Application of WRKY55 transcription factor in plant salt resistance

A stress-resistant, sequence-listed technology, applied in the biological field, can solve the problems of little research on WRKY transcription factors

Pending Publication Date: 2021-07-13
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors found that at present, research is mainly on a small number of model organisms such as Arabidopsis, and there is little research on WRKY transcription factors in other plants

Method used

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  • Application of WRKY55 transcription factor in plant salt resistance
  • Application of WRKY55 transcription factor in plant salt resistance
  • Application of WRKY55 transcription factor in plant salt resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Bioinformatics analysis of sweet sorghum WRKY55 gene

[0061] 1 test material

[0062] Sweet sorghum (Sb02g011050), and wild rice with short anthers (XM_015839135.1), Brachypodium distachyda (XM_014901792.1), zucchini (XM_023659186.1), corn (XM_020540231.2), cassava ( WRKY protein sequences of XM_021761582.1), Anthurium (KY597634.1), Vitis vinifera (KY411919.1), Arabidopsis (AT2G40740).

[0063] 2 test method

[0064] Firstly, the primary structure and physicochemical properties of SbWRKY55 protein were analyzed by software ExPASy. The detection of the signal peptide and the prediction of the transmembrane region use the software Signal4.1 and TMHMM. SMART online software can predict the structure and functional domains of SbWRKY55. The homologous sequences of SbWRKY55 were obtained from NCBI BLASTp on the Internet, and then the homologous sequences were compared in terms of proteins with software such as MegAlign and a phylogenetic tree reflecting the ph...

Embodiment 2

[0078] Example 2 Cloning, Vector Construction and Subcellular Localization of Sweet Sorghum WRKY55 Gene

[0079] 1 Test materials and material handling

[0080] Seeds of sweet sorghum salt-tolerant and high-sugar inbred line M-81E (gifted by Shandong Academy of Agricultural Sciences), tobacco (Nicotiana benthamiana) seeds, Escherichia coli strain (DH5α), Agrobacterium strain (GV3101), cloning vector pEASY-Blunt3 simple, pROKII-GFP expression vector driven by CaMV35S promoter, etc.

[0081]Select sweet sorghum seeds with similar sizes and full grains, and wash them in a mesh bag with running water for 12 hours. The sand was washed with tap water and divided into pots. Then plant the seeds evenly in the sand, 8 plants per pot, with a depth of about 2cm, not too shallow, to prevent them from being washed out when watering. Water tap water once a day until the hole at the bottom of the flower pot can flow out, which is counted as thoroughly watered. After emergence, pour 1 / 2 H...

Embodiment 3

[0114] Example 3: Overexpression of Arabidopsis SbWRKY55 and the acquisition of AtWRKY55 mutant lines

[0115] 1 test material

[0116] Columbia ecotype (Col-0) Arabidopsis plants were purchased from Tair website. Arabidopsis WRKY55 (AT2G40740) mutants were wrky55-1 (SALK_084192), wrky55-2 (SALK_021677), wrky55-3 (SALK_121437), wrky55 -4 (SALK_084288), wrky55-5 (SALK_082916), wrky55-6 (CS838667).

[0117] 2 test method

[0118] 2.1 Obtaining Arabidopsis SbWRKY55 overexpression lines

[0119] After the inflorescence of Arabidopsis thaliana was infiltrated with Agrobacterium pROKⅡ-GFP-SbWRKY55, it was covered with a black plastic film and cultured in the dark for about 24 hours, and then the black plastic wrap was removed for normal culture. After about 7 days, the second infection is carried out, and the culture is continued in an artificial climate incubator (22°C-16h light / 18°C-8h dark). After about 24 hours, the black plastic film was removed, and the cultivation was con...

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Abstract

The invention provides an application of a WRKY55 transcription factor in plant salt resistance, and belongs to the technical field of biology. The invention reports the salt resistance negative regulation related protein WRKY55 and the coding gene thereof for the first time. The research finds that the expression quantity of the WRKY55 gene in sweet sorghum is remarkably reduced under the salt stress condition, the WRKY55 gene is introduced into arabidopsis thaliana, functional identification is performed on the WRKY55 gene, it is indicated that overexpression of the WRKY55 enables the salt resistance of plants to be reduced, and therefore it is indicated that the WRKY55 gene plays a negative regulation role in the salt resistance of the plants, and a foundation is provided for cultivation of resistant plants.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of WRKY55 transcription factor in plant salt resistance. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Soil salinization is a worldwide problem. At present, the saline-alkali land area in the world is vast, which is 9.5xl0 8 hm 2 , and there is an increasing trend. Improper irrigation measures in agricultural activities have exacerbated the generation of salinized soil, and the area has continued to expand. A salinized environment is harmful to most crops. Therefore, comprehensive treatment of saline soil to increase the area of ​​cultivated land...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/84C12N15/11A01H5/00A01H6/46A01H6/20A01H6/60A01H6/38A01H6/54A01H6/34A01H6/00
CPCC07K14/415C12N15/8273
Inventor 隋娜郑洪祥李思敏
Owner SHANDONG NORMAL UNIV
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