Method for preparing silage through combination of mold for producing cellulase and lactic acid bacteria

A cellulase and combined preparation technology, applied in the preservation method of animal feed raw materials, animal feed, application, etc., can solve the problems of moldy and deteriorated silage, which cannot be fully realized, and affects the quality and economic benefits of silage products.

Inactive Publication Date: 2021-06-04
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the existing silage production, in order to ensure the quality of the silage, the contact between the feed and oxygen should be reduced as much as possible during the silage process, bu

Method used

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  • Method for preparing silage through combination of mold for producing cellulase and lactic acid bacteria
  • Method for preparing silage through combination of mold for producing cellulase and lactic acid bacteria

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specific Embodiment approach 1

[0015] Specific embodiment one: the method that the mold that produces cellulase in this embodiment and lactic acid bacteria jointly prepares silage, it is characterized in that said method realizes according to the following steps:

[0016] 1. Preparation of lactic acid bacteria liquid and mold spore suspension producing cellulase;

[0017] 2. Preparation of lactic acid bacteria liquid starch liquid: add starch to the lactic acid bacteria liquid to make starch-containing lactic acid bacteria liquid;

[0018] 3. Crushing and filling of straw: After crushing the straw, add lactic acid bacteria starch solution and mold spore suspension that produces cellulase while stirring, and pack and seal after stirring evenly;

[0019] 4. Silage fermentation: the silage bag is naturally fermented in a cool, rain-proof, room temperature environment to a pH below 4.2, that is, the silage feed is obtained;

[0020] Wherein, the mold producing cellulase is Aspergillus usamil 3.2926; the straw ...

specific Embodiment approach 2

[0021] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the concentration of starch in the starch-containing lactic acid bacteria liquid in Step 2 is 2.0% to 5.0%. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0022] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that: in step one, lactic acid bacteria undergo secondary activation and proliferation in the MRS medium, and the effective number of viable lactic acid bacteria in the bacterial liquid reaches 1×10 8 ~1×10 9 CFU / ml. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

[0023] The lactic acid bacteria in this embodiment are lactobacillus plantarum (lactobacillus plantarum) 1.11; the culture conditions of plant lactobacillus (lactobacillus plantarum) 1.11 are constant temperature 30 degrees, static culture, culture 18hr to 24hr, the number of effective viable bacteria can reach 1×10 8 ~10 9 CFU / ml.

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Abstract

The invention relates to a method for preparing silage through combination of mold for producing cellulase and lactic acid bacteria. The method for preparing the silage through combination of mold for producing cellulase and lactic acid bacteria comprises the following steps of 1, preparing lactic acid bacteria liquid and mold spore suspension for producing cellulase; 2, preparing lactic acid bacteria starch liquid; 3, smashing of straws and filling: smashing the straws, adding the lactic acid bacteria liquid containing starch and mold spore suspension for producing cellulase while stirring, and packaging and sealing after uniformly stirring; and 4, silage fermentation: naturally fermenting the silage bags in a shady, cool and rain-sheltering room-temperature environment until the pH value is less than 4.2 to obtain the silage. Growth and reproduction of aerobic putrefying bacteria in the silage are reduced, putrefaction and deterioration of the silage are avoided or relieved, the effective recovery rate of the silage is increased, and meanwhile the content of neutral and acid detergent fibers is reduced by degrading cellulose macromolecules, so that the quality of the silage is improved.

Description

technical field [0001] The invention relates to a feed and a preparation method thereof. Background technique [0002] With the development of the economy and the progress of the society, people's requirements for the quality of life are gradually increasing, and the consumption of dairy products is increasing year by year. However, the number and milk production of dairy cows (sheep) cannot meet the needs of consumption growth. A major constraint is the availability of high-quality "feed". In order to conserve vegetation, many areas of my country have banned grazing, and at the same time, in order to reduce the dependence of animal husbandry on grain, corn stalks, which are rich in resources, have become the most ideal source. However, corn stalks are relatively seasonal. In order to allow livestock to have sufficient and nutritious feed all year round, silage is often used for processing. [0003] Silage refers to the feed made of green feed through controlled fermentati...

Claims

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Application Information

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IPC IPC(8): A23K30/18
CPCA23K30/18
Inventor 原韬曹亚彬牛彦波安琦殷博董博吴皓琼
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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