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Method for extracting and purifying active ingredients from cistanche tubulosa

A technology of Cistanche tubulosa and active ingredients, which can be applied to medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulas, etc., and can solve the problem of poor processing technology and degradation loss of active ingredients phenylethanol glycosides , degradation loss and other issues, to achieve the effect of less heat production, short extraction time, and inhibition of enzyme activity

Inactive Publication Date: 2021-06-01
HETIAN DCM MEDICINE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, poor processing technology and drying treatment method will cause enzyme-catalyzed reaction and cause degradation loss of phenylethanol glycosides, which are rich in active ingredients of Cistanche deserticola, especially two representative active ingredients echinacoside and verbascoside monomer compounds. important factor

Method used

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  • Method for extracting and purifying active ingredients from cistanche tubulosa
  • Method for extracting and purifying active ingredients from cistanche tubulosa

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A method for extracting and purifying active ingredients from Cistanche tubulosa, comprising the following steps:

[0031] (1) Take fresh Cistanche tubulosa, wash it, grind it at room temperature and pulverize it into a 200-mesh powder, add water 1.5 times the weight of Cistanche tubulosa, then add an appropriate amount of ascorbic acid, stir and mix evenly;

[0032] (2) Add 75% ethanol with a volume ratio of 2:1 to the product obtained in step (1), then place it at room temperature, with an electric field strength of 85kV / cm, and under the condition of 60 pulses, extract for 10min, press filter , collect the extract;

[0033] (3) Add dodecyl dimethyl betaine to the extract, stir for 20 min, then add diallyl ether and copper chloride, and react for 20 min; wherein, the dodecyl dimethyl betaine The addition amount is 1.5% of the weight of Cistanche tubulosa; the addition of diallyl ether is 0.5% of the weight of Cistanche tubulosa;

[0034] (4) adjust the pH value of t...

Embodiment 2

[0037] A method for extracting and purifying active ingredients from Cistanche tubulosa, comprising the following steps:

[0038] (1) Take fresh Cistanche tubulosa, wash it, grind it at room temperature and pulverize it into a 200-mesh powder, add water 1 times the weight of Cistanche tubulosa, then add ascorbic acid, stir and mix evenly;

[0039] (2) Add 70% ethanol with a volume ratio of 1:1 to the product obtained in step (1), then place it at room temperature, with an electric field strength of 90kV / cm, and under the condition of 50 pulses, extract for 10min, press filter , collect the extract;

[0040] (3) Add dodecyl dimethyl betaine to the extract, stir for 30 minutes, then add diallyl ether and copper chloride, and react for 15 minutes; wherein, the addition of dodecyl dimethyl betaine The amount is 2% of the weight of Cistanche tubulosa; the addition of diallyl ether is 0.8% of the weight of Cistanche tubulosa;

[0041](4) adjust the pH value of the product obtained...

Embodiment 3

[0044] A method for extracting and purifying active ingredients from Cistanche tubulosa, comprising the following steps:

[0045] (1) Take fresh Cistanche tuberosa, wash it, grind it at room temperature and pulverize it into a 200-mesh powder, add water 1.5 times the weight of Cistanche tuberose, then add ascorbic acid, stir and mix evenly;

[0046] (2) Add 80% ethanol with a volume ratio of 2:1 to the product obtained in step (1), then place it at room temperature, with an electric field strength of 60kV / cm, and under the condition of 70 pulses, extract for 30min and press filter , collect the extract;

[0047] (3) Add dodecyl dimethyl betaine to the extract, stir for 30 minutes, then add diallyl ether and copper chloride, and react for 30 minutes; wherein, the addition of dodecyl dimethyl betaine The amount is 0.5% of the weight of Cistanche tubulosa; the addition of diallyl ether is 0.1% of the weight of Cistanche tubulosa;

[0048] (4) adjust the pH value of the product ...

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Abstract

The invention discloses a method for extracting and purifying active ingredients from cistanche tubulosa. The method comprises the following steps: (1) grinding fresh cistanche tubulosa into powder, adding water, adding ascorbic acid, and uniformly stirring and mixing; (2) adding ethanol into the product obtained in the step (1), then placing at room temperature, extracting, carrying out filter pressing, and collecting an extracting solution; (3) adding an ionic surfactant into the extracting solution, stirring, then adding diallyl ether and a catalyst, and reacting; (4) adjusting the pH value of the product obtained in the step (2) to 1-3, stirring, standing, and centrifuging; adjusting the pH value to 7-8, adding ozone to react, and concentrating the extracting solution under reduced pressure; and (5) adding the extracting solution into a macroporous resin column, carrying out gradient elution, collecting and combining eluent, and concentrating and crystallizing. The invention can effectively reduce the loss of effective components in the extraction process of cistanche tubulosa.

Description

technical field [0001] The invention belongs to the technical field of compound extraction and preparation, and in particular relates to a method for extracting and purifying active ingredients from Cistanche tubulosa. Background technique [0002] Herba Cistanches (Herba Cistanches) is a well-known traditional tonic Chinese medicine. The Cistanche deserticola recorded in the 2005 edition of "Chinese Pharmacopoeia" is the dry fleshy stem with scaly leaves of Cistanche deserticola Y.C.Ma and Cistanche deserticola (Schrenk) R.Wight. "Shen Nong's Materia Medica" states: Cistanche "maintains the five labors and seven injuries. It is because the fire is weak and cannot produce soil, and it is not the root of the Qi. In the treatment of women's diseases, salty can soften the hardness and remove the blood. Cistanche stops the discharge of essence Drowning, remove the heat carbuncle in the stem, because it can guide the fire of asthenia, and the elderly are dry and stagnant, so it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/64A61K125/00
CPCA61K36/64A61K2236/15A61K2236/333A61K2236/53A61K2236/55
Inventor 游林贺金华毛艳俞新周佳佳
Owner HETIAN DCM MEDICINE BIOLOGICAL TECH
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