Transparent hydrogel based on chitosan and preparation method thereof
A chitosan and hydrogel technology, applied in the field of chitosan-based transparent hydrogel and its preparation, can solve the problems of reducing the biocompatibility of the system, complicated synthesis process, etc., and achieve good mechanical properties and high transparency Effect
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[0036] The present invention also provides a kind of preparation method of above-mentioned transparent hydrogel based on chitosan, comprising: S) mixing chitosan and aldehyde small molecule cross-linking agent in water, heating reaction, obtains chitosan-based Transparent hydrogel.
[0037] Wherein, the present invention does not have special restriction to the source of all raw materials, just can be commercially available; The kind and the ratio of described chitosan and aldehyde small molecule cross-linking agent are all the same as above-mentioned, do not repeat them here; In the present invention, unless otherwise specified, the amount of chitosan is calculated according to its single chain link.
[0038] Chitosan and aldehyde small molecule cross-linking agent are mixed in water; in the present invention, chitosan is preferably dispersed in water first, and then the aqueous solution of aldehyde small molecule cross-linking agent is added under heating conditions, and sti...
Embodiment 1
[0045] (1) Take 0.12 g of glyoxylic acid monohydrate and dissolve it in 3 mL of deionized water at room temperature to obtain solution A.
[0046] (2) Disperse 0.2 g of high-viscosity chitosan (>400 mPa s) in 3 mL of deionized water, add the entire volume of solution A under heating at 50 ° C and magnetic stirring, and continue heating and stirring for 20 min to obtain a clear yellowish viscous Solution B.
[0047] (3) Take solution B and sonicate it for one minute to remove small air bubbles, draw it into a 1mL syringe, cover the syringe cap, and place it in an oven at 70°C.
[0048] (4) After 4 hours, the syringe was taken out, the gel was pushed out, soaked in excess deionized water, and a chitosan-based transparent hydrogel (CSG hydrogel) was obtained after swelling.
[0049] Wherein, in this reaction system, the mass concentration of chitosan is 3.16%, the mass concentration of glyoxylic acid is 1.90%, and the mass ratio of chitosan and glyoxylic acid is 1:1.05 (chitosan...
Embodiment 2
[0051] (1) Take 0.09 g of glyoxylic acid monohydrate and dissolve it in 3 mL of deionized water at room temperature to obtain solution A.
[0052] (2) Disperse 0.2 g of high-viscosity chitosan (>400 mPa s) in 3 mL of deionized water, add the entire volume of solution A under heating at 50 ° C and magnetic stirring, and continue heating and stirring for 20 min to obtain a clear yellowish viscous Solution B.
[0053] (3) Take solution B and sonicate it for one minute to remove small air bubbles, draw it into a 1mL syringe, cover the syringe cap, and place it in an oven at 70°C.
[0054] (4) After 4 hours, take out the syringe, push out the gel, soak in excess deionized water, and obtain CSG hydrogel after swelling.
[0055] Wherein, in this reaction system, the feeding mass concentration of chitosan is 3.17%, the feeding mass concentration of glyoxylic acid is 1.43%, and the amount ratio of the feeding material of chitosan and glyoxylic acid is 1:0.78 (chitosan Sugar is calcul...
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