 hours, the solution was dialyzed for 5 days, and then freeze-dried to obtain methacrylated chitosan derivatives (methacrylated hydroxymethyl chitosan);

[0035] The product that photoinitiator 2959, hectorite, step (1) obtains is dissolved in pure water successively (the massfraction of photoinitiator 2959, hectorite, methacrylated hydroxymethyl chitosan is respectively 0.4% , 2%, 4%), stirred for 1 h, and then heated in a water bath at 35° C. for 20 min to obtain a uniform hydrogel ink. The methacrylated hydroxymethyl chitosan / hectorite composite hydrogel ink prepared in Example 1 is in a gel state, has certain viscoelasticity and shear thinning, and is suit...

Embodiment 2

[0038]5g chitosan powder is dissolved in the 1wt% acetic acid solution of 200ml, stirs and is heated to 55 ℃, drips methacrylic anhydride (the volume of methacrylic anhydride is 0.1% of acetic acid solution volume) under sealing condition afterwards, After reacting for 3 hours, the solution was dialyzed for 5 days, and then freeze-dried to obtain methacrylated chitosan.

[0039] The product that photoinitiator 2959, hectorite, step (1) obtains is dissolved in pure water successively (the massfraction of photoinitiator 2959, hectorite, methacrylated chitosan is respectively 0.2%, 1.5% , 8%); after stirring for 0.5h, and then heating in a 40°C water bath for 10min, a uniform hydrogel ink was obtained. The methacrylated chitosan / hectorite composite hydrogel ink prepared in Example 2 is in a gel state, has certain viscoelasticity and shear thinning properties, and is suitable for 3D printing. Refer to figure 1 shown.

[0040] Put the hydrogel ink in the printing cylinder, instal...

Embodiment 3

[0042] Dissolve 5g of hydroxypropyl chitosan powder in 250ml of 0.01mol / L phosphate buffer solution, stir and heat to 45°C, then add methacrylic anhydride dropwise under sealed conditions (the volume of methacrylic anhydride is equal to phosphoric acid 1% of the volume of the saline buffer solution), after reacting for 4 hours, the solution was dialyzed for 6 days, and then freeze-dried to obtain methacrylated hydroxypropyl chitosan.

[0043] The product obtained by photoinitiator LAP, hectorite, step (1) is dissolved in pure water successively (the massfraction of photoinitiator LAP, hectorite, methacrylated hydroxypropyl chitosan is respectively 0.3% , 1.5%, 6%), stirred for 1 h, and then heated in a water bath at 25° C. for 30 min to obtain a uniform hydrogel ink. The methacrylated hydroxypropyl chitosan / hectorite composite hydrogel ink prepared in Example 3 is in a gel state, has certain viscoelasticity and shear thinning properties, and is suitable for 3D printing. Refer ...

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Abstract

The invention discloses chitosan / hectorite composite hydrogel for 3D printing as well as a preparation method and application thereof. The method comprises the following steps: under a heating condition, adding methacrylic anhydride into a chitosan (or derivative thereof) solution to react, dialyzing and freeze-drying to obtain methacrylic modified chitosan or derivative thereof; dissolving a photo-initiator, hectorite powder and methacrylic modified chitosan (or derivatives thereof) in water in sequence, uniformly stirring, and heating in a water bath to obtain printable hydrogel ink; carrying out 3D printing on the hydrogel according to a set shape to obtain a three-dimensional bracket, and carrying out ultraviolet curing on the printed bracket for a certain period of time. The shape of the hydrogel bracket can be designed, and the hydrogel bracket has good mechanical properties and biological properties, is free of cytotoxicity and can promote cell growth. Compared with the prior art, the low-content hectorite is used for preparing the chitosan composite hydrogel capable of achieving 3D printing, the preparation method is simple and easy to implement, and application and popularization can be achieved.

Description

technical field [0001] The invention belongs to the field of 3D printing and biomedical materials, and in particular relates to a 3D-printable chitosan / hectorite composite hydrogel and a preparation method and application thereof. Background technique [0002] How to design scaffolds to guide tissue regeneration and repair is a major challenge in tissue engineering. In tissue repair and regeneration, hydrogel scaffolds can provide a microenvironment close to the extracellular matrix for cell proliferation and differentiation, and are an ideal material for tissue repair. [0003] Chitosan and its derivatives have good biocompatibility, biodegradability, antibacterial properties, rich chemical groups (such as amino and hydroxyl) and similar structure to hyaluronic acid, which are suitable as cell scaffolds. However, in most cases, they are not capable of forming mechanically stable gel scaffolds by themselves. Currently, it is an effective method to prepare photocrosslinked ...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L5/08C08K3/34C08B37/08A61L27/44A61L27/50A61L27/52A61L27/58B33Y70/10
Inventor 王小英郑璐黎珊珊
Owner SOUTH CHINA UNIV OF TECH