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Fluorescent nanoprobe and preparation method and application thereof

A fluorescent nanoprobe and probe technology, applied in the field of fluorescent nanoprobe and its preparation, can solve the problems of long elution time, difficult degradation and metabolism, short luminescent time, etc., achieve good biological safety, reduce positive rate, improved prognosis

Pending Publication Date: 2021-05-07
厦门大学附属翔安医院
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Problems solved by technology

[0004] The current tumor-targeting probes are mostly antibody-based fluorescent molecular probes, small-molecule polypeptide probes, and inorganic nanoparticle probes. Among them, antibody-based fluorescent molecular probes have a long elution time, and most of them need to be washed before surgery. 3-4 days of injection, and the preparation price is relatively expensive; small molecule polypeptide probes are not stable enough in the body, and metabolized quickly in the body, resulting in a short luminescence time; inorganic nanoparticles are easy to accumulate in the liver and kidney, and are not easy to degrade and metabolize characteristics, and requires artificial targeting modification

Method used

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  • Fluorescent nanoprobe and preparation method and application thereof
  • Fluorescent nanoprobe and preparation method and application thereof
  • Fluorescent nanoprobe and preparation method and application thereof

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Embodiment

[0041] Such as figure 1 As shown, the present invention discloses a fluorescent nanoprobe, the probe is RGD-HBc / ICG, which includes hepatitis B virus-like protein cage HBc VLP, the surface of the nanocage is fused with RGD peptide by genetic engineering technology, and the interior is loaded with indocyanine Green ICG

[0042] A method for preparing a fluorescent nanoprobe, the method comprising the following steps:

[0043] S1. Based on the amino acid sequence of the HBc-160 protein, insert the RGD sequence between the 78th and 81st amino acids of the HBc-160 protein sequence, and insert two glycine-rich polypeptides GTSGSSGSGSGGSGSGGGG as connecting peptides at both ends of the RGD And obtain RGD-HBc protein;

[0044] S2, transforming the plasmid with the gene of interest into Escherichia coli, the specific steps include:

[0045] S21. Add 1 ng of pET43.1(a)-RGD-HBc plasmid to 100 μL of competent E. coli cell suspension, and place in a water bath at 42°C for 60-90 seconds...

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Abstract

The invention discloses a fluorescent nanoprobe and a preparation method and application thereof. The probe is RGD-HBc / ICG and comprises a hepatitis B virus-like protein cage HBc VLP, RGD peptide is fused on the surface of the probe, and indocyanine green ICG is loaded in the probe. The fluorescent nanoprobe and the preparation method and application thereof are used for distinguishing breast tumors from normal tissue and accurately identifying tumor boundaries, have a remarkable effect on visual tumor surgical incisal edges, reduce the surgical incisal edge positive rate, improve the life quality of patients and prolong the total lifetime, and have important clinical significance for improving prognosis of the patients.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a fluorescent nanoprobe and its preparation method and application. Background technique [0002] Surgery is one of the main treatment methods for solid tumors, and ensuring complete tumor resection is the key to successful surgery. Multiple studies have shown that negative surgical margins can significantly improve the survival time of patients and reduce the need for subsequent adjuvant therapy. For example, in malignant glioma, tumor resection as much as possible can significantly prolong the disease-free survival of patients. In breast cancer In the treatment of breast-conserving surgery, the status of surgical margin is one of the main risk factors affecting the reoperation rate and local recurrence rate of patients. It can be seen that positive surgical margins seriously affect the prognosis of patients and significantly increase medical expenditures. However, after decades of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00C07K14/005C09K11/06C12N15/70
CPCA61K49/0034C07K14/005C09K11/06C12N15/70A61K49/0093A61K49/0056C12N2730/10122C07K2319/00
Inventor 张国君杨瑞钦陈敏任磊
Owner 厦门大学附属翔安医院
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