Extraction method of ganoderma lucidum spore powder polysaccharide

A technology of Ganoderma lucidum spore powder and extraction method, which is applied in the field of deep processing of natural products, can solve the problems of low polysaccharide extraction rate of broken wall Ganoderma lucidum spore powder, cumbersome steps of broken wall Ganoderma lucidum spore powder, low acquisition rate of broken wall Ganoderma lucidum spore powder, etc. Achieve the effect of retaining biological activity, low cost and easy operation

Inactive Publication Date: 2021-06-25
贵州高山生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the steps for obtaining the broken ganoderma lucidum spore powder are cumbersome, and the acquisition rate of the broken gan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for extracting ganoderma lucidum spore powder polysaccharide, comprising the following steps:

[0030] (1) Pre-treatment: select fresh Ganoderma lucidum spore wet thallus without insects and no rot, remove impurities, wash and send it to the dryer for drying; the drying is to dry the Ganoderma lucidum spores until the water content is 35% ;

[0031] (2) Wall-breaking treatment: put the Ganoderma lucidum spores pre-treated in step (1) into the compound enzyme solution for enzymolysis and breaking the wall, and then carry out microwave wall-breaking treatment after stirring evenly to obtain the broken Ganoderma lucidum spores; the compound enzyme It is two or more of cellulase, chitinase and glucosidase; the temperature of the enzymolysis breaking the wall is 37°C, and the time is 120min; the power of the microwave breaking the wall is 600W, and the temperature is 85℃, the time is 155s;

[0032] (3) Ultrasonic extraction: add pure water to the broken ganoderma ...

Embodiment 2

[0036] A method for extracting ganoderma lucidum spore powder polysaccharide, comprising the following steps:

[0037] (1) Pre-treatment: select fresh Ganoderma lucidum spore wet thallus without insects and rot, remove impurities, wash and send it to the dryer for drying; the drying is to dry the Ganoderma lucidum spores until the water content is 45% ;

[0038] (2) Wall-breaking treatment: put the Ganoderma lucidum spores pre-treated in step (1) into the compound enzyme solution for enzymolysis and breaking the wall, and then carry out microwave wall-breaking treatment after stirring evenly to obtain the broken Ganoderma lucidum spores; the compound enzyme It is two or more of cellulase, chitinase and glucosidase; the temperature of the enzymolysis breaking the wall is 38.5°C, and the time is 130min; the power of the microwave breaking the wall is 700W, and the temperature is 95℃, the time is 175s;

[0039] (3) Ultrasonic extraction: add pure water to the broken ganoderma l...

Embodiment 3

[0043] A method for extracting ganoderma lucidum spore powder polysaccharide, comprising the following steps:

[0044] (1) Pre-treatment: select fresh Ganoderma lucidum spore wet thallus without insects and no rot, remove impurities, wash and send it to the dryer for drying; the drying is to dry the Ganoderma lucidum spores until the water content is 37% ;

[0045] (2) Wall-breaking treatment: put the Ganoderma lucidum spores pre-treated in step (1) into the compound enzyme solution for enzymolysis and breaking the wall, and then carry out microwave wall-breaking treatment after stirring evenly to obtain the broken Ganoderma lucidum spores; the compound enzyme It is two or more of cellulase, chitinase and glucosidase; the temperature of the enzymolysis breaking the wall is 37.5°C, and the time is 122min; the power of the microwave breaking the wall is 620W, and the temperature is 87℃, the time is 160s;

[0046] (3) Ultrasonic extraction: add pure water to the broken ganoderm...

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PUM

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Abstract

The invention discloses an extraction method of ganoderma lucidum spore powder polysaccharide. The method comprises the following steps: pretreatment; wall breaking treatment; ultrasonic extraction; concentration; and freeze-drying and the like. According to the ganoderma spore wall breaking method, enzymolysis and microwave wall breaking are adopted, the wall breaking rate of ganoderma spores is effectively increased and reaches up to 99%, and effective components in the raw materials can be effectively reserved; and through ultrasonic extraction and vacuum freeze drying, the biological activity of the ganoderma lucidum spore powder polysaccharide is effectively reserved, the influence of physical and chemical factors such as solvents, temperature and pressure is avoided, the ganoderma lucidum spore powder polysaccharide which is fragrant and sweet in cane sugar, high in purity and free of organic solvent residues is finally obtained, and the extraction rate of the ganoderma lucidum spore powder polysaccharide is high and reaches up to 99%.

Description

technical field [0001] The invention belongs to the technical field of deep processing of natural products, and in particular relates to a method for extracting polysaccharides from ganoderma lucidum spore powder. Background technique [0002] Ganoderma lucidum is a large medicinal fungus. Sweet in nature and flat in taste, Guixin, Lung, Liver, Kidney channels. It is the dry fruiting body of Ganoderma lucidum (Leyss.exFr.) Karst. or Ganodermasinense Zhao, XuetZhang, which belongs to the basidiomycete class Polyporaceae fungus. The extremely fine particles ejected from the cap of Ganoderma lucidum in the mature stage of growth are Ganoderma lucidum spore powder, which is called basidiospore in biology. It is the germ cell of Ganoderma lucidum and has all the genetically active substances of Ganoderma lucidum, and its main active ingredients are high. Ganoderma lucidum is produced 75 times, and its main components are polysaccharides, triterpenes, proteins, amino acids, nucl...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 李鹏张林陈荔李敏王森柳学方华刚邓桂秀胡登峰张超然罗倩杨玲洪江向准程志飞罗丹
Owner 贵州高山生物科技有限公司
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