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Atomic force microscope liquid phase imaging method

A technology of atomic force microscope and imaging method, which is applied in the direction of scanning probe microscopy, instruments, measuring devices, etc., can solve problems such as complicated operation, and achieve the effect of simple operation, effective operation and universal use

Active Publication Date: 2021-04-20
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The above method has the following problems: 1. The above method needs to observe the liquid level and the position of the probe through the transparent side wall of the liquid pool in real time; 2. It needs to adjust the laser and drop the liquid many times, and the operation is complicated

Method used

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  • Atomic force microscope liquid phase imaging method

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Experimental program
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Effect test

Embodiment 1

[0051] (1) Use epoxy glue to fix mica slices (circular and 1x1cm in diameter) on the glass slide at the bottom of the liquid pool 2 Square is applicable), let the epoxy resin cure after 12 hours;

[0052] 7U / mL of BspMI with λ-DNA in 10mM CaCl 2 , 25mM KCl, 10mM HEPES buffer, mixed evenly, reacted for 30 minutes, and the reaction temperature was 37°C;

[0053] Drop onto the newly dissociated mica sheet (the mica sheet is fixed on the bottom of the liquid pool or on the slide at the bottom of the BioCell), settle for 2 minutes, and then wash with 10mL of 10mMCaCl 2 , 25mMKCl, 10mM HEPES buffer for gentle washing, and finally wash with 2mL of 10mMNiCl2, 25mMKCl, 10mM HEPES imaging buffer, and add imaging buffer;

[0054] (2) There is no need to adjust the laser. After the sample is placed, use the needle directly in contact mode. The system will automatically stop when the gas-liquid interface is blocked. At this time, use z stepper Motors to manually place the needle 100 μm t...

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Abstract

The invention belongs to the technical field of atomic force microscope application, and particularly relates to an atomic force microscope liquid phase imaging method. The liquid level and the probe position do not need to be observed through the transparent side wall of a liquid pool in real time, that is, after optimization, the method can be widely applied to liquid pools (such as BioCell of a side wall metal shell and the like) and AFM liquid phase platforms of various specifications and materials, and is more universal. According to the method, laser does not need to be adjusted and the liquid does not need to be dropwise added for multiple times, the whole process can be operated on JPK official control software except for one-time necessary adjustment of the laser under the AC mode, and the used contact mode does not need to adjust any parameter, so that the method is simpler and more convenient; the method is easy to operate, effective and universal, and a biological sample with physiological activity can be imaged in a buffer solution for simulating the physiological environment.

Description

technical field [0001] The invention belongs to the field of atomic force microscope application technology, and in particular relates to an atomic force microscope liquid phase imaging method. Background technique [0002] AFM is mainly composed of a laser system (Laser) that provides a light source for the feedback optical path, a micro-cantilever system (Cantilever) for force-distance feedback, and a piezoelectric scanner (x, y, z Piezo-scanner) that performs raster scanning and Z-axis positioning. , photodetector (Detector) receiving optical feedback signal, feedback electronic circuit (Current Circle), rough positioning system, anti-shock and anti-noise system, computer control system and data processing software, sample detection environment control system (humidity control, temperature control, Gas environment control, etc.), monitoring the relative position of the laser-cantilever-sample microscope and CCD camera system, etc. Among them, the first four systems are t...

Claims

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Application Information

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IPC IPC(8): G01Q60/24G01Q60/38
Inventor 王艳伟戴庭辉陈晓伊艺伊浩博吴佳阅吕方怡
Owner WENZHOU UNIVERSITY