Cultivation method of black-skin termitomyces albuminosus
A cultivation method and technology of chicken fir, applied in cultivation, plant cultivation, mushroom cultivation, etc.
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Embodiment 1
[0037] S1. Cultivation of mother species: Pick a small piece of tissue from the fruiting body of the black-skinned fir tree that has been sterilized on the surface, and transfer it to the culture medium of the mother species. The temperature is controlled at 20-25°C. Kind; The mother seed culture medium consists of: glucose 40g, peptone 3g, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, vitamin B 1 10mg, 20g agar, 1000g water, PH7.0;
[0038] S2. Cultivation of original seeds and cultivars: insert the mother species cultivated in S1 into the original seed medium, cultivate and prepare the original seeds, and then transplant the original seeds to the cultivar medium for further expansion and cultivation to obtain the required cultivation species; the original seed medium and cultivated medium are composed of: cottonseed hull 50g, sawdust 29g, wheat bran 20g, gypsum 1g, water content 60g;
[0039] S3, artificial cultivation process, specifically comprises the followin...
Embodiment 2
[0052] S1. Cultivation of mother species: Pick a small piece of tissue from the fruiting body of the black-skinned fir tree that has been sterilized on the surface, and transfer it to the culture medium of the mother species. The temperature is controlled at 20-25°C. species; the mother seed medium consists of: potato 20g, glucose 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar 20g, water 1000ml, pH 7.0;
[0053] S2. Cultivation of original seeds and cultivars: insert the mother species cultivated in S1 into the original seed medium, cultivate and prepare the original seeds, and then transplant the original seeds to the cultivar medium for further expansion and cultivation to obtain the required cultivation The original seed culture medium and the cultivar culture medium consist of: cottonseed hull 35g, rice straw 25g, sawdust 19g, wheat bran 20g, gypsum 1g, water content 60g;
[0054] S3, artificial cultivation process, specifically comprises the followin...
Embodiment 3
[0067] S1. Cultivation of mother species: Pick a small piece of tissue from the fruiting body of the black-skinned fir tree that has been sterilized on the surface, and transfer it to the culture medium of the mother species. The temperature is controlled at 20-25°C. species; the mother seed culture medium consists of: glucose 35g, peptone 4g, potassium dihydrogen phosphate 3g, magnesium sulfate 2g, vitamin B 1 5mg, 20g agar, 1200g water, PH7.0;
[0068] S2. Cultivation of original seeds and cultivars: insert the mother species cultivated in S1 into the original seed medium, cultivate and prepare the original seeds, and then transplant the original seeds to the cultivar medium for further expansion and cultivation to obtain the required cultivation species; the original seed medium and cultivar medium consist of: 40g of cottonseed hulls, 27g of miscellaneous sawdust, 30g of wheat bran, 3g of gypsum, and 70g of water content.
[0069] S3, artificial cultivation process, specif...
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