Novel lactic acid bacteria and its use for treating or/and preventing hyperuricemia and alcoholic liver injury
A technology of alcoholic liver injury and lactic acid bacteria, applied in the field of novel lactic acid bacteria, treatment or/or prevention of hyperuricemia and alcoholic liver injury, can solve problems such as side effects, allergic syndrome, patient death, etc., achieve good tolerance, Effect of treatment or/and hyperuricemia and alcoholic liver injury, prevention of hyperuricemia and alcoholic liver injury
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Embodiment 1
[0045] Example 1: Cell Culture
[0046] The human hepatoma cell line HepG2 cell line (deposit number: BCRC 60177, hereinafter referred to as HepG2 cells) was purchased from the Biological Resource Conservation and Research Center, Hsinchu Food Industry Research Institute, Taiwan, China, and the medium used was MEM medium supplemented with 10% fetal bovine serum, 1% optional amino acid and 1.5g sodium bicarbonate.
[0047] Mouse macrophage RAW264.7 (deposit number: BCRC 60001, hereinafter referred to as RAW264.7 cells) was purchased from the Biological Resource Conservation and Research Center, Hsinchu Food Industry Research Institute, Taiwan, China, and the medium used was DMEM medium supplemented with 10% fetal Bovine serum, 1% non-essential amino acids and 1.85g sodium bicarbonate.
Embodiment 2
[0048] Example 2: Anti-inflammatory test
[0049] HepG2 cells (2 x 10 5 cell / well) and RAW264.7 cells (1 X 105 cell / well) were inoculated in a 24-well dish. When the cells grew to 80%, they were washed with phosphate buffered saline, and 800 μl of new cell culture medium was added. Add 100 μl of endotoxin lipopolysaccharide (100 ng / ml) and 100 μl of lactic acid bacteria solution (10 9 CFU / ml), wherein, the lactic acid bacteria solution was first washed with phosphate buffer, then the supernatant was removed, and then reconstituted with cell culture medium, the well plate was placed in a 5% carbon dioxide environment for 24 hours, and the cell supernatant was collected. ELISA kit (purchased from BioLegend) was used to analyze the content of TNF-α and IL-6 in cell supernatant, and ELISA kit (BD OptEIA Set Human IL-8, purchased from BD Biosciences) was used to analyze cell supernatant The content of IL-8 in the liquid, the results are shown in Table 1 below.
[0050] Table 1...
Embodiment 3
[0053] Example 3: Alcohol tolerance test
[0054] The TTL 8-16 strains and TTL 6-10 strains disclosed in the present invention were activated once, the culture medium was lactic acid bacteria MRS medium containing 0.05% L-cysteine, the culture condition was 37°C, and the culture time was 16 hours. Take 100 μl of the cultured bacterial liquid and inoculate it into 4 ml MRS medium without alcohol, and 4 ml MRS medium containing final alcohol concentration of 1%, 5%, 10%, 15%, the culture temperature is 37 ℃, and the culture time is 16 Hour. The bacterial liquid after the cultivation was serially diluted, and the bacterial volume was calculated by plate counting. The culture medium was lactic acid bacteria MRS agar medium containing 0.05% L-cysteine, the culture temperature was 37°C, and the culture time was 48 hours. The results are as follows: shown in Table 2.
[0055] Table 2: Results of alcohol tolerance test
[0056]
[0057] It can be seen from the results in Table 2...
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