Method for constructing HPLC (High Performance Liquid Chromatography) characteristic chromatogram of heart-nourishing hypnotic capsule

A technology of characteristic map and construction method, which is applied in the field of HPLC characteristic map construction of Yangxin Anmian Capsules, can solve the problems of cumbersome operation, low sensitivity of detection equipment, specificity and poor reproducibility of content determination method, and achieve good precision and method stable effect

Active Publication Date: 2021-06-11
吉林修正药业新药开发有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, the qualitative research on schisandra, jujube kernel, and licorice has poor specificity and reproducibility of the content determination method, and the sensitivity of the detection equipment is low, and the operation is cumbersome

Method used

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  • Method for constructing HPLC (High Performance Liquid Chromatography) characteristic chromatogram of heart-nourishing hypnotic capsule
  • Method for constructing HPLC (High Performance Liquid Chromatography) characteristic chromatogram of heart-nourishing hypnotic capsule
  • Method for constructing HPLC (High Performance Liquid Chromatography) characteristic chromatogram of heart-nourishing hypnotic capsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: A method for constructing the HPlC characteristic map of Yangxin Anmian Capsules

[0069] Instruments: UltiMate 3000 HPLC, UV DAD detector; Mettler AE240 1 / 100,000 analytical balance (Shanghai Mettler-Toledo Instrument Co., Ltd.); AB204-E electronic balance (Shanghai Mettler-Toledo Instrument Co., Ltd.), etc.

[0070] Reagents: methanol, etc. are analytically pure, phosphoric acid and acetonitrile are chromatographically pure, and water is ultrapure water.

[0071] Reagents: mangiferin, liquiritin, schisandrin A, schisandrin A, schisandrin B (all from China National Institute for Food and Drug Identification).

[0072] Method and Results

[0073] (1) Take about 1g of the contents of Yangxin Anmian Capsules, accurately weighed, accurately add 25ml of 70% ethanol, weighed, ultrasonically treat for 30 minutes, let cool, make up the lost weight with methanol, filter, and take the filtrate , that is;

[0074] (2) Preparation of reference solution: take an appr...

Embodiment 2

[0079] Example 2: A construction method of HPlC characteristic map of Yangxin Anmian Capsules

[0080] Instruments: UltiMate 3000 HPLC, UV DAD detector; Mettler AE240 1 / 100,000 analytical balance (Shanghai Mettler-Toledo Instrument Co., Ltd.); AB204-E electronic balance (Shanghai Mettler-Toledo Instrument Co., Ltd.), etc.

[0081] Reagents: methanol, etc. are analytically pure, phosphoric acid and acetonitrile are chromatographically pure, and water is ultrapure water.

[0082] Reagents: mangiferin, liquiritin, schisandrin A, schisandrin A, schisandrin B (all from China National Institute for Food and Drug Identification).

[0083] Method and Results

[0084] (1) Preparation of the test solution: take about 2 g of the content of Yangxin Anmian Capsules, accurately weighed, accurately add 25 ml of methanol, weigh, ultrasonically treat for 30 minutes, let cool, make up the lost weight with methanol, filter After that, take the continued filtrate to obtain;

[0085] (2) Prepa...

Embodiment 3

[0090] Example 3: A method for constructing the HPlC characteristic map of Yangxin Anmian Capsules

[0091] Instruments: UltiMate 3000 HPLC, UV DAD detector; Mettler AE240 1 / 100,000 analytical balance (Shanghai Mettler-Toledo Instrument Co., Ltd.); AB204-E electronic balance (Shanghai Mettler-Toledo Instrument Co., Ltd.), etc.

[0092] Reagents: methanol, etc. are analytically pure, phosphoric acid and acetonitrile are chromatographically pure, and water is ultrapure water.

[0093] Reagents: mangiferin, liquiritin, schisandrin A, schisandrin A, schisandrin B (all from China National Institute for Food and Drug Identification).

[0094] Method and Results

[0095] (1) Preparation of the test solution: take about 3 g of the content of Yangxin Anmian Capsules, accurately weighed, accurately add 50 ml of methanol, weigh, ultrasonically treat for 40 minutes, let cool, make up the lost weight with methanol, filter After that, take the continued filtrate to obtain;

[0096] (2) ...

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Abstract

The invention provides a method for constructing HPLC characteristic chromatogram of heart-nourishing hypnotic capsule, which comprises: A) taking a heart-nourishing hypnotic capsule content, and dissolving the heart-nourishing hypnotic capsule content with a solvent to obtain a liquid to be detected, wherein the solvent is methanol or 50-70% ethanol; b) measuring the liquid to be measured by adopting a high performance liquid chromatography to obtain the HPLC characteristic chromatogram of the heart-nourishing hypnotic capsule, wherein chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 column, a mobile phase A is acetonitrile, a mobile phase B is 0.05% phosphoric acid solution, and gradient elution is carried out. According to the method, a high performance liquid chromatography method is adopted, a 0.05% phosphoric acid solution of acetonitrile is selected as a mobile phase for gradient elution, mangiferin, liquiritin, schizandrin, schizandrin A and schisandrin B are taken as reference substances, the HPLC characteristic chromatogram of the heart-nourishing hypnotic capsule content is established, the repeatability and precision are good, the method is stable and reliable, and the quality of the heart-nourishing hypnotic capsule content can be controlled.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a method for constructing an HPLC characteristic map of Yangxin Anmian Capsules. Background technique [0002] The composition of Chinese patent medicine Yangxin Anmian Capsules consists of deer heart (fresh), deer blood (fresh), Poria cocos, jujube seed, longan meat, schisandra, etc., and its quality standards are recorded in WS-5740(B-0740)-2014Z Among them, it includes TLC identification of Schisandra chinensis, TLC identification of Suanzaoren, TLC identification of licorice, TLC identification of buffalo horn concentrated powder; content determination of Schisandrin B by dual-wavelength scanning. [0003] Traditional Chinese medicine compound is the main form of TCM clinical medication, and its quality is the key to clinical efficacy. How to establish a scientific and reasonable quality control method that reflects the characteristics of traditional Chinese m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N30/8686Y02A50/30
Inventor 徐云徐建展月李朋莲张新君郑广晶崔宪利王卉玲王昭兵
Owner 吉林修正药业新药开发有限公司
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