A kind of preparation method of wild pineapple polysaccharide

A technology of wild pineapple and polysaccharide, applied in the field of medicine, can solve problems such as low polysaccharide content, and achieve the effect of improving biological activity, improving purity, and enhancing blood sugar reduction.

Active Publication Date: 2022-01-21
HAINAN YUNHAO BIOLOGICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the polysaccharide content that above-mentioned preparation method obtains is all lower

Method used

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  • A kind of preparation method of wild pineapple polysaccharide
  • A kind of preparation method of wild pineapple polysaccharide
  • A kind of preparation method of wild pineapple polysaccharide

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[0031] The invention discloses a preparation method of wild pineapple polysaccharide, and those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.

[0032] The raw materials, reagents or instruments used in the preparation method of the wild pineapple polysaccharide provided by the invention can be purchased from the market.

Embodiment 1

[0035] Take 80kg of wild pineapple fruit, wash it, steam for 30 minutes, take it out, dry it at 60°C for 30 minutes, steam it for the second time for 30 minutes, take it out, dry it at 60°C for 30 minutes, take it out, get 13.5kg of wild pineapple dried fruit, crush it, and get wild pineapple dry powder 13.2 kg, add 10 times of water, heat to 90°C, extract for 60 minutes, filter to obtain the filtrate ①; add 8 times of water to the filter residue, heat to above 90°C, extract for 30 minutes, filter to obtain the filtrate ②, combine the filtrates ① and ②, and concentrate to The solid content is 40%, vacuum-dried at 50° C., pulverized, and passed through a 40-mesh sieve to obtain 3.23 kg of wild pineapple extract.

[0036] Add 5 times of water to the wild spinach extract, stir to dissolve, centrifuge at 8000 rpm for 6 minutes, take the clear liquid, pass the solution through a 0.22nm membrane, add 95% ethanol until the alcohol concentration of the solution reaches 70%, centrifuge ...

Embodiment 2

[0039]Take 80kg of wild pineapple fruit, wash it, steam it for 105 minutes, take it out, dry it at 80°C for 75 minutes, steam it for the second time for 75 minutes, take it out, dry it at 80°C for 75 minutes, take it out, and get 13.8kg of dried wild pineapple fruit, crush it, add 14 times of water, Heat to 95°C, extract for 90 minutes, filter to obtain the filtrate ①; add 11.5 times of water to the filter residue, heat to above 95°C, extract for 60 minutes, filter to obtain the filtrate ②, combine the filtrates ① and ②, concentrate until the solid content is 50%, 65 ℃ vacuum drying, crushing, and passing through a 80-mesh sieve to obtain 3.45kg of wild pineapple extract.

[0040] Add 10 times water to the wild spinach extract, stir to dissolve, centrifuge at 8000 rpm for 8 minutes, take the clear liquid, pass the solution through a 0.22nm membrane, add 95% ethanol until the alcohol concentration of the solution reaches 70%, centrifuge at 8000 rpm for 8 minutes, The precipitat...

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Abstract

The invention relates to the technical field of medicine, in particular to a preparation method of wild pineapple polysaccharide. The preparation method comprises the following steps: steaming and drying the wild pineapple fruit to obtain dried wild pineapple fruit; pulverizing the dried wild pineapple fruit, and extracting the dried fruit powder with water, concentrating, drying and pulverizing to obtain the wild pineapple water extract; The pineapple water extract is dissolved in water, centrifuged, and the supernatant is filtered to obtain a clear liquid; the clear liquid is subjected to alcohol precipitation, the precipitate is dried, crushed and sieved to obtain the primary product; the primary product is dissolved in water, and after filtration Use neutral protease to carry out enzymatic hydrolysis, inactivate the enzyme, and obtain the retentate after ultrafiltration; concentrate the retentate, carry out alcohol precipitation again, dry and pulverize. The purity of the wild pineapple polysaccharide extracted by the invention reaches more than 90 percent, the biological activity of the wild pineapple polysaccharide is improved, and the effect of lowering blood sugar and blood fat of the wild pineapple polysaccharide is enhanced.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a preparation method of wild pineapple polysaccharide. Background technique [0002] Wild pineapple is the fruit of the pandanus plant. Because the shape is similar to pineapple, Lingnan folks call it wild pineapple. Wild pineapples are widely distributed in Hainan and are rich in resources. They can be eaten as wild fruits among the people. The pulp is sweet, but the pulp is rich in vascular tissue and has a poor taste. Therefore, it is not very popular. It is used for the treatment of diseases and coughs in Hainan . The roots, leaves and other parts of Pandanus can be used for medicinal purposes. It has the functions of clearing away heat and dampness, diuresis, relieving the exterior, promoting qi and calming the liver. Modern pharmacological studies have shown that the active ingredients of wild pineapple can regulate lipid metabolism in diabetic mice, reduce fat accumula...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P3/10A61P3/06
CPCC08B37/0003A61P3/10A61P3/06
Inventor 伍曾利
Owner HAINAN YUNHAO BIOLOGICAL TECH CO LTD
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