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Method for producing metabiotics by carrying out damp-heat quenching on semi-immobilized probiotics

A technology of probiotics and moist heat, applied in the direction of Lactobacillus, bacteria used in food preparation, food science, etc., to achieve the effects of improving immunity, improving intestinal immunity, and fast moisture-based heat conduction

Active Publication Date: 2021-05-28
山东泰山生力源集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, epigenetic elements are emerging concepts in recent years, and there are few relevant reports on the preparation methods of epigenetic elements.

Method used

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  • Method for producing metabiotics by carrying out damp-heat quenching on semi-immobilized probiotics
  • Method for producing metabiotics by carrying out damp-heat quenching on semi-immobilized probiotics

Examples

Experimental program
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Embodiment 1

[0029] (1) Preparation of lactic acid bacteria fermentation broth: after activating and purifying the lactic acid bacteria strains, culture them in MRS medium to obtain lactic acid bacteria fermentation broth.

[0030] (2) Centrifugal concentration of fermented liquid: the lactic acid bacteria fermented liquid is centrifuged and concentrated in a disc centrifuge until the concentration of the bacterial liquid is 1×10 11 cfu / g to obtain the lactic acid bacteria concentrate.

[0031] (3) Adsorption and immobilization of lactic acid bacteria: the concentrated solution of lactic acid bacteria and soybean fiber powder were mixed according to the ratio of 2:1 to obtain a mixture.

[0032] (4) Steam damp heat quenching activity: The mixture was inactivated at 100° C. for 5 minutes by steam damp heat sterilization.

[0033] (5) Rapid cooling: the inactivated mixture is rapidly cooled by liquid nitrogen and cold air simultaneously, so that the temperature of the mixture rapidly drops ...

Embodiment 2

[0036] (1) Preparation of lactic acid bacteria fermentation broth: after activating and purifying the lactic acid bacteria strains, culture them in MRS medium to obtain lactic acid bacteria fermentation broth.

[0037] (2) Centrifugal concentration of fermented liquid: the lactic acid bacteria fermented liquid is centrifuged and concentrated in a disc centrifuge until the concentration of the bacterial liquid is 1×10 11 cfu / g to obtain the lactic acid bacteria concentrate.

[0038] (3) Adsorption and immobilization of lactic acid bacteria: the concentrated solution of lactic acid bacteria and soybean fiber powder are mixed according to the ratio of 2.5:1 to obtain a mixture.

[0039] (4) Steam damp heat quenching activity: the mixture was inactivated at 100° C. for 10 min by steam damp heat sterilization.

[0040] (5) Rapid cooling: the inactivated mixture is rapidly cooled by means of dry ice and cold wind at the same time, so that the temperature of the mixture rapidly drop...

Embodiment 3

[0043] (1) Preparation of lactic acid bacteria fermentation broth: after activating and purifying the lactic acid bacteria strains, culture them in MRS medium to obtain lactic acid bacteria fermentation broth.

[0044] (2) Centrifugal concentration of fermented liquid: the lactic acid bacteria fermented liquid is centrifuged and concentrated in a disc centrifuge until the concentration of the bacterial liquid is 1×10 11 cfu / g to obtain the lactic acid bacteria concentrate.

[0045] (3) Adsorption and immobilization of lactic acid bacteria: the concentrated solution of lactic acid bacteria and soybean fiber powder are mixed according to the ratio of 2:1.2 to obtain a mixture.

[0046] (4) Steam damp heat quenching activity: The mixture was inactivated at 100° C. for 7.5 minutes by steam damp heat sterilization.

[0047] (5) Rapid cooling: the inactivated mixture is rapidly cooled by means of ice water and cold air simultaneously, so that the temperature of the mixture rapidly ...

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Abstract

The invention discloses a method for producing metabiotics by damp-heat quenching of semi-immobilized probiotics, and the method comprises the following steps: culturing lactic acid bacteria strains through an MRS culture medium to obtain lactic acid bacteria fermentation liquor; concentrating the lactic acid bacteria fermentation liquid until the concentration of the bacterial liquid is 1 * 10 < 11 > cfu / g; mixing the lactic acid bacteria concentrated solution with the soybean fiber powder to obtain a mixture; carrying out inactivation treatment on the mixture by adopting a steam moist heat sterilization method to obtain an inactivated mixture; rapidly cooling the inactivated mixture, so that the temperature of the mixture is reduced to normal temperature within 1-3 minutes; and drying the cooled and inactivated mixture until the water content is 8-10%. According to the invention, the metagen product with a probiotic function and a long shelf life can be produced. Meanwhile, through the damp-heat quenching and rapid cooling technology, thallus splitting decomposition and denaturation can be reduced, and the integrity of thalli is kept. In addition, the wet base is high in thermal conductivity, low in later drying cost, loose and convenient to process.

Description

technical field [0001] The invention belongs to the field of microbial preparations, and relates to a method for producing postbiotics by the damp heat quenching technology of semi-immobilized probiotics. Background technique [0002] With the development of the economy and the continuous improvement of people's living standards, the development of functional foods has attracted more and more attention. Probiotics, also known as live bacterial preparations, can promote the ecological balance of intestinal flora, repel and compete for pathogenic bacteria to colonize in the epithelial cells of the digestive tract, compete with pathogenic bacteria for nutrients, produce antibacterial products, improve the intestinal environment and maintain the mucosal barrier function , activate the host immune response, enhance the immune function of the body. However, in the actual application process, due to the poor resistance of probiotics to the external environment, a large number of d...

Claims

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Application Information

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IPC IPC(8): A23K10/30A23K10/16
CPCA23K10/30A23K10/16A23V2400/169Y02P60/87
Inventor 任小杰高洁李军训马蓉赵学峰
Owner 山东泰山生力源集团股份有限公司
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