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Microbial composition and application thereof in promoting germination and growth of highland barley seeds

A technology of microbial composition and microbial strains is applied in the field of agricultural microorganisms, which can solve the problems of declining quality of agricultural products, rare research, damage to the ecological environment, etc., and achieves the effects of strong acid resistance, excellent IAA production ability, and excellent phosphorus dissolving ability.

Active Publication Date: 2021-06-15
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Excessive application of chemical fertilizers will not only effectively increase the yield of highland barley, but will also lead to an imbalance in the proportion of soil nutrients, a decline in the quality of agricultural products, and damage the local ecological environment.
[0003] Biological bacterial fertilizer is a new type of environment-friendly fertilizer, mainly composed of plant growth promoting rhizobacteria (PGPR) that promotes plant growth. At present, the application of PGPR bacterial fertilizer is mainly concentrated in fruits and vegetables. On the other hand, research on barley is relatively rare

Method used

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  • Microbial composition and application thereof in promoting germination and growth of highland barley seeds
  • Microbial composition and application thereof in promoting germination and growth of highland barley seeds
  • Microbial composition and application thereof in promoting germination and growth of highland barley seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Screening and identification of microorganisms

[0047] The media and reagents involved in the full text are as follows:

[0048] (1) LB medium (1 L): 5 g of yeast powder, 10 g of peptone, 10 g of NaCl, pH=7.0.

[0049] (2) PKO solid medium (1L): glucose 10g, (NH 4 ) 2 SO4 0.5g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O 0.003g, MnSO 4 4H 2 O 0.003g, MgSO 4 ·7H 2 O 0.3g, Ca(PO 4 ) 2 5g, 15g agar, pH7.0~7.2. PKO liquid medium does not contain agar, and the other components are the same.

[0050] (3) Salkowski reagent: 0.5M FeCl 3 5g, concentrated H 2 SO 4 150mL, dilute to 250mL.

[0051] (4) Molybdenum antimony anti-mixing color developer: Mix A solution and B solution according to the volume ratio of 1:2 for 150mL, and dilute to 250mL.

[0052] Solution A: molybdate solution: slowly add ① to 300mL of ③, add ② and mix well.

[0053] ①: Dissolve 13g ammonium molybdate (NH 4 ) 6 Mo 7 o 24 4H 2 O in 100mL water;

[0054] ②: Dissolve 0.35g pota...

Embodiment 2

[0089] Embodiment 2: The influence of microbial combination mode on the germination of highland barley seeds

[0090] 1. Inoculate PD4, PC2 and PB7 obtained through screening into 50mL LB liquid medium respectively, and cultivate them in a constant temperature shaking incubator at 30°C and 180r / min for 48 hours, and then dilute the cultured bacteria liquids to a concentration of 10 6 、10 7 、10 8 、10 9 CFU / mL, combined according to the combination in Table 6. Combination mode and volume ratio refer to: mix the seed liquids of PD4, PC2 and PB7 with the same concentration of viable bacteria according to the corresponding volume ratio to prepare the microbial composition. The unit of viable bacteria concentration is CFU / mL. It should be noted that the inventor has done a lot of combination experiments, and Table 6 only shows a representative part of them.

[0091] The combination of microorganisms in each group in Table 6

[0092]

[0093]

[0094] 2. Select dry highl...

Embodiment 3

[0099] Embodiment three: the influence of different components of microbial composition on the germination of highland barley seeds

[0100] 1. Prepare the microbial composition suspension in the manner of Example two groups 33 (the ratio of the three is 1:1:1, and the concentration of viable bacteria is 10 8 CFU / mL). After centrifugation, the centrifuged supernatant, cell pellet, and cell disrupted liquid were obtained. At the same time, clean water was used as the blank control group, and the freshly prepared, sterilized LB liquid medium not inoculated with microorganisms was used as the positive control group.

[0101] There were 30 seeds in each group, and the highland barley seeds were completely immersed in the above-mentioned groups for 2 hours, then the seeds were taken out, placed on moistened sterilized filter paper, and cultivated in an incubator with a humidity of 90% at 25°C for 7 days. After the cultivation, the seed germination rate, seedling stem length, root...

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Abstract

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a microbial composition and application thereof in promoting germination and growth of highland barley seeds. According to the specific technical scheme, the microbial composition at least comprises at least two of Rahnella aquatilis, Enterobacter ludwigii and Enterobacter cloacae. Three new microbes are screened out, the acid resistance is high, IAA can be produced under the condition that no precursor substance exists, and when the precursor substance is provided, the IAA production capacity is very excellent; and the phosphate solubilizing capability is excellent, and certain siderophore producing capability is also realized. The microbial preparation is prepared from three microbes, is used for germination, rooting promotion and seedling growth of seeds, especially highland barley seeds, and has remarkable effects.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, in particular to a microbial composition and its application in promoting the germination and growth of highland barley seeds. Background technique [0002] Highland barley is a cereal crop of the genus Barley in the Poaceae family. It is mainly distributed in China's Tibet, Qinghai, Sichuan, Yunnan and other places. It is one of the main food sources for local residents. Existing highland barley planting method is relatively simple, and systematic planting is weaker, causes the output of highland barley to be lower. In recent years, in order to pursue high yields of highland barley, agricultural production in these areas generally uses excessive amounts of chemical fertilizers. Excessive application of chemical fertilizers will not effectively increase the yield of highland barley, but will also lead to an imbalance in the proportion of soil nutrients, a decline in the quali...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P21/00A01P1/00A01P3/00C12R1/01
CPCC12N1/20A01N63/20
Inventor 李东艾爽陈意超周攀
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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