Bacillus subtilis and its fermentation method with high yield of indole acetic acid and high sporulation rate
A technology of Bacillus subtilis and indole acetic acid, which is applied in the field of microbial fermentation, can solve the problems of low acetic acid yield, long spore formation induction time, unstable spore formation rate and the like, and achieves the effect of high spore formation rate
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Embodiment 1
[0037] (1) shake flask culture
[0038] Take the Bacillus subtilis 8-32 preserved on the slant, and use the inoculation loop to inoculate the strain in the shake flask culture medium. The shake flask culture medium is composed of the following components in parts by mass: 2% corn flour, 5% soybean cake powder, Potassium dihydrogen phosphate 0.01%, magnesium sulfate 0.01%, calcium chloride 0.01%, and the balance is water; the shake flask is placed in a shaking incubator, the culture temperature is 30°C, the rotation speed is 150r / min, and culture is carried out for 18 hours to obtain the shaker flask. bottle of seed solution;
[0039] (2) Seed tank fermentation
[0040] The shake flask seed solution was inoculated in the seed medium by volume ratio of 10%, and the seed medium was composed of the following components in parts by mass: yeast extract powder 5%, peptone 2%, sodium chloride 0.03%, magnesium sulfate 0.03% , calcium chloride 0.03%, and the balance is water; control ...
Embodiment 2
[0047] (1) shake flask culture
[0048] Take the Bacillus subtilis 8-32 preserved on the slant, inoculate the strains in the shake flask medium with an inoculation loop, and the shake flask medium consists of the following components in parts by mass: 5% corn flour, 2% soybean cake powder, Potassium dihydrogen phosphate 0.03%, magnesium sulfate 0.03%, calcium chloride 0.03%, and the balance is water; the shake flask is placed in a shaking incubator, the culture temperature is 37°C, the rotation speed is 200r / min, and the shake flask is cultivated for 24h to obtain bottle of seed solution;
[0049] (2) Seed tank fermentation
[0050] Inoculate the shake flask seed liquid at 5% by volume in the seed medium, and the seed medium is composed of the following components in parts by mass: 2% of yeast extract powder, 5% of peptone, 0.01% of sodium chloride, and 0.01% of magnesium sulfate , calcium chloride 0.01%, the balance is water; control seed tank fermentation conditions: the t...
Embodiment 3
[0057] (1) shake flask culture
[0058] Take the Bacillus subtilis 8-32 preserved on the slant, and inoculate the strains in the shake flask medium with an inoculation loop. The shake flask medium consists of the following components in parts by mass: 3.5% glucose, 3.5% soybean cake powder, chlorine 0.02% iron oxide, 0.02% magnesium sulfate, 0.02% calcium chloride, and the balance is water; the shake flask is placed in a shaking incubator, the culture temperature is 35°C, the rotation speed is 180r / min, and culture is carried out for 20 hours to obtain shake flask seeds liquid;
[0059] (2) Seed tank fermentation
[0060] The shake flask seed solution was inoculated in the seed medium by volume ratio of 8%, and the seed medium was composed of the following components in parts by mass: 3.5% of yeast extract powder, 3.5% of peptone, 0.02% of sodium chloride, and 0.02% of magnesium sulfate , calcium chloride 0.02%, and the balance is water; control the seed tank fermentation co...
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