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Method for separating neotriptoside from hesperidin waste liquid

A technology of acitrin and hesperidin, which is applied in the field of separation of neocitrin, can solve the problems of no new extraction method, etc., and achieve the effects of easy industrial production, solving sewage treatment problems, and simple operation

Active Publication Date: 2021-05-28
LIANYUAN KANGLU BIOLOGICAL TECH CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides a method for isolating neocitrudinus from hesperidin waste liquid, so as to solve the technical problem that there is no effective extraction method for neocitonin in the prior art

Method used

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  • Method for separating neotriptoside from hesperidin waste liquid
  • Method for separating neotriptoside from hesperidin waste liquid
  • Method for separating neotriptoside from hesperidin waste liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The method for isolating neocitrudin from hesperidin waste liquid comprises the following steps:

[0059] S1. Prepare the mother liquor of hesperidin, pass the mother liquor of hesperidin through a strong basic cationic resin, remove synephrine, and collect the effluent;

[0060] S2, the 10t effluent in step S1 is passed through 500kg HPD100 macroporous adsorption resin, followed by water elution, 50% ethanol elution, and 70% ethanol elution, and the flow rate of the effluent through HPD100 macroporous adsorption resin is 5BV / h , the volume of water elution is 2BV, the flow rate of water elution is 1BV / h, the volume of 50% ethanol elution is 3BV, the flow rate of 50% ethanol elution is 1BV / h; the volume of 70% ethanol elution is 3BV , the flow rate of 70% ethanol elution is 1BV / h, the 70% ethanol eluate is collected, passed through a 100nm ceramic membrane, and then concentrated through a 1000Da nanofiltration membrane, concentrated to 1 / 200 of the volume of the hesperi...

Embodiment 2

[0065] The method for isolating neocitrudin from hesperidin waste liquid comprises the following steps:

[0066] S1. Prepare the mother liquor of hesperidin, pass the mother liquor of hesperidin through a strong basic cationic resin, remove synephrine, and collect the effluent;

[0067] S2, the 20t effluent in step S1 is passed through 500kg D101 macroporous adsorption resin, followed by water elution, 30% ethanol elution, and 80% ethanol elution, and the flow rate of the effluent through D101 macroporous adsorption resin is 4BV / h , the volume of water elution is 4BV, the flow rate of water elution is 2BV / h, the volume of 30% ethanol elution is 3BV, the flow rate of 30% ethanol elution is 2BV / h; the volume of 80% ethanol elution is 3BV , the flow rate of 80% ethanol elution is 1BV / h, the 80% ethanol eluate is collected, passed through a 50nm ceramic membrane, and then concentrated through a 500Da nanofiltration membrane, concentrated to 1 / 100 of the volume of the hesperidin mo...

Embodiment 3

[0072]The method for isolating neocitrudin from hesperidin waste liquid comprises the following steps:

[0073] S1. Prepare the mother liquor of hesperidin, pass the mother liquor of hesperidin through a strong basic cationic resin, remove synephrine, and collect the effluent;

[0074] S2, the 20t effluent in step S1 is passed through 500kg AB-8 macroporous adsorption resin, and water elution, 40% ethanol elution, 70% ethanol elution are carried out successively, and the flow rate of effluent through AB-8 macroporous adsorption resin 5BV / h, the volume of water elution is 4BV, the flow rate of water elution is 2BV / h, the volume of 40% ethanol elution is 3BV, the flow rate of 40% ethanol elution is 1BV / h; 70% ethanol elution The volume is 4BV, and the flow rate of 70% ethanol elution is 1BV / h. The 70% ethanol eluate is collected, passed through a 50nm ceramic membrane, and then concentrated through an 800Da nanofiltration membrane to 1 / 1 of the volume of the hesperidin mother li...

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Abstract

The invention discloses a method for separating neotriptoside from hesperidin waste liquid. The method comprises the following steps: S1, preparing hesperidin mother liquid, passing the hesperidin mother liquid through strongly basic cationic resin, removing synephrine, and collecting effluent; s2, enabling the effluent in the step S1 to pass through macroporous adsorption resin, sequentially carrying out water elution, 10%-50% ethanol elution and 60%-90% ethanol elution, collecting 60%-90% ethanol eluent, carrying out ultrafiltration, and concentrating through a nanofiltration membrane to obtain trapped fluid; s3, cooling and crystallizing the trapped fluid in the step S2 to obtain a new hovenia glycoside coarse crystal; and s4, adding the new hovenia glycoside coarse crystal in the step S3 into the organic mixed solution, mixing, adding activated carbon, heating, refluxing, filtering, concentrating, and cooling and crystallizing the concentrated solution to obtain the new hovenia glycoside. According to the method for separating the neotriptoside from the hesperidin waste liquid, the hesperidin waste liquid is reused, the neotriptoside is extracted from the hesperidin waste liquid, economic benefits are obtained, and the problem of sewage treatment is also solved.

Description

technical field [0001] The invention relates to the field of separation of neocitrus glycosides, in particular to a method for separating neocitrus glycosides from hesperidin waste liquid. Background technique [0002] Liver cancer is one of the most common malignant tumors with a high mortality rate both in my country and in the world. In recent years, the search for anticancer drugs in natural medicines has gradually attracted widespread attention. Epidemiological studies have shown that eating more flavonoid-rich fruits and vegetables can reduce the incidence of cancer. Neocitrin is a flavonoid compound mainly found in citrus fruits and herbal medicines such as oregano. Studies have shown that Neocitrin can induce apoptosis in A549 and NCI-H460 cells through the Fas / Fas ligand apoptosis system. death, and it was confirmed by nude mouse experiments that neocitritoside can be used as a new tumor chemotherapy agent to delay the growth of non-small cell lung cancer. Other ...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/06
CPCC07H17/07C07H1/06
Inventor 肖业成魏有何刘冬敏廖深刻吴爱燕
Owner LIANYUAN KANGLU BIOLOGICAL TECH CO LTD
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