Method for synthesizing On-DNA pyrimidine compound
A technology of compound and carbonyl compound, applied in the field of coding compound library, can solve the problem of no report
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Embodiment 1
[0059] The synthesis of embodiment 1, On-DNA pyrimidine compound
[0060] Step 1, On-DNA α, the synthesis of β-unsaturated carbonyl compound
[0061]
[0062] Dissolve the On-DNA arylethanone compound (1) in 250 mM, pH=9.4 boric acid buffer solution to prepare a 1 mM concentration solution (20 μL, 20 nmol), and add benzaldehyde (4000 nmol, 200 equivalents, 200mM DMSO), sodium hydroxide (10000nmol, 500 equivalents, 500mM double-distilled water), mix well, and react at 30°C for 2 hours.
[0063] After the reaction is completed, carry out ethanol precipitation: add a total volume of 10% 5M sodium chloride solution to the reacted solution, and then continue to add 3 times the total volume of absolute ethanol, shake evenly, and place the reaction in dry ice to freeze After 0.5 hours, centrifuge at 12000rpm for half an hour, pour off the supernatant, and dissolve the remaining precipitate with deionized water to obtain a solution of On-DNAα,β-unsaturated carbonyl compound (2). ...
Embodiment 2
[0067] The synthesis of embodiment 2, On-DNA pyrimidine compound
[0068] Step 1, On-DNA α, the synthesis of β-unsaturated carbonyl compound
[0069]
[0070] Dissolve the On-DNA arylethanone compound (1) in 250 mM, pH=9.4 boric acid buffer solution to prepare a 1 mM concentration solution (20 μL, 20 nmol), and add benzaldehyde (4000 nmol, 200 equivalents, 200mM DMSO), sodium hydroxide (10000nmol, 500 equivalents, 500mM double-distilled water), mix well, and react at 30°C for 2 hours.
[0071] After the reaction is completed, carry out ethanol precipitation: add a total volume of 10% 5M sodium chloride solution to the reacted solution, and then continue to add 3 times the total volume of absolute ethanol, shake evenly, and place the reaction in dry ice to freeze After 0.5 hours, centrifuge at 12000rpm for half an hour, pour off the supernatant, and dissolve the remaining precipitate with deionized water to obtain a solution of On-DNAα,β-unsaturated carbonyl compound (2). ...
Embodiment 3
[0075] The synthesis of embodiment 3, On-DNA pyrimidine compound
[0076]
[0077] 21 kinds of On-DNA α, β-unsaturated carbonyl compounds were dissolved in 250mM, pH = 9.4 boric acid buffer solution to prepare a 1mM concentration solution (20μL, 20nmol), and phenylamidine (8000nmol, 400 equivalents) was added to the solution , 400mM DMSO), NaOH (10000nmol, 500 equivalents, 1000mM double distilled water) were mixed uniformly, and after reacting at 80°C for 2 hours, simple iodine (2000nmol, 100 equivalents, 200mM THF) was added to the system and mixed uniformly, and reacted at 30°C for 1 hour.
[0078] After the reaction is completed, carry out ethanol precipitation: add a total volume of 10% 5M sodium chloride solution to the reacted solution, and then continue to add 3 times the total volume of absolute ethanol, shake evenly, and place the reaction in dry ice to freeze After 0.5 hours, centrifuge at 12,000rpm for half an hour, pour off the supernatant, and dissolve the rema...
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