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Method for breeding new rice variety with wild rice genetic background

A technology for wild rice and new varieties, applied in biochemical equipment and methods, botanical equipment and methods, microbial measurement/inspection, etc., can solve the problems of phenotype bias towards wild rice, distant parentage, long segregation generations, etc. , to achieve the effect of solving the serious problem of homogenization, overcoming technical difficulties and bottlenecks, and expanding the genetic background

Pending Publication Date: 2021-06-11
上海中科荃银分子育种技术有限公司
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, due to the distant genetic relationship between wild rice and cultivated rice, the genetic background is quite different, resulting in many defects in the offspring produced by cross breeding between the two, for example, there are huge differences in phenotypic segregation, phenotype bias towards wild rice, linkage burden, and segregation generations. Long and difficult to stabilize genetic traits, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The rice new variety breeding process of embodiment 1

[0057] Step 1): Using the cultivated rice variety ("WYG" with good comprehensive agronomic traits) as the female parent and the AA-type wild rice variety (AA-type common wild rice "374") as the male parent, perform conventional sexual crossing to obtain the hybrid F1 generation;

[0058] Step 2): Planting the hybrid F1 generation obtained in step 1); using the hybrid F1 generation as the female parent and the cultivated rice "WYG" as the male parent, perform the first backcross to obtain BC1F1 generation seeds;

[0059] Step 3): plant the BC1F1 generation seeds obtained in step 2), obtain the BC1F1 generation plant population, and take the leaves of the BC1F1 generation plant population at the seedling stage for low-coverage whole-genome sequencing to obtain the genotype of each BC1F1 generation plant Atlas;

[0060] The specific operation process for low-coverage whole-genome sequencing and obtaining genotype ma...

Embodiment 2

[0084] The rice new variety breeding process of embodiment 2

[0085] Step 1): Using the cultivated rice variety ("Wushan Simiao" with good comprehensive agronomic characteristics) as the female parent and the AA-type wild rice variety (AA-type common wild rice "W3105") as the male parent, carry out conventional sexual hybridization to obtain a hybrid F1 generation;

[0086] Step 2): planting the F1 generation of the hybrid obtained in step 1); using the F1 generation of the hybrid as the female parent and the cultivated rice "Wushan Si Miao" as the male parent, perform the first backcross to obtain BC1F1 generation seeds;

[0087] Step 3): plant the BC1F1 generation seeds obtained in step 2), obtain the BC1F1 generation plant population, and take the leaves of the BC1F1 generation plant population at the seedling stage for low-coverage whole-genome sequencing to obtain the genotype of each BC1F1 generation plant Atlas;

[0088] The specific operation process for low-coverag...

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Abstract

The invention relates to a method for breeding a new rice variety with a wild rice genetic background. The method utilizes a rice genomics technology to rapidly and accurately breed the new rice variety with the wild rice genetic background. More importantly, the inventor of the invention improves screening conditions in the breeding process, so that the technical difficulty and the bottleneck in the crossbreeding process of the wild rice and cultivated rice are overcome, the new rice variety with the wild rice genetic background can be bred, a cultivated rice genetic background is effectively expanded, the serious problem of existing variety homogenization is solved, novel germplasm resources can be created favorably, the yield of hybrid rice is increased, and breakthrough varieties are obtained.

Description

technical field [0001] The invention relates to a method for breeding a new rice variety with a genetic background of wild rice, in particular to a method for rapidly and accurately breeding a new rice variety with a genetic background of wild rice by using rice genomics technology, and belongs to the technical field of rice breeding. Background technique [0002] Rice is the main food crop in my country. Since the 21st century, the global natural environment has been deteriorating and extreme weather has occurred frequently, which puts forward new requirements for the selection and breeding of new rice varieties. [0003] Due to the serious homogeneity of rice varieties at present, extreme natural climate disasters will bring a devastating blow to the high and stable yield of rice. Therefore, expanding the genetic background of parents and creating germplasm resources with rich genetic background are the main methods for breeding new high-yield hybrid rice varieties. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/6895
CPCA01H1/02A01H1/04C12Q1/6895
Inventor 方玉李潜龙王阿红陈洪俭
Owner 上海中科荃银分子育种技术有限公司
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