Salbutamol impurity compound as well as preparation method, detection method and application thereof
A technology of salbutamol and its detection method, which is applied in the field of medicine, can solve the problems of salbutamol's toxic and side effects, the production of process impurities and degradation impurities, and the impact on drug quality, etc.
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Embodiment 1
[0049] Add 1g 2-[benzyl (tert-butyl)amino]-1-[4-hydroxy-3-(hydroxymethyl)phenyl]ethanol, 15ml methanol, 0.9g potassium carbonate, 0.72g bromine to a 50ml three-necked flask Ethane, stirred and heated to 35°C~40°C, reacted for 24h. Filtrate while hot, concentrate the filtrate to dryness under reduced pressure at 40°C~50°C, add 5ml of ethanol to the concentrated residue, heat up to reflux, stir to dissolve, cool down to -5°C~0°C, stir and crystallize for 3h, filter, 50°C~60°C Dry in vacuo for 8 hours to obtain 0.65g salbutamol impurity with a purity of 99.377%. The spectrogram is shown in the attached Figure 4 , yield 59.9%.
[0050] Confirmation of chemical structure
[0051] Mass spectrometry was performed on an Agilent technologies 6120 mass spectrometer with methanol as solvent. Its spectrogram is attached figure 1 shown.
[0052] Molecular weight calculated: 357.0; MS m / e (M+H): 358.0.
[0053] H NMR spectrum ( 1 H-NMR) was performed on a Bruker AVANCE-400 nuclear m...
Embodiment 2
[0058] Add 10g 2-[benzyl (tert-butyl)amino]-1-[4-hydroxy-3-(hydroxymethyl)phenyl]ethanol, 200ml ethyl acetate, 6.0g potassium carbonate, 8.2 g iodoethane, stirred and heated to 35°C~40°C, and reacted for 24h. Cool down to 20°C~25°C, add 100ml of water to wash and separate the liquid, add 100ml of saturated sodium chloride to the organic phase to wash and separate the liquid, concentrate the organic phase at 40°C~50°C to dryness under reduced pressure, add 30ml of methanol to the concentrated residue and heat up to reflux, Stir to dissolve, lower the temperature to -5°C~0°C, stir and crystallize for 3h, filter, and vacuum dry at 50°C~60°C for 8h to obtain 5.8g of albuterol impurities with a purity of 99.121% and a yield of 53.5%.
Embodiment 3
[0060] Add 10g 2-[benzyl (tert-butyl)amino]-1-[4-hydroxy-3-(hydroxymethyl)phenyl]ethanol, 200ml dichloromethane, 6.3g pyridine, 7.0g Bromoethane, stirred and heated to reflux, reacted for 24h. Cool down to 20°C~25°C, add 100ml of water to wash and separate the liquid, add 100ml of saturated sodium chloride to the organic phase to wash and separate the liquid, concentrate the organic phase to dryness under reduced pressure at 35°C~40°C, add 30ml of methanol to the concentrated residue and heat up to reflux. Stir to dissolve, lower the temperature to -5°C~0°C, stir and crystallize for 3 hours, filter, and vacuum dry at 50°C~60°C for 8 hours to obtain 6.0 g of albuterol impurities with a purity of 99.340% and a yield of 55.3%.
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