Method for determining concentration of imidafenacin in blood plasma by liquid chromatography-mass spectrometry

A midanacin, LC/MS technology, applied in the field of medicine, can solve the problems of speed, precision, sensitivity, selectivity, etc. to be improved, and achieve the effect of high sensitivity, strong specificity, and stable baseline

Inactive Publication Date: 2021-04-30
徐州立顺康达医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the speed, precision, sensitivity, and selectivity of the existing midana assay methods need to be improved

Method used

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  • Method for determining concentration of imidafenacin in blood plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of imidafenacin in blood plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of imidafenacin in blood plasma by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Example: Human K 2 Determination of Midanacin Concentration in EDTA Plasma

[0033] 1. Experimental materials and analytical equipment Midanacin (analyte): TLC Pharmaceutical Standards or the same or higher standard product Midanacin-d10 (internal standard): TLC Pharmaceutical Standards or the same or higher standard The reagents used in the product are shown in Table 1 below:

[0034] Table 1 Reagent Details

[0035] Reagent name level manufacturer Acetonitrile (ACN) HPLC J. T. Baker Ammonium acetate (CH 3 COONH 4 )

HPLC J. T. Baker Formic acid (FA) ACS Adamas Methanol (MeOH) HPLC J. T. Baker

[0036] Note: Reagents of the same grade or higher can also be used

[0037] The analytical equipment used is shown in Table 2 below:

[0038] Table 2 Details of equipment used

[0039]

[0040]

[0041] The same LC / MS / MS system can also be used.

[0042] 2. Liquid conditions

[0043] 1. Liquid chromatograph...

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Abstract

The invention discloses a method for determining the concentration of imidafenacin in blood plasma by liquid chromatography-mass spectrometry. The method adopts a liquid chromatography-mass spectrometry system for determination and comprises the following steps: taking a sample to be detected, adding a certain amount of mixed organic solvent for extraction and pretreatment, separating by using a chromatographic column, and detecting by using a mass spectrum detector. The method is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for measuring the blood concentration of imidafenacin; the linear range of the plasma standard curve of the method is 0.01-2ng / mL, the intra-batch precision RSD and the inter-batch precision RSD are both smaller than + / -15%, and the method is suitable for measuring the concentration of imidafenacin in blood plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a method for determining a drug, in particular to a method for determining the concentration of midanacin in plasma by liquid mass spectrometry. Background technique [0002] Midanacin is suitable for urgency, frequency and incontinence caused by overactive bladder. Bladder contraction is controlled by acetylcholine, mediated by the muscarinic choline receptor (M-type choline receptor) subtype M3. In addition, stimulation of the muscarinic acetylcholine receptor subtype M1 promotes the release of acetylcholine from nerve endings in the bladder. [0003] In vitro, midanacin can antagonize receptor subtypes M3 and M1; in the bladder, midanacin can inhibit the release of acetylcholine through M1 antagonism, and inhibit bladder smooth muscle contraction through M3 antagonism. Compared with the secretion inhibitory effect of salivary glands, midanacin has a stronger in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 范志鸿
Owner 徐州立顺康达医药科技有限公司
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