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Entomopathogenic fungus as well as screening method and application thereof

A technology of pathogenic fungi and screening methods, which is applied in the field of entomopathogenic fungi and their screening, can solve problems such as the impact of control effects and the reduction of spore production, and achieve the effects of good pest control, large spore production, and avoidance of adverse effects

Active Publication Date: 2021-06-11
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are two defects in utilizing the existing Metarhizium anisopliae as biological pesticides to prevent and control pests: one, the Metarhizium anisopliae that are more used in the biological control of pests are Metarhizium anisopliae (Metarhizium anisopliae), Metarhizium anisopliae (Metarhizium anisopliae) robertsii) and other Metarhizium anisopliae that produce green spores, but it is difficult to distinguish different Metarhizium anisopliae strains on the market, and sometimes there are shoddy phenomena; secondly, for agricultural pests, temperature is the main factor affecting whether it breaks out Factors, the spore production of Metarhizium anisopliae, which is widely used at present, will decrease at high temperature, and its control effect will be affected

Method used

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  • Entomopathogenic fungus as well as screening method and application thereof
  • Entomopathogenic fungus as well as screening method and application thereof
  • Entomopathogenic fungus as well as screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Isolation and identification of entomopathogenic fungi

[0031] (1) Isolation of strain SGSF125

[0032] The strains were isolated from the under-forest litter of Changbai Mountain. First, the collected air-dried litter samples were pulverized to a granular shape, and filtered with a filter screen to obtain particles with a particle size of 100-200 μm in the litter samples. Then use the plate dilution method to mix the granular sample with sterile water in a mass ratio of 1:100 to prepare a granular suspension. Then use a pipette gun to draw 50 μL, 100 μL, 150 μL, 200 μL and 250 μL respectively and spread them on the PDA medium plate, spread them with a coating rod until the particles are evenly dispersed, and place them at room temperature. out, the colonies were picked to PDA plates for purification. After purification and culture, a fungus was obtained, which was numbered SGSF125.

[0033] (2) Isolation of strain SGSF355

[0034] The strains were isol...

Embodiment 2

[0045] Embodiment 2, the biological characteristic of novel metarhizium anisopliae

[0046] 1. Test method

[0047] (1) Determination of strain growth rate and spore production at different temperatures

[0048] Punch holes in the colonies of strain SGSF125 and strain SGSF355 with a hole puncher, then transfer them to PDA medium, and place them in incubators at 20°C, 25°C, and 30°C, respectively. Each treatment was repeated three times, and every day The colony growth diameter was measured regularly for a total of 14 days. At the end of the measurement experiment, 5 mL of sterilized 1% Tween 80 aqueous solution was added to the plate, the surface spores were scraped off, and after the excess mycelium and agar were filtered out with sterile cotton, the amount of spore production was determined using a hemocytometer.

[0049] (2) Determination of strain growth rate and spore production under different pH

[0050] Holes were punched on the colonies of strain SGSF125 and strain...

Embodiment 3

[0068] Embodiment 3, Metarhizium anisopliae insect-resistant activity assay

[0069] (1) Test method

[0070] First prepare the spore suspension, inoculate the strain on the PDA medium and cultivate for 14 days, add 5 mL of sterilized 0.05% Tween 80 aqueous solution on the plate, and scrape the surface spores. After filtering out excess mycelium and agar with sterilized cotton, use a hemocytometer to count and adjust the spore suspension to a final concentration of 1×10 8 spores / mL.

[0071] Soak the two-spotted firefly beetle (adult) in 1×10 8 spores / mL concentration of spore suspension for 5s, and then transferred to a Erlenmeyer flask filled with corn kernels for observation, and each treatment was inoculated with 35 adults. Soak mung bean weevil (adult) in 1×10 8 spores / mL concentration of spore suspension for 5 s, and then transferred to a Erlenmeyer flask containing mung beans with a brush for observation, and 20 adults were inoculated for each treatment. Use a brus...

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Abstract

The invention belongs to the field of biological control, and provides an entomopathogenic fungus as well as a screening method and the application thereof. In order to solve the problems of pesticide residue, poor selectivity, short lasting period and the like existing in pest control through chemical pesticides, two fungi are obtained through separation, recognition proves that the two fungi are both new species of entomopathogenic fungi metarhizium anisopliae, the biological characteristics and insect-resistant activity of the two fungi are respectively determined, and experiments prove that the two fungi have a good application prospect. The entomopathogenic fungus disclosed by the invention has good insect-resistant activity and wide infection range, belongs to non-host specialized metarhizium anisopliae, and can be used as a biocontrol strain. The invention not only provides alternative strains for biological control of pests, but also solves the problem that the control effect is influenced due to the fact that the sporulation quantity of the existing entomopathogenic fungus metarhizium anisopliae is greatly influenced by temperature rise.

Description

technical field [0001] The invention belongs to the field of biological control, in particular to an entomopathogenic fungus and a screening method and application thereof. Background technique [0002] At present, chemical control methods are mainly used for the control of field pests. Chemical pesticides will generate a large amount of waste during the production process, which may endanger the environment and human health. In use, although contact-killing chemical pesticides have obvious efficacy, they need to be used in large quantities to ensure that they are in direct contact with pests, which is likely to cause waste or pesticide residues and other related problems. In addition, some chemical pesticides also have problems such as poor selectivity, short duration of effect, and easy generation of drug resistance. The application of biological pesticides can partially alleviate these problems. [0003] The use of entomopathogenic bacteria (such as Beauveria bassiana a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCC12N1/14A01N63/30
Inventor 徐利剑高思禹张李香孟威王庆贵
Owner HEILONGJIANG UNIV
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