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Preparation method of copper-containing complex
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A technology of copper complex and glue solution is applied in the field of preparation of copper-containing complex, and can solve the problems of high cost, limited application of cosmetic products, etc.
Pending Publication Date: 2021-05-18
SUZHOU AIBOMAIER NEW MATERIAL CO LTD
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Problems solved by technology
[0003] At present, GHK-Cu is mainly prepared by artificial synthesis, and the cost of the existing method is relatively high, which limits its application in cosmetic products
Method used
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Embodiment 1
[0025] (1) Add 500g of edible gelatin to 10L of 85°C 1% sodium hydroxide aqueous solution, stir for 24 hours, filter the insoluble matter through filter cloth, and cool the filtrate to 60°C;
[0026] (2) Adjust the glue solution to pH = 9.0 with hydrochloric acid, add 30 g of alkaline protease (Bacillus licheniformis), and react at 60°C for 4 hours; heat the glue solution to 90°C and keep it warm for 1 hour, then cool to 40°C;
[0027] (3) Adjust the glue solution to pH=7.8 with hydrochloric acid, add 35g of neutral protease (Bacillus subtilis), and react at 40°C for 4h;
[0028] (4) Adjust the glue solution to pH = 3.0 with hydrochloric acid, add 40% acid protease (Aspergillus niger), and react at 40°C for 4 hours;
[0029] (5) Adjust the glue solution to pH=7.0 with sodium hydroxide, add 100 g of copper hydroxide, stir for 8 hours, and remove insoluble matter with a filter cloth;
[0030] (6) Add 2.5L of absolute ethanol, stir for 1 hour and remove insoluble matter with a f...
Embodiment 2
[0034] (1) Add 500g of edible gelatin to 10L of 85°C 1% sodium hydroxideaqueous solution, stir for 24 hours, filter the insoluble matter through filter cloth, and cool the filtrate to 60°C;
[0035] (2) Adjust the glue solution to pH = 9.0 with hydrochloric acid, add 30 g of alkaline protease (Bacillus licheniformis), and react at 60°C for 4 hours;
[0036] (3) Heating the glue solution to 90°C and keeping it warm for 1h, then cooling to 40°C;
[0037] (4) Adjust the glue solution to pH=7.0 with hydrochloric acid, add 35g of neutral protease (papain), and react at 40°C for 4h;
[0038] (5) Adjust the glue solution to pH = 3.0 with hydrochloric acid, add 40% acid protease (Aspergillus niger), and react at 40°C for 4 hours;
[0039] (6) Adjust the glue solution to pH=7.0 with sodium hydroxide, add 100 g of copper hydroxide, stir for 8 hours, and remove insoluble matter with a filter cloth;
[0040] (7) Add 2.5L of absolute ethanol, stir for 1 hour and remove insoluble matter ...
Embodiment 3
[0044] (1) Add 500g of edible gelatin to 10L of 85°C 1% sodium hydroxide aqueous solution, stir for 24 hours, filter the insoluble matter through filter cloth, and cool the filtrate to 60°C;
[0045] (2) Adjust the glue solution to pH = 9.0 with hydrochloric acid, add 30 g of alkaline protease (Bacillus licheniformis), and microwave at 60 ° C for 15 min;
[0046] (3) Heating the glue solution to 90°C and keeping it warm for 1h, then cooling to 40°C;
[0047] (4) Adjust the glue solution to pH=7.8 with hydrochloric acid, add 35g of neutral protease (Bacillus subtilis), and microwave at 40°C for 15 minutes;
[0048] (5) Adjust the glue solution to pH = 3.0 with hydrochloric acid, add 40% acid protease (Aspergillus niger), and microwave at 40°C for 15 minutes;
[0049] (6) Adjust the glue solution to pH=7.0 with sodium hydroxide, add 200 g of copper hydroxide, stir for 8 hours, and remove insoluble matter with a filter cloth;
[0050] (7) Add 2.5L of absolute ethanol, stir for ...
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Abstract
The invention discloses a copper-containing complex obtained by reacting a collagen hydrolysate with copperhydroxide. A preparation method comprises the following steps of dissolving gelatin with a sodiumhydroxideaqueous solution, and filtering out insoluble substances with filter cloth; adjusting the pH value with hydrochloric acid, and adding alkaline protease for a reaction; heating a gelatin solution for a reaction; adjusting the pH value with hydrochloric acid, and adding neutral protease for a reaction; adjusting the pH value of the gelatin solution with hydrochloric acid, and adding acid protease for a reaction; adjusting the pH value of the gelatin solution with sodiumhydroxide, adding copper hydroxide, performing stirring, and removing insoluble substances with filter cloth; adding absolute ethyl alcohol, performing stirring, and removing insoluble substances by using filter cloth; continuously adding absolute ethyl alcohol, performing stirring, performing filtering with filter cloth, collecting insoluble substances, performing washing with ethyl alcohol, and performing drying in vacuum; and dissolving a dried product in water, and carrying out reduced-pressure spin-drying to obtain the copper-containing complex. The complex has the physiological activity similar to that of GHK-Cu, is lower in manufacturing cost and can be applied to various skin and hair care products.
Description
technical field [0001] The invention belongs to the field of biological materials and relates to a preparation method of a copper-containing complex. Background technique [0002] Copper is an essential trace element for the human body. Copper is a cofactor for many enzymes and a necessary condition for the synthesis of collagen and elastin. It can tighten the skin, resist free radical damage, and even cooperate with vitamin E to repair . It is difficult for free copper ions to enter the skin to take effect through external application. People mainly work together by combining copper with biomass molecules. For example, GHK-Cu is a complex product of copper and tripeptide. Experiments have found that the combination of copper and peptide can stimulate the formation of collagen and elastin, increase the production of mucus between skin cells, assist the antioxidantenzyme SOD, and expand Hair follicles accelerate hair growth and many other functions. [0003] At present, G...
Claims
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Application Information
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