Preparation and application of Chinese cobra bite pig model

A technology of Chinese cobra and cobra, which is applied in the field of medicine, can solve the problems of large animal models of Chinese cobra bites, and achieve the effects of large application value, strong repeatability, and short construction period

Inactive Publication Date: 2021-06-25
何冬凌 +6
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To solve the problem that there is no large animal model of Chinese cobra bites in the prior art

Method used

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  • Preparation and application of Chinese cobra bite pig model
  • Preparation and application of Chinese cobra bite pig model
  • Preparation and application of Chinese cobra bite pig model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Preparation of pig model bitten by Chinese cobra.

[0018] 1. Materials and reagents: 31 healthy mini-pigs, male or female, weighing about 15kg each, sourced from Guangxi Miniature Pig Breeding Center of Guangxi University, experimental animals approved by the Animal Ethics Committee of the school, experimental animal production license: SCXK Gui 2018-0003. They were raised in the Experimental Animal Center of Guangxi Medical University, license number SYXK Gui 2014-0003, temperature 20°C-25°C, relative humidity 50%-65%. The freeze-dried powder of Chinese cobra venom comes from the Snake Venom Research Institute of Guangxi Medical University.

[0019] 2. Method

[0020] 2.1 Explore the median lethal dose of intramuscular injection of cobra snake venom in pigs: select 25 pigs of uniform size, male or female, and divide them into 5 groups with different concentrations of cobra venom. The cobra venom concentration gradient, from high to low, was 8.64, 7.2, 6,...

Embodiment 2

[0037] Embodiment 2: the application of cobra bite pig model

[0038] The cobra bitten pig model established by the invention is used to solve the problem that there is no cobra bitten animal model in the prior art. This model has the advantages of high success rate, strong reproducibility, and stable pathological changes. Moreover, the condition of cobra bites is relatively long, and the animal model obtained by this production method is helpful for dynamic analysis of the development process of cobra bites, which is closer to the physiological and pathological dynamic process of human beings bitten by cobras, and can be well in line with clinical practice. Provide a basis for diagnosis, provide a good animal model for the study of cobra venom metabolism changes in the human body and the mechanism of muscle necrosis caused by snake venom, and have great application value in the identification of snakebite types, clinical manifestations after bites, and precise treatment. And...

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Abstract

The invention discloses a manufacturing method of a Chinese cobra bite pig model and half lethal dose (LD50) of cobra venom to pigs and application of the cobra venom to an animal model. Cobra venom freeze-dried powder is dissolved and then injected into pig leg muscles according to 2mg/kg, and a target animal model can be obtained after 6 hours, so that the problem that no Chinese cobra bite model of large animals in the prior art is solved. The model has the advantages of high success rate, strong repeatability, stable pathological change and the like. The cobra bite disease condition is long, the model simulates human being bite by the cobra, the physiological and pathological dynamic process of the human being bite by the cobra can be well close to, and dynamic analysis of the disease clinical manifestation and mechanism of the cobra bite is facilitated. The model can provide a good animal model for researching metabolic change of Chinese cobra venom in a human body and a muscle necrosis mechanism, and provides an innovative technical method support for researching clinical characterization of cobra bite, pathophysiological mechanism, precise treatment, screening of anti-venomous snake bite medicines, evaluation of curative effect of anti-venom serum and the like.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a production method and application of a pig model bitten by Chinese cobras. Background technique [0002] Venomous snake bites (snakebites) are listed by the World Health Organization as one of the important tropical / subtropical diseases that are easily overlooked. There are about 5 million snakebites in the world every year, resulting in about 25,000-125,000 deaths. According to the epidemiological survey, the incidence rate (16.68%) of Chinese cobra bites ranks second in snakebite, and cobra is one of the most widely distributed venomous snake species in my country. Cobra venom is a mixed poison, including neurotoxins and blood circulation toxins. Neurotoxins manifest as dyspnea, respiratory paralysis, and heart failure; blood circulation poisons manifest as heart damage, showing toxic cardiomyopathy. speed, shock until death. The clinical diagnosis and treatment of cobra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/027A61K49/0008A01K2207/20A01K2227/108A01K2267/03
Inventor 何冬凌廖明黄智莫财锋宋鹏书程晓阳李亚兰
Owner 何冬凌
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