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Method for increasing yield of odd chain fatty acid in grease produced by fermentation of schizochytrium

A technology of Schizochytrium and fatty acids, which is applied in the field of increasing the output of odd-numbered carbon fatty acids in oil produced by fermentation of Schizochytrium, and can solve the problems of high cost of genetic modification, low oil yield, and low biomass

Active Publication Date: 2021-06-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] There have been many reports on lipid production by Schizochytrium fermentation, but microbial fermentation to produce OCFA requires propionate induction, low biomass, low oil production, and low OCFA content
The cost of genetic modification is high, and most of them are in the stage of theoretical research. The highest value of OCFA obtained through genetic engineering reported in the existing literature is 3.32g / L

Method used

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  • Method for increasing yield of odd chain fatty acid in grease produced by fermentation of schizochytrium
  • Method for increasing yield of odd chain fatty acid in grease produced by fermentation of schizochytrium
  • Method for increasing yield of odd chain fatty acid in grease produced by fermentation of schizochytrium

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Effect test

Embodiment 1

[0033] The effect of the total carbon to nitrogen ratio on the content of oil and fatty acids in Schizochytrium was investigated. 500mL baffle shaker flask, liquid volume 100mL, rotation speed 180rpm, glucose was used as the carbon source and sodium glutamate was the main nitrogen source in the Schizochytrium culture medium respectively, and the total carbon and nitrogen mass ratio was set to 20:1 respectively, 10:1, 5:1, when the fermentation reached 120h, the bacterial cells were collected for component analysis. The parameters and results of the fermentation process are shown in Table 1. The bacteria grew rapidly in the first 48 hours of fermentation, and the density and dry weight of the bacteria increased rapidly. After 2 days of fermentation, the dry weight and cell density of all groups reached the maximum. Among them, when the mass ratio of total carbon to nitrogen was 10:1, the cell density and dry cell weight were significantly better than those of the excess nitroge...

Embodiment 2

[0036] The effects of different organic nitrogen sources and ratios of organic nitrogen sources on the production of OCFA by Schizochytrium were investigated. Set up 6 groups of experiments, the mass ratio of organic nitrogen N1 and inorganic nitrogen N2 is set to 1:1; 100mL, fermented for 5 days, and each group was repeated twice. Based on the conclusion of Example 1, the total carbon-nitrogen mass ratio was set to 10:1, and other conditions were the same as in Example 1. The results are shown in Table 2. The organic nitrogen source was beneficial to the growth of the bacteria, and the growth rate of the bacteria increased with the increase of the proportion of the organic nitrogen source. Inorganic nitrogen sources are beneficial to the accumulation of odd-numbered carbon fatty acids, and the proportion of odd-numbered carbon fatty acids in the total fatty acids reached the highest value of 15.75% in the all-inorganic nitrogen source group on the third day of fermentation, a...

Embodiment 3

[0039] To investigate the effect of temperature on the production of OCFA by Schizochytrium, three temperatures were set in this experiment, namely 23°C, 28°C and 33°C, the liquid volume in a 5L fermentation tank was 3L, the pH was adjusted to 7 by hydrochloric acid and sodium hydroxide, and the rotation speed was 600rpm , the ventilation is 3vvm, and the other conditions are consistent with the shake flask. After 120 hours of fermentation, samples were taken every 12 hours for evaluation of fermentation parameters. The results are shown in Table 3. It can be seen from Table 3 that in the 23°C fermentation group, the bacteria grew faster. At the end of the fermentation, the oil production and oil content in dry weight percentage reached the maximum, which were 18.78g / L and 24.31%, respectively. After 3 days of fermentation, the cell density and dry cell weight reached the maximum values, which were 127.8 and 78.25 g / L, respectively. At the same time, the proportion of odd-num...

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Abstract

The invention relates to a method for increasing the yield of odd chain fatty acid in grease produced by fermentation of schizochytrium. The method comprises the following steps: inoculating schizochytrium sp. 27173-E into a fermentation culture medium, carrying out low-temperature aerobic fermentation, and controlling the mass ratio of a total carbon source to a total nitrogen source in the culture medium to be 5:1-20:1, the temperature to be 15-35 DEG C and the fermentation time to be 60-120 hours. The optimal conditions for producing the OCFA by fermenting the schizochytrium 27173-E are as follows: the mass ratio of a total carbon source to a nitrogen source is 10:1, the ratio of organic nitrogen to inorganic nitrogen is 8:1, the fermentation temperature is 23 DEG C, and the fermentation time is 84 hours. By applying the method, the maximum dry weight of cells in the obtained fermentation liquor reaches 78.87 g / L, the OCFA accounts for 31.82% of the total fatty acid, and the yield of the OCFA is 7.48 g / L. According to the invention, the yield of OCFA is greatly improved, and the application potential of schizochytrium is further expanded.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for increasing the output of odd-numbered carbon fatty acids in oil produced by fermentation of Schizochytrium fungus. Background technique [0002] Odd chain fatty acid (OCFA) functions similarly to unsaturated fatty acids and helps to improve the fluidity of cell membranes. It is more abundant in some bacteria, but not more than 1% in animals and lower plants. Odd-carbon fatty acids are metabolized to produce sugar residues, which have better nutritional effects; in addition, triglycerides containing odd-carbon fatty acids can reduce the frequency of secondary complications of sepsis and intensive care treatment, or reduce inflammation sex or shorten the course of disease (CN200980145702.6). In recent years, studies have found that the odd-numbered carbon chain fatty acids in the human body, which are less in content, have important physiological fun...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/14C12R1/645
CPCC12P7/6463C12P7/6409C12N1/14Y02E50/10
Inventor 蓝东明雷长梅肖正王永华莫舒欣
Owner SOUTH CHINA UNIV OF TECH
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