Fibrous fiber microbacterium, microbial inoculum containing same, and preparation method and application of microbial inoculum
A technology of fiber microbacteria and bacterial agent, which is applied in the field of microbial preparations, and can solve the problems of no Finnish fiber microbacteria and degradation of diuron, etc.
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[0032] The present invention also provides a preparation method for the bacterial agent described in the above scheme, comprising the following steps:
[0033] Inoculate the fibrous microbacteria in LB liquid culture medium, carry out expanded culture, obtain bacterium;
[0034] The initial concentration of the fibrous microbacteria of Finnen is (1~9)×10 9 cfu / mL; the inoculation amount of the microbacteria fenckii is 10% to 20% of the volume of the LB liquid medium. In the present invention, the initial concentration of said fibrous microbacteria is preferably 5.6 × 10 9 cfu / mL; the inoculum size of the microbacteria fenckii is 15% of the volume of the LB liquid medium. In the present invention, the LB liquid culture medium uses water as a solvent, and includes components with the following mass concentrations: 8-12 g / L of tryptone, 3-8 g / L of yeast extract and 8-12 g / L of NaCl.
[0035] In the present invention, the expansion medium preferably includes the following compo...
Embodiment 1
[0056] In 2018, the soil that had been continuously applied with Diuron for more than 10 years was collected in the cotton field of Shizongchang, Beiquan Town, Shihezi City. In the collection medium, the formula of the enriched nutrient solution is: tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, agar powder 15g / L, distilled water 1L. The concentration of diuron is 100mg / L, add 10g of the soil sample to be tested in 90mL of the enrichment culture solution, cultivate it at 30℃ and 180r / min for 7 days, take 10% of the inoculation volume (v:v) liquid, inoculate After continuing to cultivate for 7 days in the enriched culture solution with a diuron concentration of 500 mg / L, the above-mentioned enriched culture solution was inoculated into the enriched culture solution with a content of 1000 mg / L diuron according to an inoculum size of 10%. When transferring to 1500mg / L, 1800mg / L, 2000mg / L continuously in this way, plate coating was carried out, and it was found that when the cont...
Embodiment 2
[0084] By controlling the initial concentration (25, 50, 100, 200, 500mg / L) and inoculation amount (1%, 3%, 5%, 10%, 15% (v:v) of the medicament in the medium, the initial concentration is 5.6×10 9 cfu / mL), temperature (25, 28, 30, 32, 35°C), pH (adjust pH to 5.0, 6.0, 7.0, 8.0, 9.0), additional carbon source (0.0%, 0.5%, 1.0% , 1.5%, 2.0% (m:m) sucrose as an external carbon source) to explore the optimal degradation conditions of Actinomycetes SL-1.
[0085] The results of this test are as Figure 5 shown.
[0086] Depend on Figure 5 As shown, Actinomyces SL-1 has the highest degradation rate of the drug on the fifth day when the drug concentration is 200 mg / L, the inoculum amount is 5%, the pH is 7 at 30°C, and no external carbon source is added. Up to 90.8%.
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