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Construction method and application of mycoplasma bovis mouse mastitis model

A technology of Mycoplasma bovis and a construction method, which can be applied to preparations for in vivo tests, medical preparations containing active ingredients, sexual diseases, etc., can solve the obstacles to the research and development of antibiotic substitutes for the anti-inflammatory treatment plan of dairy cow mastitis, and the lack of pathological damage. Evaluate models and other issues to achieve good practical application value, good repeatability and convenience, and the effect of promoting development

Active Publication Date: 2021-06-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for cow mastitis caused by Mycoplasma bovis, there is still a lack of an effective pathological damage evaluation model
The structural specificity of the blood-milk barrier makes the treatment of mycoplasma mastitis different from the model of bovine mycoplasma pneumonia, which brings obstacles to the anti-inflammatory treatment of dairy cow mastitis and the development of antibiotic alternatives

Method used

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  • Construction method and application of mycoplasma bovis mouse mastitis model
  • Construction method and application of mycoplasma bovis mouse mastitis model
  • Construction method and application of mycoplasma bovis mouse mastitis model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1, be used for the strain selection of infection

[0047] The Mycoplasma bovis NMH7 strain is an isolate of Mycoplasma bovis isolated from a milk sample from a dairy farm in Inner Mongolia in 2018 by the inventor's research group. After epidemiological and pathogenicity analysis, a large amount of background information has been obtained on the genotype characteristics and pathogenicity of Mycoplasma bovis NMH7 strain.

[0048] The results of multi-gene locus sequence analysis show that the Mycoplasma bovis NMH7 strain belongs to a newly discovered genotype in my country, ST173, and its background information is included in the MLST database (https: / / pubmlst.org / bigsdb?db=pubmlst_mbovis_isolates&page=query) (detailed See doi: 10.1016 / j.prevetmed.2020.105106). Mycoplasma bovis NMH7 strain is documented at: https: / / pubmlst.org / bigsdb? db=pubmlst_mbovis_isolates&page=query, ID number is 1015.

[0049]Furthermore, the inventor tested milk samples from dairy far...

Embodiment 2

[0052] Embodiment 2, mycoplasma bovis infection causes mouse mastitis

[0053] 1. Construction of mastitis model mice

[0054] The infection solution was inoculated into lactating CD-1 mice 5 days after delivery. The way of inoculation is through the milk duct of mice. The inoculation site is the fourth pair of mammary glands of mice. The infection solution was obtained by suspending Mycoplasma bovis NMH7 strain in sterile phosphate buffer solution, and the concentration of Mycoplasma bovis was 1×10 6 CFU / ml. The inoculum volume was 100 μl of infection solution per mammary gland.

[0055] 2. Control mice

[0056] Sterile phosphate buffered saline was inoculated into lactating CD-1 mice 5 days after delivery. The way of inoculation is through the milk duct of mice. The inoculation site is the fourth pair of mammary glands of mice. The inoculation volume was 100 μl of sterile phosphate buffered saline per mammary gland.

[0057] 3. Detection of relevant indicators

[0...

Embodiment 3

[0072] Example 3, the protective effect experiment of active oxygen scavenger on mouse mastitis caused by Mycoplasma bovis infection

[0073] NAC (N-acetyl-L-cysteine): an active oxygen scavenger, Sigma Company, USA, product number A7250.

[0074] NAC was diluted with sterile saline to obtain a NAC solution with a concentration of 10 mM.

[0075] 1. The treatment method of M.bovis group + NAC group

[0076] 1. Take lactating CD-1 mice 5 days after delivery, and inject NAC solution (150 mg NAC / kg body weight) intraperitoneally.

[0077] 2. Inoculate the infection solution 12 hours after the intraperitoneal injection. The way of inoculation is through the milk duct of mice. The inoculation site is the fourth pair of mammary glands of mice. The infection solution was obtained by suspending Mycoplasma bovis NMH7 strain in sterile phosphate buffer solution, and the concentration of Mycoplasma bovis was 1×10 6 CFU / ml. The inoculum volume was 100 μl of infection solution per ma...

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Abstract

The invention discloses a construction method and application of a mycoplasma bovis mouse mastitis model. The construction method of the mouse mastitis model comprises the following step of inoculating an infection solution to mammary glands of a mouse in a lactation period, wherein the infection solution is obtained by suspending mycoplasma bovis NMH7 strains by using a sterile buffer solution, specifically, the inoculation manner can be inoculation through mouse milk ducts, and specifically, the mammary glands of the mouse are the fourth pair of mammary glands of the mouse. The invention also relates to the application of N-acetyl-L-cysteine in preparation of medicines for treating mycoplasma bovis cow mastitis. The model constructed by the method can simulate pathological changes of cow mammary glands when the mycoplasma bovis cow mastitis occurs clinically, good repeatability and convenience are achieved, and good practical application value is achieved.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and in particular relates to a construction method and application of a mycoplasma bovis mouse mastitis model. Background technique [0002] Mycoplasma bovis (M.bovis) can cause various diseases such as mastitis, pneumonia, arthritis and otitis media in cattle. Dairy cow mastitis is caused by pathogenic microorganism infection or other physical and chemical stimuli, causing redness, swelling, heat, pain and other inflammatory reactions in the mammary glands of dairy cows. It is a disease that causes the most serious economic losses in the dairy industry worldwide. Mycoplasma bovis mastitis in dairy cows can lead to systemic symptoms such as fever in dairy cows, and inflammatory reactions such as redness, swelling, heat and pain in the udder area. Mycoplasma bovis mastitis in dairy cows can lead to a sharp drop in milk production or cessation of milk production, a decrease in fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61K49/00A61K31/198A61P15/14A61P31/04
CPCA01K67/027A61K49/0008A61K31/198A61P15/14A61P31/04A01K2207/20A01K2227/105A01K2267/03
Inventor 韩博刘洋高健王登峰石玉祥
Owner CHINA AGRI UNIV
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