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Flexible sulfide-producing bacterium quantity measuring sensor and method for measuring SRB quantity in sewage by indirect method

A sulfide generation and sensor technology, which is applied to electrochemical variables of materials, instruments, measurement devices, etc., can solve the problems of simple structure calibration and large measurement numerical errors, and achieve the effect of simple measurement methods.

Active Publication Date: 2021-06-04
CHINA PETROLEUM & CHEM CORP +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This utility model can be used to measure the content of sulfate-reducing bacteria in situ, which is convenient and fast, and the test speed is fast. However, due to its simple structure, it cannot be calibrated well, resulting in large errors in measurement values.

Method used

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  • Flexible sulfide-producing bacterium quantity measuring sensor and method for measuring SRB quantity in sewage by indirect method
  • Flexible sulfide-producing bacterium quantity measuring sensor and method for measuring SRB quantity in sewage by indirect method
  • Flexible sulfide-producing bacterium quantity measuring sensor and method for measuring SRB quantity in sewage by indirect method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Preparation of working electrode:

[0059] Such as Figure 1-Figure 4 As shown in, in a preferred embodiment, a flexible sulfide-producing bacteria count sensor of the present invention includes a working electrode, a reference electrode and an auxiliary electrode; wherein, the carbon cloth containing the active material is used as the working The electrode uses a saturated calomel electrode as a reference electrode, and a metal platinum electrode as an auxiliary electrode; wherein, the working electrode uses carbon cloth as a base material, and a ZnO nanoneedle array is electrodeposited on the surface of the carbon cloth to form a carbon cloth / ZnO composite material, and then the carbon cloth / ZnO composite material is hydrothermally reacted to obtain a carbon cloth / ZnO@ZIF-8 nanocolumn array, which is a working electrode.

[0060] Among them, carbon cloth / ZnO means that ZnO is generated on the surface of carbon cloth; in a hydrothermal kettle, ZnO on the surface of c...

Embodiment 2

[0066] Obtaining the linear relationship between the log value of the sulfide ion concentration and Rct:

[0067] Take sodium sulfide and prepare five sodium sulfide standard solutions with sulfide ion content of 0.5 μmol, 5 μmol, 50 μmol, 0.5 mmol, and 5 mmol respectively, and then take a portion of deionized water as a blank sample. First measure the resistance value of the aforementioned six liquids, and get Figure 5 , that is, the relationship curve between the sulfur ion content and the electrochemical resistance of the solution. It can be seen from the curve that the logarithmic value of the sulfur ion concentration is linearly related to the electrochemical resistance of the solution.

[0068] With the sensor in embodiment 1, get six working electrodes, soak 30min in six parts of liquids respectively, make the sulfide ion in the solution fully react with the ZIF-8 on the working electrode surface, then measure the electrochemistry that this solution sample presents. I...

Embodiment 3

[0072]Obtaining the standard curve of the linear relationship between the amount of SRB bacteria and electrochemical impedance:

[0073] Get the spot sewage, add enough zinc acetate solids in the spot sewage, make the sulfide ion impurity in the described spot sewage thoroughly remove, after various impurities are precipitated through centrifugation, get 10ml supernatant A, the described Supernatant A was autoclaved at 121°C for 20 minutes to remove other bacteria in the supernatant A; after mixing 10ml of the supernatant A with 90ml of SRB medium, place it in a constant temperature incubator at 37°C Incubation, sampling respectively according to different incubation times to obtain several standard samples with different SRB bacterial content, and using the MPN method to detect the SRB bacterial content of each standard sample; The electrochemical impedance value of above-mentioned standard sample solution; Adopt the measurement condition identical with embodiment 2 again (th...

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Abstract

The invention relates to a flexible sulfide-producing bacterium quantity measuring sensor and a method for measuring the SRB quantity in sewage by an indirect method. The flexible sulfide-producing bacterium quantity measuring sensor comprises a working electrode, a reference electrode and an auxiliary electrode. Carbon cloth containing an active material is used as a working electrode, a saturated calomel electrode is used as a reference electrode, and a metal platinum electrode is used as an auxiliary electrode. The indirect determination method is obtained through calculation according to a sulfide concentration and electrochemical impedance relation standard curve and a bacteria quantity and electrochemical impedance relation standard curve. The method disclosed by the invention has the advantages of short time for measuring the bacterial quantity in the field sewage, high accuracy and universal application significance.

Description

technical field [0001] The invention belongs to the field of petroleum engineering, in particular to the technical field of industrial water treatment and environmental protection on the oilfield site, and particularly relates to a sensor and a method for measuring the amount of flexible sulfide-producing bacteria. Background technique [0002] Many studies have shown that microbial corrosion is the main form of pipeline failure in water systems, and the corrosion characteristics are general uneven pitting corrosion. In a relatively closed water environment system, corrosive microorganisms colonize the surface of the substrate material to form a layer of microbial film, which is mainly composed of microbial cell bodies, corrosion products and scale layers formed by inorganic ions in the environmental water medium. In organic biofilms, the extracellular polymers secreted by microorganisms such as bacteria act as a binder, and at the same time endow the bacteria in the membran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/333G01N27/32G01N27/327
CPCG01N27/308G01N27/333G01N27/301G01N27/32G01N27/327G01N27/30
Inventor 刘晶姝周海刚刘宏芳龙媛媛董晓通王亭沂谭晓林杨为刚王军磊李胜
Owner CHINA PETROLEUM & CHEM CORP
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